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  • Yintoni umahluko phakathi kwe-PCR, RT-PCR, qPCR, kunye ne-RT-qPCR

    Yintoni umahluko phakathi kwe-PCR, RT-PCR, qPCR, kunye ne-RT-qPCR

    I-PCR yindlela esetyenziselwa ukukhulisa i-DNA kwixabiso elincinci le-template ye-DNA.I-RT-PCR isebenzisa i-reverse transcription ukuvelisa itemplate ye-DNA kumthombo we-RNA onokunyuswa.I-PCR kunye ne-RT-PCR ngokuqhelekileyo ziimpendulo zesiphelo, ngelixa i-qPCR kunye ne-RT-qPCR zisebenzisa i-kinetics yomlinganiselo we-synt yemveliso...
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  • Umphambili wokuFumana iziyobisi: UkuJonga i-RNA ngeeMolekyu eziNcinci

    Umphambili wokuFumana iziyobisi: UkuJonga i-RNA ngeeMolekyu eziNcinci

    Ugonyo lwe-Pfizer lwe-mRNA lwe-COVID lulawule ukuthanda ukusebenzisa i-ribonucleic acid (RNA) njengento ekujoliswe kuyo kunyango.Nangona kunjalo, ukujolisa kwi-RNA ngeemolekyuli ezincinci kulucelomngeni olukhulu.I-RNA ineebhloko zokwakha ezine kuphela: i-adenine (A), i-cytosine (C), i-guanine (G), kunye ne-uracil (U) ethatha indawo ye-thymin...
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  • Yintoni i-nucleic acid amachiza?

    Yintoni i-nucleic acid amachiza?

    “Iziyobisi zeNucleic acid” zisebenzisa i-“nucleic acid,” ebhekisela kwizinto ezinjengeDNA neRNA elawula inkcazelo yemfuza, njengamachiza.Oku kuvumela ukujoliswa kweemolekyuli ezinjenge-mRNA kunye ne-miRNA ezingenakujoliswa kumachiza esintu anobunzima obuphantsi bemolekyuli kunye namachiza e-antibody, kwaye kukho i-g...
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  • Amanye amava malunga ne-siRNA kwi-RT-PCR

    Amanye amava malunga ne-siRNA kwi-RT-PCR

    1. Ulwazi olusisiseko (ukuba ufuna ukubona inxalenye yovavanyo, nceda udlulisele ngokuthe ngqo kwicandelo lesibini) Njengempendulo ephuma kwi-PCR eqhelekileyo, Ixesha langempela i-PCR ibeka iliso kakhulu utshintsho lobungakanani bemveliso yokukhulisa kumjikelo ngamnye we-PCR yokukhulisa. ukusabela ngexesha lokwenyani thr...
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  • RT-PCR yovavanyo lwenkqubo yokusabela indlela yokwenza isishwankathelo esineenkcukacha

    RT-PCR yovavanyo lwenkqubo yokusabela indlela yokwenza isishwankathelo esineenkcukacha

    一、 Yandisa uvakalelo lwenkqubo yokusabela: 1. Yahlula i-RNA ekumgangatho ophezulu: Impumelelo ye-cDNA synthesis ivela kwi-RNA ekumgangatho ophezulu.Umgangatho ophezulu we-RNA kufuneka ubuncinci bube bubude obugcweleyo kwaye ingabinazo ii-reverse transcriptase inhibitors ezifana ne-EDTA okanye i-SDS.Umgangatho we-RNA umisela obona buninzi...
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  • Gqibezela uyilo lweprimer yePCR kunye neenkcukacha zePCR

    Gqibezela uyilo lweprimer yePCR kunye neenkcukacha zePCR

    Isiseko soyilo lwe-Primer (iingxaki ze-99% zingasombululwa) 1. Ubude be-Primer: Incwadi yokufunda idinga i-15-30bp, ngokuqhelekileyo malunga ne-20bp.Ubume bokwenene bungcono ukuba bube yi-18-24bp ukuze kuqinisekiswe ukuchaneka, kodwa ixesha elide elingcono, i-primer ende kakhulu iya kunciphisa ngokukodwa, kwaye inciphise isivuno.2. Prim...
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  • Izilumkiso ze-qRT-PCR ziqulunqwe nguGqirha ophezulu ngobuchule

