I-SARS-CoV-2 Nucleic Detection Kit (i-Multiplex PCR yeFluorescent Probe Method)

I-SARS-CoV-2 Nucleic Detection Kit (i-Multiplex PCR yeFluorescent Probe Method)

Inkcazo yeKiti:

Le khithi isebenzisa ubuchwepheshe be-Real PC ye-RTR (rRT-PCR) ekufumaneni umgangatho we-SARS-CoV-2 (i-ORF1ab yemfuza kunye nohlobo lwe-N) yeecicic acid kwi-nasopharyngeal okanye kwi-oropharyngeal swab sampuli, ngendlela ye-multiplex PCR fluorescent probe. ulawulo lwangaphakathi lokuvavanya umgangatho wesampulu, ke ngoko akukho mfuneko yokuhlanjululwa kwe-acid ye-acid, unokugqibezela uvavanyo kwiyure e-1, ngakumbi ifanelekile ukubonwa ngokukhawuleza okukhulu.


Iinkcukacha Product

Iimpawu zemveliso

Ukucaciswa

IiRxns ezingama-48, iiRxns ezingama-96

Hayi.

Icandelo

Isixa

Amacandelo aphambili

IiRxns ezingama-48

IiRxns ezingama-96

1 Iarhente yokukhupha iasidi yeNucleic 1.4mL / ityhubhu Iityhubhu ezi-2 5.3 ml / ibhotile Ibhotile e-1 Ukudibana
2 Ukukhusela i-RNA 27 μL / ityhubhu 1 ityhubhu 53 μL / ityhubhu 1 ityhubhu RNase inhibitor
3 Isisombululo se-SARS-CoV-2 800 μL / ityhubhu 1 ityhubhu 1600 μL / ityhubhu 1 ityhubhu I-primer, probe, buffer yokuphendula, dNTP
4 Umxube we-enzyme ye-SARS-CoV-2 80 μL / ityhubhu 1 ityhubhu 160 μL / ityhubhu 1 ityhubhu Isiqalo esishushu seTaq enzyme, iM-MLV enzyme
5 Ulawulo oluqinisekileyo lwe-SARS-CoV-2 100 μL / ityhubhu 1 ityhubhu 100 μL / ityhubhu 1 ityhubhu Iplasmid ephindayo eneqhekeza ekujoliswe kulo, iRNA
6 Ulawulo olubi lwe-SARS-CoV-2 1200 μL / ityhubhu 1 ityhubhu 1200 μL / ityhubhu 1 ityhubhu Isikhuseli se-TE

Iimpawu kunye nezibonelelo

Ngokukhawuleza kwaye kulula.
Gwema ukulahleka kwe-acid ye-nucleic.
Umda wokubhaqa osezantsi kunye novakalelo oluphezulu.
Imfuno yezixhobo ezisezantsi kunye nokusetyenziswa okuguqukayo kakhulu.

Ukufumanisa ubuntununtunu kunokufikelela kwelona zinga liphantsi njengeekopi ezingama-500 / mL).

I-GCE / mL Ixabiso leCt
Uhlobo lweN Uhlobo lwe-ORF1ab
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21.86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 N / A N / A
2.00E-01 N / A N / A
I-NC N / A N / A

Umgama wokukhulisa ijika

amplification curve-RT-qPCR

Kusetyenziswa kwanjani

1. Isixhobo esisebenzayo

covid-19 nucleic acid detection kit1

2. Inkqubo yokufumanisa

covid-19 nucleic acid detection kit2

Ukulungiswa kwesampula: I-Naopharyngeal okanye i-oropharyngeal swab isampulu (kuphela inyathelo elinye lokusebenza) (Lindela imizuzu eyi-10)

I-PCR amplificaton (<= 55min)

Ukuhamba komsebenzi:

covid-19 nucleic acid detection kit4

Ukugcina

itywiniwe ekukhanyeni kwaye igcinwe kwi -20 ± 5 ℃;

storage
storage2

Beka ubomi kwishelufa: 1 unyaka


  • Egqithileyo
  • Okulandelayo:

  • Bhala umyalezo wakho apha uze uyithumele kuthi