I-ForeSNP Genotyping Kit
Iinkcukacha
Ukhuphiswano lwe-Allele Specific PCR (i-Competitive Allele Specific PCR) ubuchwepheshe luhlobo olutsha lwendlela yokuchwetheza ye-allele.Le ndlela ayifuni ukudibanisa i-probes ethile kwi-SNP nganye kunye ne-inDel, kodwa idinga kuphela izibini ezimbini ze-probes ze-universal ezizodwa ukufezekisa ukuchwetheza ngokuchanekileyo kweesampuli ze-genomic DNA.Ngokuhlalutya ubungakanani kunye nomlinganiselo wesignali ye-fluorescence yokugqibela, i-genotype izimisele ngokuzenzekelayo, kwaye umphumo wokudibanisa uboniswa ngokubonakalayo.Le ndlela inexesha elifutshane lokubona, ixabiso eliphantsi le-reagent, ukuchaneka okuphezulu, kwaye ingasetyenziselwa ukuzala okuncediswayo kwimolekyuli, ukubeka indawo ye-QTL, ukuchongwa kophawu lwemfuzo kunye neminye imifuniselo yebhayoloji yemolekyuli enomthamo omkhulu wesampulu.
Iinkcukacha
5ml, 50ml, 50ml×10, 500ml×20
Amacandelo ekhithi
2× GT EasyTMXuba |
DNase-Free ddH2O |
Imiyalelo |
Iimpawu&izinto eziluncedo
■Ukuchaneka okuphezulu: ukuchwetheza ulawulo olulungileyo, izinga lokuchaneka lingaphezulu kwe-98%.
■ Ixabiso eliphantsi: Akukho mfuneko yokudityaniswa kwenani elikhulu leeprobe ezixabisa kabini ezibhalwe kabini.
■ Inkqubo yePCR ephuculweyo: uzinzo olululo lwesixokelelwano kunye neenkcukacha eziqinileyo zokukhulisa.
■ Inkqubo ye-PCR yokuchasana nokungcoliseka: Inokuphelisa ngokufanelekileyo ungcoliseko lwe-aerosol olubangelwa yimveliso ye-PCR, ngaphandle kokukhathazeka ngokuphazamiseka kokungcoliseka kwendalo kumqondiso we-fluorescent wolawulo olubi, kwaye iqinisekise ukuchaneka kokukhulisa kunye nokuchaneka kokuchwetheza.
Ukusetyenziswa kweKit
Yenzelwe ukusetyenziswa kuvavanyo lwe-genotyping yeesampuli ze-DNA ezisulungekileyo.
Umzekelo
Kulo vavanyo, i-200ng yengqolowa ye-DNA ye-genomic isetyenziswe njenge template ukufumanisa ukuchwetheza kwe-TaGS-D1 gene ehambelana nobunzima bengqolowa phantsi kwe-Foresnp Genotyping Kit.Imodeli yesixhobo yi-BIO-RAD CFX qhagamshela;inani leesampuli yi-21, inani le-NTCs yi-8, kwaye uhlobo ngalunye lolawulo olulungileyo luyi-1.
I-ForeSNP Genotyping Kit