Iseli iyonke RNA yokuzahlula isixhobo

Iseli iyonke RNA yokuzahlula isixhobo

Inkcazo yeKiti:

Iyonke i-RNA inokucocwa kwimizuzu eli-11.

Ukucaciswa:  50 Preps, 200 Preps

Iimbonakalo:

-Inkqubo yonke iqhutywa kubushushu begumbi (15-25 ℃), ngaphandle kwebhafu yomkhenkce kunye neqondo lobushushu eliphantsi.

Ikhithi yonke i-RNase-Simahla, akukho sidingo sokukhathazeka malunga nokuthotywa kweRNA.

-I-DNA-Ukucoca ikholamu ibopha ngokukodwa iDNA, ukuze ikiti isuse ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.

 

 

 

 


  • :
  • Iinkcukacha Product

    Iimpawu zemveliso

    Inkcazo

    Le khithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu we-RNA iyonke kwiiseli ezityalwe kwi-96, 24, 12, kunye neepleyiti ezingama-6.

    Ikhithi ibonelela ngekholamu efanelekileyo yokuCoca i-DNA, enokuhlula ngokulula i-supernatant kunye ne-lysate yeseli, ukubopha nokususa i-genomic DNA. Umsebenzi ulula kwaye ugcina ixesha.

    TIkholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA ngefomula eyahlukileyo. Inani elikhulu leesampulu zinokuqwalaselwa ngaxeshanye.

    Izixhobo zekiti

    Ukwakhiwa kweKiti UMSEBENZI-03111 IMPENDULO-03114
    I-50 T 200 T
    I-Buffer cRL1 * I-25 ml 100 ml
    IBuffer cRL2 I-15 ml 60 ml
    Kwinqanaba RW1 * I-25 ml 100 ml
    IBuffer RW2 I-24 mL I-96 mL
    RNase-Simahla ddH2O I-10 ml I-40 mL
    Ikholamu ye-RNA-kuphela 50 200
    Ikholamu yokucoca i-DNA 50 200
    Umyalelo 1 1

    * Nceda unxibe iiglavu kwaye uthathe amanyathelo okhuselo ngexesha lokusebenza njengoko iBuffer cRL1 kunye neBuffer RW1 ziqulathe iityuwa ezikhathazayo ze-chaotropic.

    Iimpawu kunye nezibonelelo

    The Yonke le nkqubo iqhutywa kubushushu begumbi (15-25 ℃), ngaphandle kwebhafu yomkhenkce kunye neqondo lobushushu eliphantsi.
    ■ Yonke ikiti i-RNase-Simahla, akukho sidingo sokukhathazeka malunga nokuthotywa kweRNA.
    ■ Ikholamu yokucoca i-DNA ibopha ngokukodwa iDNA, ukuze ikiti isuse ungcoliseko lwe-genomic ye-DNA ngaphandle kokongeza i-DNase.
    ■ Ukuveliswa okuphezulu kweRNA: Ikholamu yeRNA kuphela kunye nefomula eyahlukileyo inokuyicoca ngokufanelekileyo iRNA.
    ■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eli-11.
    ■ Ukhuseleko: Akukho reagent yendalo ifunekayo.
    ■ Umgangatho ophezulu: I-RNA ehlanjululweyo isulungeke kakhulu, ayinazo iiprotheyini nezinye izinto ezingcolileyo, kwaye iyakwazi ukudibana novavanyo olulandelayo olulandelayo.

    123

    Isicelo seKiti

    Kukulungele ukukhutshwa kunye nokucocwa kwe-RNA iyonke kwiiseli ezinamasiko angama-96, 24, 12, kunye neepleyiti ezi-6.

    Ukuhamba komsebenzi

    cell total RNA

    Umzobo

    Cell Total RNA Isolation Kit Work Flow1

    Umzobo webhetri yebhetri ye-agarose yeSeli Iyonke i-RNA Isolate Kit inyange amanani alapha ngasentla eeseli, i-20μl yokunyusa ivolumu, thatha i-2μl ecociweyo ye-RNA 1%.

    Ukugcinwa kunye neshelf life

    Ikhithi ingagcinwa kangangeenyanga ezili-12 kubushushu begumbi (15-25 ℃) okanye 2-8 ℃ ixesha elide (iinyanga ezingama-24).

    I-Buffer cRL1 inokugcinwa kwi-4 ℃ kangangenyanga enye emva kokudibanisa i-2-hydroxy-1-ethanethiol (ngokuzithandela).


  • Egqithileyo
  • Okulandelayo:

  • Bhala umyalezo wakho apha uze uyithumele kuthi

    Imveliso iindidi