I-China SARS-CoV-2 Ikhithi yokuFumana i-Nucleic Acid (iNdlela yokuHlola i-Multiplex PCR yeFluorescent) uMenzi kunye noMboneleli |I-Foregene

I-SARS-CoV-2 Nucleic Acid yeKit (iMultiplex PCR Fluorescent Probe Method)

I-SARS-CoV-2 Nucleic Acid Detection Kit (iMultiplex PCR Fluorescent Probe Method) Umfanekiso okhoyo

Inkcazelo yeKit:

◮ I-PCR ethe ngqo:Akukho mfuneko yekhithi yokutsalwa kwe-nucleic acid kunye neNkqubo, igqityiwe i-96 isampuli kwiyure eyi-1 okanye njalo.

◮ Imfuneko yesixhobo esiphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu:Kufuneka kuphela inkqubo yePCR yexesha lokwenyani .

◮ I-LoD kunye novakalelo oluphezulu:Ukubona ubuntununtunu kunokufikelela kwelona nqanaba lisezantsi njengeekopi ezingama-500/ml.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa okucetywayo

Le khithi isebenzisa itekhnoloji ye-RT PCR yexesha lokwenyani (rRT-PCR) ukufunyaniswa okusemgangathweni kwe-SARS-CoV-2 nucleic acids kwiisampulu ze-nasopharyngeal okanye ze-oropharyngeal swab.

Umgaqo wovavanyo

Le primer kunye neeseti zophando ziyilelwe ukuchongwa kolandelelwano olugciniweyo lwe-SARS-CoV-2 (ORF1ab gene kunye ne-N gene).Ikhithi iqulethe uLawulo lwangaphakathi olusetyenziselwa ukuhlola umgangatho womzekelo.

Itekhnoloji ye-PCR yexesha lokwenyani isebenzisa i-polymerase chain reaction (PCR) ukukhulisa ithagethi ethile.

ulandelelwano kunye nokujolisa kwi-probes ezithile zokubona i-RNA eyandisiweyo.Iiprobe zibhalwe nge

intatheli ye-fluorescent kunye ne-quencher dyes.

Iinkcukacha eziKhawulezayo

◮Iinkcukacha:48 Rxns/kit, 96 Rxns/kit

◮ Ukucaciswa:96.72%

Uvakalelo:97.92%

◮ Iintlobo zesampulu ezisebenzayo

I-Nasopharyngeal Swab okanye i-Oropharyngeal Swab

◮Isixhobo esisebenzayo

ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

Amacandelo

Hayi.	Icandelo	Isixa				Amacandelo aphambili
Hayi.	Icandelo	48Rxns		96 Rxns		Amacandelo aphambili
1	I-agent yokukhululwa kwe-Nucleic acid	1.4mL / ityhubhu	2 iityhubhu	5.3 mL/ibhotile	1 ibhotile	I-surfactant
2	Umkhuseli we-RNA	27 μL/ityhubhu	ityhubhu e-1	53 μL/ityhubhu	ityhubhu e-1	I-RNase inhibitor
3	Isisombululo sokusabela kwe-SARS-CoV-2	800 μL/ityhubhu	ityhubhu e-1	1600 μL/ityhubhu	ityhubhu e-1	Iprimer, probe, reaction buffer, dNTP
4	Umxube we-enzyme ye-SARS-CoV-2	80 μL/ityhubhu	ityhubhu e-1	160 μL/ityhubhu	ityhubhu e-1	Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV
5	Ulawulo olulungileyo lwe-SARS-CoV-2	100 μL/ityhubhu	ityhubhu e-1	100 μL/ityhubhu	ityhubhu e-1	I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA
6	Ulawulo olubi lwe-SARS-CoV-2	1200 μL/ityhubhu	ityhubhu e-1	1200 μL/ityhubhu	ityhubhu e-1	TE buffer