Imbewu yeTyalo(Enkulu) I-PCR Kit II-UNG ethe ngqo (ngaphandle kweZixhobo zokuSampula)
Inkcazelo yeKit:
Ukusetyenziswa ngokuthe ngqo kwembewu ye-lysate njengethempleyithi yokukhulisa i-PCR, akukho kukhutshwa kwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
Iinkcazelo
Le mveliso isebenzisa inkqubo yokusabela ye-lysis ekhethekileyo, enokuthi ikhulule ngokukhawuleza i-DNA ye-genomic kwiisampuli zembewu yezityalo kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol (ezifana ne: irayisi, ingqolowa, icuba, umbona, iimbotyi zesoya, njl.Ukuze kuhlangatyezwane neemfuno zeemvavanyo ezahlukeneyo zokubona, le khithi inokusebenzisa iindidi ezininzi zeesampulu (ezifana nembewu epheleleyo, ukusikwa kwezicubu ezincinci, okanye iisampuli zomhlaba zembewu ezininzi) kwiimvavanyo.Phakathi kwazo, kwiisampulu zembewu ezisafuna ukuntshuliswa, unokukhetha ukusika ukusikwa kwezicubu ezincinci (1-5mg) ngaphandle kwe-embryo yembewu yovavanyo;kwimifuniselo ekufuneka isampuliwe kwinani elikhulu leisampulu, ungakhetha ukuxuba iisampulu ezininzi kwaye uzidibanise.Emva kokugaya kwiinqununu ezinobubanzi obumalunga ne-0.5mm, uvavanyo lunokwenziwa (ubuncinci i-0.1% yesampuli ekujoliswe kuyo inokufunyanwa).
Iinkcukacha
50T,200T,500T,2000T
Amacandelo ekhithi
| Tyala iMbewu eNgqo PCR Kit II-UNG Imbewu yokutyala (Enkulu) I-PCR Kit-UNG ethe ngqo | |||||
| Ukuqulunqwa kweKit | TP-0314T | TP-03141 | TP-03142 | TP-03143 | |
| 50 T | 200T | 500T | 2000T | ||
| Icandelo I | Isithinteli SP1 | 30ml | 120ml | 100ml×3 | 400ml×3 |
| Isithinteli SP2 | 3ml | 10ml | 25ml | 100ml | |
| I-6 × i-DNA yokulayisha i-Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
| Icandelo II | 2 × Imbewu yePCR KululaTMUmxube(UNG) | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| Imanyuwali | 1 | 1 | 1 | 1 | |
Iimpawu kunye neenzuzo
- Akukho mfuneko yokuchitha ixesha kunye nokucoca i-DNA ebiza kakhulu iyafuneka.
- Imfuno yesampulu incinci, thatha nje imbewu enye.
- Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
-Isistim ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezela okunamandla kwi-PCR reaction inhibitors.
- Inkqubo yePCR yokuchasana nongcoliseko 2× Imbewu PCR EasyTMUkuxuba (UNG) kunokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.
Ukuhamba komsebenzi
Iimeko zokugcina
1.Ezothutho
Yonke inkqubo yokuthutha i-ice box yobushushu obuphantsi, ukuqinisekisa ukuba i-kit Part II ikwimo ye-<4 ℃.
2.Ugcino
Inxalenye I yale khithi kufuneka igcinwe kwindawo yokushisa okanye i-2-8 ° C.
Icandelo II kufuneka ligcinwe kwi -20°C.
