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I-Lnc-RT Heroᵀᴹ I (Nge-gDNase) (Isimaphambili esikhulu se-strand cDNA synthesis evela kwi-lncRNA)
Iqhawe leLnc-RTTM Mna (Nge-gDNase) yinkqubo ekhutshelweyo ebhalelweyo eyenzelwe ngokukodwa i-lncRNA ukususa ngokukhawuleza ukungcola kwe-genomic ye-DNA. Umxube we-5 × gDNase ungasusa ngokukhawuleza i-genome eseleyo kwi-RNA kwi-42 ° C kangangemizuzu emi-2, ukuthintela ngokufanelekileyo ukuphazamiseka kofuzo kwiziphumo ze-qPCR.
I-5 × L-RT IqhaweTM Umxube uqulethe i-Foregene LncRNA Reverse Transcriptase eyenziwe ngokukodwa yi-Foregene, eluhlobo olutsha lwe-reverse transcriptase ngokukodwa kwi-lncRNA kunye nezinye iitemplate ze-RNA ezintsonkothileyo, ezinobungqingili obomeleleyo be-RNA kunye nokusebenza ngokurekhodayo okuphezulu. Inkqubo elungiselelwe ukwenza umlinganiso okhutshelweyo obuyela umva ngokukhawuleza kwaye unokukhuphela ngokulula iitemplate zeRNA ezinomxholo ophezulu we-GC kunye nolwakhiwo lwesekondari oluntsonkothileyo. I-strand yokuqala ye-cDNA synthesis inokugqitywa kwi-42 ° C kwimizuzu eli-15.
