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I-Animal miRNA Isolation Kit Ukutsalwa kweMicroRNA kunye neeKits zokuCoca kwizilwanyana
Ngokukhawuleza nangokufanelekileyo ukukhupha amaqhekeza amancinci e-RNA ye-20-200nt miRNA, i-siRNA, i-snRNA kwizicubu ezahlukeneyo zezilwanyana kunye neeseli.
◆Yonke le nkqubo iqhutywa kubushushu begumbi (15-25°C), ngaphandle kokuhlamba umkhenkce kunye ne-centrifugation yobushushu obuphantsi.
◆Isethi epheleleyo ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
◆Ikholomu yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukwenzela ukuba ikiti inokususa ukungcola kwe-DNA ye-genomic ngaphandle kokongeza i-DNase eyongezelelweyo.
◆Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
◆Isantya esikhawulezayo: Umsebenzi ulula kwaye unokugqitywa kwimizuzu engama-30.
◆Ukhuseleko: akukho kukhutshwa kwe-organic reagent efunekayo.
◆Umgangatho ophezulu: Iziqwenga ze-RNA ezikhutshiweyo zicocekile kakhulu, azinaprotheyini kunye nolunye ukungcola, kwaye zinokuhlangabezana neemfuno zomlambo osezantsi.
◆izicelo ezahlukeneyo zovavanyo
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