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Izicubu zezilwanyana ezihambelana ngqo nekhithi yePCR
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwiisampulu zezicubu zezilwanyana zokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo olukhulu lwemfuza.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65°C. Azikho ezinye iinkqubo ezinje ngeprotein nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwe-PCR.
2× PCR KululaTM Umxube unonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye unokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokukuko nangokufanelekileyo. Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, i-reaction buffer, i-PCR optimizer kunye nokuzinzisa.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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Imouse Tail SuperDirect PCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwimisila yemouse kunye neesampulu zeendlebe zeempuku zokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo olukhulu lwemfuza.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe ngaphakathi kwemizuzu eyi-10 ukuya kwi-30 kubushushu begumbi (20-25 ° C). Akukho shushu sifunekayo, kwaye ezinye iinkqubo ezinje ngeprotein kunye nokususwa kweRNA azifuneki. Ixabiso elikhutshiweyo le-DNA linokusetyenziswa njengeTemplate yokuphendula kwePCR.
I-2 × M-PCR Easy TM Mix inonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye inokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokufanelekileyo nangokukhulisa okuthile. Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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Izicubu zezilwanyana I-PCR kit-UNG
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwiisampulu zezicubu zezilwanyana zokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo olukhulu lwemfuza.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65°C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo weDNA okhutshiweyo unokusetyenziswa njengetemplate yokuphendula kwePCR.
2×PCR KululaTM Umxube (i-UNG) usebenzisa i-DUTP endaweni ye-dTTP kwisiseko se-2×PCR KululaTM Hlanganisa, kwaye wongeza i-UNG enzyme (Uracil-N-glycosylase) enokuthotywa itemplate ene-DUTP ngaxeshanye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthoba imveliso yePCR equlathe uracil. I-enzyme ye-UNG ayizukuchaphazeleka kwitemplate engenayo i-uracil, ngaloo ndlela iqinisekisa ubungqangi kunye nokuchaneka kokukhulisa kunye nokuthintela ukubakho kweemveliso ze-PCR zongcoliseko ngexesha lovavanyo olukhulu lofuzo.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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Iseli ngqo ye-RT qPCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer enokuthi ikhuphe i-RNA ngokukhawuleza kwiisampulu zeseli ezenzelwe i-RT-qPCR reaction, ngaloo ndlela ishenxisa inkqubo yokuhlanjululwa kwexesha kunye ne-RNA. Itemplate yeRNA inokufumaneka kwimizuzu nje esi-7. Umxube we-5 × ngqo we-RT kunye ne-2 × ngqo qPCR Mix-SYBR reagents ebonelelwe yikiti inokufumana ngokukhawuleza nangokufanelekileyo iziphumo ze-PCR zexesha lokwenyani.
I-5 × i-Direct RT Mix kunye ne-2 × i-Direct QPCR Mix-SYBR ine-inhibitor eyomeleleyo yokunyamezelana, kwaye i-lysate yeesampulu inokusetyenziswa njenge template ye-RT-qPCR ngqo. Le khithi iqulethe i-RNA ekhethekileyo yokuhlangana okuphezulu kwe-Foregene reverse transcriptase, kunye ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.
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Umsila weMouse ngqo kwi-PCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwimisila yegundane, iindlebe, izihlunu kunye nezinye iisampulu zethishu yokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo lwemfuza olukhulu.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65 ° C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwePCR.
I-2 × M1-PCR Easy TM Mix inonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye inokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokufanelekileyo nangokukhulisa okuthile. Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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Imouse Umsila ngqo PCR Kit-UNG
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwimisila yegundane, iindlebe, izihlunu kunye nezinye iisampulu zethishu yokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo lwemfuza olukhulu.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65 ° C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwePCR.
