-
Umsila weMouse ngqo kwi-PCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwimisila yegundane, iindlebe, izihlunu kunye nezinye iisampulu zethishu yokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo lwemfuza olukhulu.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65 ° C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwePCR.
I-2 × M1-PCR Easy TM Mix inonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye inokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokufanelekileyo nangokukhulisa okuthile. Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
