Ucoca ngokukhawuleza i-plasmid DNA ekumgangatho ophezulu ukusuka kwiibhaktheriya eziguquliweyo zovavanyo lwesiqhelo lwebhayoloji yemolekyuli njengotshintsho kunye nokwetyisa kwe-enzyme.
◮Susa i-RNA ngaphandle kokongeza i-RNAse
◮Iluncedo-i-centrifugation yenziwa kwindawo yobushushu begumbi
◮Ukukhawuleza-umsebenzi unokugqitywa kwimizuzu engama-20
◮Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
◮Ukucoceka okuphezulu—OD260/280≈1.7-1.9