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I-SARS-CoV-2 Nucleic Acid yeKit (iMultiplex PCR Fluorescent Probe Method)

Inkcazelo yeKit:

 

◮ I-PCR ethe ngqo:Akukho mfuneko yekhithi yokutsalwa kwe-nucleic acid kunye neNkqubo, igqityiwe i-96 isampuli kwiyure eyi-1 okanye njalo.

 

◮ Imfuneko yesixhobo esiphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu:Kufuneka kuphela inkqubo yePCR yexesha lokwenyani .

 

◮ I-LoD kunye novakalelo oluphezulu:Ukubona ubuntununtunu kunokufikelela kwelona nqanaba lisezantsi njengeekopi ezingama-500/ml.

amandla angaphambili


Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa okucetywayo

Le khithi isebenzisa itekhnoloji ye-RT PCR yexesha lokwenyani (rRT-PCR) ukufunyaniswa okusemgangathweni kwe-SARS-CoV-2 nucleic acids kwiisampulu ze-nasopharyngeal okanye ze-oropharyngeal swab.

Umgaqo wovavanyo

Le primer kunye neeseti zophando ziyilelwe ukuchongwa kolandelelwano olugciniweyo lwe-SARS-CoV-2 (ORF1ab gene kunye ne-N gene).Ikhithi iqulethe uLawulo lwangaphakathi olusetyenziselwa ukuhlola umgangatho womzekelo.
Itekhnoloji ye-PCR yexesha lokwenyani isebenzisa i-polymerase chain reaction (PCR) ukukhulisa ithagethi ethile.
ulandelelwano kunye nokujolisa kwi-probes ezithile zokubona i-RNA eyandisiweyo.Iiprobe zibhalwe nge
intatheli ye-fluorescent kunye nedayi yokucima.

Iinkcukacha eziKhawulezayo

Iinkcukacha:48 Rxns/kit, 96 Rxns/kit

◮ Ukucaciswa:96.72%

Uvakalelo:97.92%

◮ Iintlobo zesampulu ezisebenzayo
I-Nasopharyngeal Swab okanye i-Oropharyngeal Swab
Isixhobo esisebenzayo
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

 

Amacandelo

Hayi.

Icandelo

Isixa

Amacandelo aphambili

48Rxns

96 Rxns

1 I-agent yokukhululwa kwe-Nucleic acid 1.4mL / ityhubhu 2 iityhubhu 5.3 mL/ibhotile 1 ibhotile I-surfactant
2 Umkhuseli we-RNA 27 μL/ityhubhu ityhubhu e-1 53 μL/ityhubhu ityhubhu e-1 I-RNase inhibitor
3 Isisombululo sokusabela kwe-SARS-CoV-2 800 μL/ityhubhu ityhubhu e-1 1600 μL/ityhubhu ityhubhu e-1 Iprimer, probe, reaction buffer, dNTP
4 Umxube we-enzyme ye-SARS-CoV-2 80 μL/ityhubhu ityhubhu e-1 160 μL/ityhubhu ityhubhu e-1 Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV
5 Ulawulo olulungileyo lwe-SARS-CoV-2 100 μL/ityhubhu ityhubhu e-1 100 μL/ityhubhu ityhubhu e-1 I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA
6 Ulawulo olubi lwe-SARS-CoV-2 1200 μL/ityhubhu ityhubhu e-1 1200 μL/ityhubhu ityhubhu e-1 TE buffer

Iimpawu&izinto eziluncedo

I-PCR ethe ngqo
Akukho mfuneko yekhithi yokutsalwa kwe-nucleic acid kunye neNkqubo, igqityiwe i-96 isampuli kwiyure eyi-1 okanye njalo.
Imfuno yezixhobo eziphantsi kunye nokusetyenziswa okuguquguqukayo kakhulu
Kufuneka kuphela inkqubo yePCR yexesha lokwenyani.
I-LoD kunye novakalelo oluphezulu
Ukubona ubuntununtunu kunokufikelela kwelona nqanaba lisezantsi njengeekopi ezingama-500/ml.
igophe lokukhulisa-RT-qPCR

Kusetyenziswa kwanjani

Indlela yoku-1: Indlela ye-PCR ethe ngqo

Indlela yesi-2: I-Viral RNA Isolation + PCR

Indlela yoku-1: Indlela ye-PCR ethe ngqo usebenzisaIarhente yokukhupha iSampuli  

 

Inkqubo yokukhangela

Iinkqubo zokuSebenza

Iinkqubo

Cold Chain Transport and Storage

Itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;

yokugcina
ugcino2

Beka ubomi kwishelufa

1 unyaka

Ukuodola Ulwazi

Ukuodola ulwazi

Ividiyo yokuSebenza ngokukhawuleza


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