Ngokuthe ngqo sebenzisa igazi elipheleleyo le-EDTA le-anticoagulated njengethempleyithi yokukhulisa i-PCR, enovakalelo oluphezulu kunye nokusebenza kakuhle kokukhulisa.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
◮Akukho mfuneko yokuhlanjululwa kwe-DNA ixesha elide kunye nexabiso eliphakamileyo, akukho nyango lwangaphambili, kunye nokusetyenziswa ngokuthe ngqo kwegazi elipheleleyo njenge-template ye-PCR.
◮Inkqubo inamandla okukhulisa amandla kwaye inokubona ngovakalelo i-genome kunye namaqhekeza e-DNA ekujoliswe kuwo angaphandle egazini.
◮2 × SuperEasyTMI-Mix (UNG) -EDTA inokunyamezela kwegazi: ukuya kwi-45% kwigazi lomntu kunye ne-20% kwigazi le-mouse.
◮Isampulu ivalwe ngokupheleleyo, ngoko ke akukho mfuneko yakuba nexhala malunga nokungcoliseka kwesampulu kunye neziphumo zobuxoki zePCR.Inkqubo yePCR echasene nongcoliseko
◮2 × SuperEasyTMI-Mix (UNG) -EDTA, iphelisa ngokufanelekileyo ukungcola okubangelwa iimveliso ze-PCR, kwaye iqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.