    Izilumkiso ze-qRT-PCR ziqulunqwe nguGqirha ophezulu ngobuchule

    Wonke umntu uthetha ngomgaqo wovavanyo lwe-qRT-PCR, uyilo lwe-primer, ukutolika kweziphumo, njl., kodwa ndicinga ukuba ndingabelana nawe ngomsebenzi wokulinga we-qRT-PCR.Incinci, kodwa imalunga neziphumo.Ngaphambi kokuba senze i-qRT-PCR, kufuneka sibe nokuqonda okucacileyo kwethu ...
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  • Izilumkiso ze-RT-qPCR

    Izilumkiso ze-RT-qPCR

    Uvavanyo lwe-RT-qPCR lubandakanya ukutsalwa kwe-RNA kunye novavanyo lomgangatho, ukukhutshelwa umva kunye ne-qPCR amanyathelo amathathu, inyathelo ngalinye linezilumkiso ezininzi, siya kwazisa ngokweenkcukacha ngezantsi.Ⅰ.Uvavanyo lomgangatho we-RNA Kuvavanyo lwe-RT-qPCR, emva kokugqitywa kokutsalwa kwe-RNA, ...
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  • Intshayelelo ecokisekileyo kwi-PCR yexesha langempela

    Intshayelelo ecokisekileyo kwi-PCR yexesha langempela

    1. Ukuqonda kokuqala Kweli nqanaba, kufuneka siqonde ezinye iikhonsepthi kunye nesigama, ukuze sigweme ukwenza iimpazamo phambi kwabadala bethu, njengale: Q: Yintoni umahluko phakathi kwe-RT-PCR, qPCR, i-Real-time PCR, kunye nexesha lokwenyani RT-PCR?Impendulo: I-RT-PCR yi-PCR eguqulelwe umva (umva t...
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  • I-PCR, i-PCR eninzi, i-In situ PCR, i-PCR ebuyela umva, i-RT-PCR, i-qPCR (1)-PCR

    I-PCR, i-PCR eninzi, i-In situ PCR, i-PCR ebuyela umva, i-RT-PCR, i-qPCR (1)-PCR

    I-PCR, i-PCR eninzi, i-In situ PCR, i-Reverse PCR, i-RT-PCR, i-qPCR (1)– PCR Siza kulungisa iikhonsepthi, amanyathelo kunye neenkcukacha zee-PCR ezahlukeneyo Ⅰ.I-PCR Polymerase Chain Reaction, ekubhekiselwa kuyo njenge-PCR, bubuchwepheshe bebhayoloji yemolekyuli esetyenziselwa ukukhulisa amaqhekeza athile e-DNA.Ingathathwa njenge...
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  • Umgaqo wovavanyo we-qRT-PCR

    Umgaqo wovavanyo we-qRT-PCR

    I-RT-qPCR iphuhliswe kubuchwepheshe be-PCR obuqhelekileyo.Yongeza iikhemikhali ze-fluorescent (iidayi ze-fluorescent okanye i-fluorescent probes) kwindlela yokusabela ye-PCR yendabuko, kwaye ibone inkqubo yokudibanisa i-PCR kunye nenkqubo yokwandisa ngexesha lokwenyani ngokweendlela zabo ezahlukeneyo zokukhanya.Uphawu lweFluorescent...
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  • RT-PCR yovavanyo lwenkqubo yokusabela indlela yokwenza isishwankathelo esineenkcukacha

    RT-PCR yovavanyo lwenkqubo yokusabela indlela yokwenza isishwankathelo esineenkcukacha

    Ⅰ.Ukwandisa uvakalelo lwenkqubo yokusabela: 1. Ukwahlula-umgangatho ophezulu we-RNA: Impumelelo ye-cDNA synthesis ivela kwi-RNA ephezulu.I-RNA yomgangatho ophezulu kufuneka iqinisekise ubuncinci ixesha elide kwaye ayiqulathanga i-inhibitors engenazo ii-enzymes zokurekhoda, ezifana ne-EDTA okanye i-SDS.Umgangatho...
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