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Izicubu zezilwanyana I-PCR kit-UNG
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwiisampulu zezicubu zezilwanyana zokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo olukhulu lwemfuza.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65°C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo weDNA okhutshiweyo unokusetyenziswa njengetemplate yokuphendula kwePCR.
2×PCR KululaTM Umxube (i-UNG) usebenzisa i-DUTP endaweni ye-dTTP kwisiseko se-2×PCR KululaTM Hlanganisa, kwaye wongeza i-UNG enzyme (Uracil-N-glycosylase) enokuthotywa itemplate ene-DUTP ngaxeshanye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthoba imveliso yePCR equlathe uracil. I-enzyme ye-UNG ayizukuchaphazeleka kwitemplate engenayo i-uracil, ngaloo ndlela iqinisekisa ubungqangi kunye nokuchaneka kokukhulisa kunye nokuthintela ukubakho kweemveliso ze-PCR zongcoliseko ngexesha lovavanyo olukhulu lofuzo.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
