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Umsila weMouse SuperDirect PCR Kit Direct PCR Lysis Reagent(Umsila weMouse)(yeGenotyping)

Inkcazelo yeKit:

Sebenzisa ngokuthe ngqo umsila wemouse i-lysate njengethempleyithi yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.

Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.

Imfuno yesampulu incinci, thatha nje imbewu enye.

Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.

Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.

amandla angaphambili


Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha

Le khithi isebenzisa inkqubo ye-lysis buffer eyodwa ukukhulula ngokukhawuleza i-DNA ye-genomic ukusuka kwi-mouse nomsila wempuku kunye neesampuli zendlebe ye-mouse ukwenzela ukusabela kwe-PCR, ngoko ke ifaneleke ngokukodwa kuvavanyo olukhulu lwemfuzo.

Inkqubo yokukhulula i-DNA ye-genomic kwi-lysis buffer igqityiwengaphakathi 10-30 imizuzueubushushu begumbi (20-25°C).Akukho ukufudumeza okufunekayo, kwaye ezinye iinkqubo ezifana neprotheni kunye nokususwa kwe-RNA azidingeki.Amanani omkhondo akhutshiweyo we-DNA angasetyenziswa njenge Template ye-PCR reaction.

2×M-PCR EasyTM Umxube unonyamezelo oluqinileyo kwi-PCR reaction inhibitors, kwaye unokusebenzisa i-lysate yesampuli ukuba ivavanywe njengethemplate yokukhulisa ngokufanelekileyo kunye nokucacileyo.Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.

I-D-Taq DNA polymerase yipolymerase ye-DNA ephuhliswe ngokukodwa yi-Foregene ukwenzela ukuphendula ngokuthe ngqo kwe-PCR.I-D-Taq DNA polymerase inonyamezelo oluqinileyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokwandisa ngokufanelekileyo izixa ze-DNA kwiinkqubo ezahlukeneyo zokusabela ezintsonkothileyo, kunye nesantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo kakhulu kwi-Direct PCR reaction.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I

Isithinteli NP

Foregene Protease Plus I

I-6 × i-DNA yokulayisha i-Buffer

Icandelo II

I-2 × M PCR Easy TM Mix

Imiyalelo

Iimpawu&izinto eziluncedo

Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.

■ Imfuno yesampulu incinci, thatha nje imisila yempuku (okanye iindlebe zempuku ezinobubanzi obuyi-2-5mm) ngaphezulu kwe-2mm.

■ Kulula ukuyisebenzisa, inokufakwa i-lysed ngaphandle kokufudumeza ukuze kusebenze i-protease, kwaye imveliso ye-lysed inokuxhomekeka ngokuthe ngqo kwi-PCR reaction.

■ I-Foregene Mouse Umsila I-Genomic DNA Extraction Kit (DE-05211) inokusetyenziselwa ukukhupha i-genomic DNA kumsila owenziweyo wempuku.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: Umsila wempuku, iindlebe zempuku, izihlunu kunye nezinye izihlunu.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

wk-flw-mouse-tail-SUPER-direct-PCR

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer FP inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

I-Reagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa kwi-4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-Reagent 2 × PCR Easy TM Mix, ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ℃ yokugcina ixesha elifutshane (ukusebenzisa kwiintsuku ze-10).


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