Izilwanyana zezilwanyana ngokuthe ngqo kwi-PCR Kit

  • Animal Tissue Direct PCR kit

    Izicubu zezilwanyana ezihambelana ngqo nekhithi yePCR

    Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwiisampulu zezicubu zezilwanyana zokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo olukhulu lwemfuza.

    Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65°C. Azikho ezinye iinkqubo ezinje ngeprotein nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwe-PCR.

    2× PCR KululaTM Umxube unonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye unokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokukuko nangokufanelekileyo. Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, i-reaction buffer, i-PCR optimizer kunye nokuzinzisa.

    I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.