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Imouse Tail SuperDirect PCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwimisila yemouse kunye neesampulu zeendlebe zeempuku zokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo olukhulu lwemfuza.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe ngaphakathi kwemizuzu eyi-10 ukuya kwi-30 kubushushu begumbi (20-25 ° C). Akukho shushu sifunekayo, kwaye ezinye iinkqubo ezinje ngeprotein kunye nokususwa kweRNA azifuneki. Ixabiso elikhutshiweyo le-DNA linokusetyenziswa njengeTemplate yokuphendula kwePCR.
I-2 × M-PCR Easy TM Mix inonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye inokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokufanelekileyo nangokukhulisa okuthile. Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
