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Izilwanyana zizonke i-RNA Isolate Kit
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu we-RNA iyonke kwizicwili ezahlukeneyo zezilwanyana. Inika ikholamu yokucoca i-DNA enokususa ngokulula i-genomic DNA kwi-supernatant kunye ne-lysate yethishu. Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Inkqubo yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. IBuffer RW1 kunye neBuffer RW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotein, iDNA, ion, kunye nezinto eziphilayo.
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Iseli iyonke RNA yokuzahlula isixhobo
Le khithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu we-RNA iyonke kwiiseli ezikhule kwi-96, 24, 12, kunye neepleyiti ezingama-6. Ikhithi ibonelela ngekholamu efanelekileyo yokuCoca i-DNA, enokuhlula ngokulula i-supernatant kunye ne-lysate yeseli, ukubopha nokususa i-genomic DNA. Umsebenzi ulula kwaye ugcina ixesha; Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA ngefomula eyahlukileyo. Inani elikhulu leesampulu zinokuqwalaselwa ngaxeshanye.
Yonke le nkqubo ayi-RNase-Simahla, ukuze i-RNA ehlanjululweyo ingahlaziswa; i-Buffer RW1, i-Buffer RW2 ye-buffer yenkqubo yokuhlamba i-RNA engenaziprotheyini, i-DNA, ion kunye nongcoliseko lwekhompawundi.
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Intsholongwane ye-DNA RNA yokuKhetha yedwa
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu wentsholongwane ye-DNA kunye ne-RNA kwiisampulu ezinje ngeplasma, i-serum, ulwelo olungena selfowuni, kunye nenkcubeko yeseli engaphaya kwamandla. Ikhithi yongeza ngokuthe ngqo i-Linear Acrylamide, enokuthi ngokulula ibambe isixa esincinci se-DNA kunye ne-RNA kwiisampulu. I-DNA / RNA-Ikholamu kuphela enokubopha ngokufanelekileyo iDNA kunye neRNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Ikhithi yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. IBuffer RW1 kunye neBuffer RW2 inokuqinisekisa ukuba ifumanekile intsholongwane ye-acid engenayo iprotein, nuclease okanye ezinye izinto ezingcolileyo, ezinokusetyenziselwa ngokuthe ngqo kuvavanyo lwebhayoloji.
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Utyalo lulonke RNA Isolate Plus Kit
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho opheleleyo we-RNA kwizicubu zezityalo ezahlukeneyo ezine-polysaccharides ephezulu okanye umxholo we-polyphenols. Inika ikholamu yokucoca i-DNA enokususa ngokulula i-genomic DNA kwi-supernatant kunye ne-lysate yethishu. Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Ikhithi yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotein, iDNA, ion, kunye nezinto eziphilayo.
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Intsholongwane yeDNA kunye neRNA Kit yokuKhetha
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu wentsholongwane ye-DNA kunye ne-RNA kwiisampulu ezinje ngeplasma, i-serum, ulwelo olungena selfowuni, kunye nenkcubeko yeseli engaphaya kwamandla. Ikhithi yongeza ngokuthe ngqo i-Linear Acrylamide, enokuthi ngokulula ibambe isixa esincinci se-DNA kunye ne-RNA kwiisampulu. I-DNA / RNA-Ikholamu kuphela enokubopha ngokufanelekileyo iDNA kunye neRNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Ikhithi yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. IBuffer RW1 kunye neBuffer RW2 inokuqinisekisa ukuba ifumanekile intsholongwane ye-acid engenayo iprotein, nuclease okanye ezinye izinto ezingcolileyo, ezinokusetyenziselwa ngokuthe ngqo kuvavanyo lwebhayoloji.