2 × M1-PCR Easy TM Mix (UNG) isebenzisa i-DUTP endaweni ye-DTTP kwisiseko se-2 × M1-PCR Easy TM Mix (UNG), kwaye yongeza i-enzyme ye-UNG (Uracil-N-glycosylase) enokuthotywa itemplate ene-DUTP xeshanye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthoba imveliso yePCR equlathe uracil. I-enzyme ye-UNG ayizukuchaphazeleka kwitemplate engenayo i-uracil, ngaloo ndlela iqinisekisa ukucaciswa kunye nokuchaneka kokukhulisa kunye nokuthintela ukubakho kovavanyo olukhulu lwemfuza. Ukungcoliseka kweemveliso zePCR kwenzeka.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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I-Zebra yeNtlanzi ngqo kwi-PCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwi-zebrafish kunye nezinye izicubu zentlanzi zamanzi amatsha, amaphiko omsila okanye iisampulu zamaqanda entlanzi kwiimpendulo ze-PCR, ke kufanelekile ngokukodwa kuvavanyo lwemfuza olukhulu. Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65 ° C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwePCR.
Umxube we-2 × we-PCR Easy TM unonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye unokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokufanelekileyo nangokukhulisa okuthile. Le reagent iqulethe ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. Isetyenziswe ngokudibeneyo ne-lysis buffer inokufumana ngokukhawuleza iisampulu ngokukhawuleza, kwaye ineempawu zobuntununtunu obuphezulu, ukucaciswa okunamandla, kunye nozinzo olulungileyo.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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I-Zebra yeNtlanzi ngqo kwi-PCR Kit-UNG
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwi-zebrafish kunye nezinye izicubu zentlanzi zamanzi amatsha, amaphiko omsila okanye iisampulu zamaqanda entlanzi kwiimpendulo ze-PCR, ke kufanelekile ngokukodwa kuvavanyo lwemfuza olukhulu. Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65 ° C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwePCR.
2 × PCR Easy TM Mix (UNG) isebenzisa i-DUTP endaweni ye-DTTP kwisiseko se-2 × PCR Easy TM Mix, kwaye yongeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) enokuthomalalisa itemplate ene-DUTP kwangaxeshanye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthoba imveliso yePCR equlathe uracil. I-enzyme ye-UNG ayizukuchaphazeleka kwitemplate engenayo i-uracil, ngaloo ndlela iqinisekisa ukucaciswa kunye nokuchaneka kokukhulisa kunye nokuthintela ukubakho kovavanyo olukhulu lwemfuza. Ukungcoliseka kweemveliso zePCR kwenzeka.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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Tyalela igqabi ngokuthe ngqo kwikhithi yePCR Plus
Le mveliso isebenzisa inkqubo ye-lysis buffer eyahlukileyo yokwenza amagqabi ezityalo. I-lysate inokusetyenziswa njengetemplate ngaphandle kokuhlanjululwa. Emva kokongeza ii-primers, i-PCR Mix yale kit ingasetyenziselwa ukukhulisa.
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Tyala iMbewu ngqo kwiPCR Kit I / II
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer yokufaka imbewu yezityalo. I-lysate inokusetyenziswa njengetemplate ngaphandle kokuhlanjululwa. Emva kokongeza ii-primers, i-PCR Mix yale kit ingasetyenziselwa ukukhulisa.
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Tyala igqabi ngqo PCR kunye nekhithi-UNG
Ngokusekwe kwikhithi ye-Plant Leaf ngqo ye-PCR Plus, i-DUTP iyasetyenziswa endaweni ye-dTTP, kunye ne-enzyme ye-UNG enokuthomalalisa itemplate ene-DUTP. Ngale ndlela, ukucaciswa kunye nokuchaneka kokukhulisa kuyaqinisekiswa, kwaye ingxaki yongcoliseko lwemveliso yePCR enokuthi yenzeke ngexesha lovavanyo olukhulu lwemfuza kuthintelwe.
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Tyalela igqabi ngqo kwi-PCR kit-UNG
Ngokusekwe kwikhithi ye-Plant Leaf ngqo ye-PCR, i-DUTP iyasetyenziswa endaweni ye-dTTP, kunye ne-enzyme ye-UNG enokuthomalalisa itemplate ene-DUTP. Ngale ndlela, ukucaciswa kunye nokuchaneka kokukhulisa kuyaqinisekiswa, kwaye ingxaki yongcoliseko lwemveliso yePCR enokuthi yenzeke ngexesha lovavanyo olukhulu lwemfuza kuthintelwe.
