Ngokubanzi iPlasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Inkcazelo yeKit:
Ucoca ngokukhawuleza i-plasmid DNA ekumgangatho ophezulu ukusuka kwiibhaktheriya eziguquliweyo zovavanyo lwesiqhelo lwebhayoloji yemolekyuli njengotshintsho kunye nokwetyisa kwe-enzyme.
◮Susa i-RNA ngaphandle kokongeza i-RNAse
◮Iluncedo-i-centrifugation yenziwa kwindawo yobushushu begumbi
◮Ukukhawuleza-umsebenzi unokugqitywa kwimizuzu engama-20
◮Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
◮Ukucoceka okuphezulu—OD260/280≈1.7-1.9
Iinkcazelo Kit
I-FOREGENE'S nucleic acid extraction kunye neemveliso zechungechunge zokucoca zinokukhutshwa kwaye zihlanjululwe kwizinga eliphezulu le-nucleic acids ezivela kwimithombo eyahlukeneyo kunye neesampuli zamacandelo anzima.Iimveliso ze-DNA extraction series zisekelwe kwi-DNA-Kuphela i-silica membrane ye-silica iikholamu zokuhlanjululwa kunye ne-reagents ye-formula eyodwa enokuthi ihlambulule i-DNA ephezulu kwiisampuli ezahlukeneyo.
I-General Plasmid Mini Kit isebenzisa iteknoloji yekholomu yokucoca i-DNA-Kuphela kunye nefomula ye-SDS ye-lysis esebenzayo yokufumana i-plasmid DNA ephezulu kwiibhaktheriya ngaphakathi kwemizuzu engama-20.I-DNA-Kuphela i-silica gel membrane esetyenziswe kwikholamu ye-spin yinto entsha ekhethekileyo ye-FOREGENE, enokuthi ibhengeze i-DNA ngokufanelekileyo kwaye ngokuthe ngqo, kwaye isuse i-RNA, iiprotheni ezingcolileyo, i-ion kunye nezinye izinto eziphilayo kwiiseli ukuya kwinqanaba elikhulu.Umthamo omkhulu wokubopha i-plasmid DNA yekholomu yokucoca kuphela yi-60μg DNA.Ubungakanani be-plasmid DNA ekhutshwe ngokwenene ihambelana nomthamo wesisombululo sebhaktheriya, inani leekopi ze-plasmid (ikopi ephezulu, ikopi ephantsi), ubunzima, iimeko zenkcubeko kunye nezinye izinto.
Iinkcukacha
| IKit yePlasmid Mini ngokubanzi | |||
| Umxholo weKit | DE-01001 | DE-01002 | DE-01003 |
| 50T | 100T | 250T | |
| Isithinteli S1 | 10ml | 20ml | 50ml |
| Isithinteli S2 | 10ml | 20ml | 50ml |
| Isithinteli S3 | 25ml | 50ml | 125ml |
| Isithinteli PW | 25ml | 50ml | 125ml |
| Buffer WB2 | 15ml | 30ml | 75ml |
| Isithinteli EB | 10ml | 20ml | 50ml |
| Ikholamu yeDNA-Kuphela | 50 | 100 | 250 |
| Imanyuwali | 1 | 1 | 1 |
Iimpawu&izinto eziluncedo
- Akukho ngcoliseko ye-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-plasmid DNA ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuphepha ukungcoliseka kwebhubhoratri nge-RNase yangaphandle.
- Isantya esikhawulezayo: I-Plasmid DNA extraction ingagqitywa kwimizuzu engama-20.
- Ikhuselekile: Akukho kutsalwa kwe-organic reagent efunekayo.
- Umgangatho ophezulu: I-plasmid efunyenwe ngokutsalwa ayinayo i-RNA, ayikho i-RNase kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
Isicelo seKit
I-Plasmid DNA extraction kunye nokuhlanjululwa kweebhaktheriya ze-Gram-negative.
Ukuhamba komsebenzi
Umzobo
Ukugcinwa kunye nobomi beshelufu
Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yifudumeze kwibhafu yamanzi engama-37 ℃ imizuzu eyi-10 ukunyibilikisa imvula, kwaye uyixube phambi kokusetyenziswa.
-
I-Animal Tissue DNA Isolation Kit DNA Extraction ...
-
EndoFree Maxi Plasmid Kit (Spin Column)
-
FFPE DNA Isolation Kit DNA Extraction Purifica...
-
IsiTyalo seDNA Sokwahlulwa kweKit Genomic Plant DNA Purif...
-
I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi...
-
Buccal Swab/FTA Card DNA Isolation Kit Genomic ...