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Intsholongwane ye-RNA yokuzahlula
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu we-RNA yentsholongwane kwiisampulu ezinje ngeplasma, i-serum, ulwelo olungena-cell, kunye nenkcubeko yeseli engaphezulu. Ikhithi yongeza ngokuthe ngqo i-Linear Acrylamide, enokuthi ngokulula ibambe isixa esincinci seRNA kwiisampulu. Ikholamu ye-RNA-kuphela enokubopha ngokufanelekileyo i-RNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Ikhithi yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. I-Buffer viRW1 kunye ne-Buffer viRW2 inokuqinisekisa ukuba ifumanekile intsholongwane ye-acid engenayo iprotein, nuclease okanye ezinye izinto ezingcolileyo, ezinokusetyenziselwa ngokuthe ngqo kuvavanyo lwebhayoloji.
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Tyala iyonke i-RNA Isolate Kit
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho opheleleyo we-RNA kwizicubu zezityalo ezahlukeneyo ezineepolysaccharides ezisezantsi kunye nomxholo we polyphenols. Kwiisampulu zezityalo ezine-polysaccharides ephezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba kusetyenziswe i-Plant Total RNA Isolate Plus Kit ukufumana iziphumo ezingcono zeRNA. Ikhithi ibonelela ngekholamu yokucoca i-DNA enokususa ngokulula i-genomic DNA kwi-supernatant kunye ne-lysate yethishu. Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Inkqubo yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotein, iDNA, ion, kunye nezinto eziphilayo.
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Tyala iyonke iRNA Isolate Kit Plus
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho opheleleyo we-RNA kwizicubu zezityalo ezahlukeneyo ezine-polysaccharides ephezulu okanye umxholo we-polyphenols. Inika ikholamu yokucoca i-DNA enokususa ngokulula i-genomic DNA kwi-supernatant kunye ne-lysate yethishu. Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Ikhithi yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotein, iDNA, ion, kunye nezinto eziphilayo.
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Utyalo lweDNA yokuQhawula
Le khithi isebenzisa ikholamu ye-DNA kuphela enokubopha ngokuthe ngqo i-DNA, Foregene protease kunye nenkqubo ekhethekileyo ye-buffer, eyenza lula ukucocwa kwe-DNA ye-genomic yezityalo. Umgangatho ophezulu we-genomic DNA unokufumaneka kwisithuba semizuzu engama-30, ethintela ukonakaliswa kwe-genomic DNA.
Inwebu yejeli ye-silica ye-DNA esetyenziswe kwikholamu ejikelezayo zizinto ezintsha ezahlukileyo ze-Foregene, ezinokuthi zibambe ngokuthe ngqo kwaye zibophe ngokukodwa kwi-DNA, kunye nokwandisa ukususwa kwe-RNA, iiprotein ezingcolileyo, ion, polysaccharides, polyphenols kunye nezinye izinto eziphilayo.
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I-Plasmid Mini Kit ngokubanzi
Le mveliso yamkela kuphela ubuchwephesha bekholamu yokuhlanjululwa kwe-DNA kunye nefomula efanelekileyo ye-SDS ye-lysis, enokufumana i-plasmid DNA ekumgangatho ophezulu kwiibhaktheriya ngaphakathi kwemizuzu engama-20.
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Izilwanyana ze-DNA zokwahlula iKhithi
Le khithi isebenzisa ikholamu ye-DNA kuphela enokubopha ngokuthe ngqo i-DNA, Foregene protease kunye nenkqubo ekhethekileyo ye-buffer. Umgangatho ophezulu we-genomic DNA unokukhutshwa kwiiseli ezahlukeneyo kunye nakwizicubu zezilwanyana ngaphakathi kwemizuzu engama-30 ukuya kwengama-50.
Inwebu yejeli ye-silica ye-DNA esetyenziswe kwikholamu ejikelezayo zizinto ezintsha ezizodwa ze-Foregene, ezinokuthi zibambe ngokuthe ngqo kwaye zibophe ngokukodwa kwi-DNA, kunye nokwandisa ukususwa kwe-RNA, iiprotein ezingcolileyo, ion kunye nezinye iikhompawundi eziphilayo kwiiseli. I-5-80μg ekumgangatho ophezulu ye-genomic DNA inokucocwa kwizicubu ze-10-50mg.
