• facebook
  • linkedin
  • youtube

Kwinqanaba lokuqala lokuqhambuka, ngenxa yophuhliso olukhawulezayo, ukuxilongwa ngokukhawuleza kwezigulana ezirhanelwayo sisitshixo sokuthintela i-COVID-19.Ezinye ii-reagents zokubona i-nucleic acid ezivunyiweyo zinexesha elifutshane lophuhliso, kwaye kukho iingxaki ezinjengokuqinisekiswa kokusebenza okukhawulezileyo, ukungonelanga kwe-reagent optimization, kunye nomahluko omkhulu phakathi kweebhetshi;Iingxaki zeelabhoratri ezahlukeneyo zeklinikhi kwimiba eyahlukeneyo yenkqubo yokufumanisa i-nucleic acid zinokuchaphazela ukuchaneka kweziphumo zokufumanisa i-nucleic acid.Eli nqaku liza kugxila kumakhonkco aphambili kunye namanqaku ekubhaqweni kwangoku kwe-SARS-CoV-2 ye-nucleic acid, kunye nokuhlalutya iingxaki zobuxoki obungalunganga kunye novavanyo oluchanekileyo lokufunyanwa kwe-nucleic acid yelebhu kunye nokungahambelani kweklinikhi.

Imigaqo ye-SARS-CoV-2 yokufumanisa i-nucleic acid

I-SARS-CoV-2 yintsholongwane ye-RNA enolandelelwano lwe-genome malunga ne-29 kb, kunye nofuzo olu-10, olunokuthi lufake iiproteni ezili-10 ngempumelelo.Iintsholongwane zenziwe nge-RNA kunye neprotheyini, kwaye umgca ongaphandle yingubo yangaphandle eyenziwe nge-lipids kunye ne-glycoproteins.Ngaphakathi, i-protein capsid isonga i-RNA kuyo, ngaloo ndlela ikhusela i-RNA (P1) eyonakala lula.

zfgd

I-P1 Ulwakhiwo lwe-SARS-COV-2

Iintsholongwane zihlasela iiseli ngokusebenzisa i-cell cell receptors ezithile ukuze zibangele usulelo, kwaye zisebenzise iiseli zehostele ukuphindaphinda.

Umgaqo wokubhaqwa kwe-viral nucleic acid kukuveza i-RNA yentsholongwane nge-cell lysate, kwaye emva koko usebenzise i-real-time fluorescent reverse transcription-polymerase chain reaction (RT-PCR) ukubhaqwa.

Isitshixo somgaqo-siseko wokufumanisa kukusebenzisa iiprimers kunye neeprobes ukuphumeza "ukuhambelana okujoliswe kuyo" kolandelelwano lwe-nucleic acid, oko kukuthi, ukufumana ulandelelwano lwe-nucleic acid ye-SARS-CoV-2 eyahlukileyo kwezinye iintsholongwane malunga neziseko ezingama-30,000 (ukufana kwe-nucleic acid kwezinye iintsholongwane) indawo “ephantsi”), iiprimers kunye neprobe.

Iiprimers kunye neeprobe zihambelana kakhulu nommandla othile we-SARS-CoV-2 nucleic acid, oko kukuthi, ukucaciswa komelele kakhulu.Nje ukuba isiphumo sokwandiswa kwexesha lokwenyani le-RT-PCR yesampulu ivavanywe ilungile, ingqina ukuba i-SARS-CoV-2 ikhona kwisampulu.Jonga iP2.

zfgd2

Amanyathelo e-P2 e-SARS-CoV-2 yokumisela i-nucleic acid (ixesha lokwenyani le-fluorescent RT-PCR)

Iimeko kunye neemfuno zelabhoratri yokufumanisa i-SARS-CoV-2 nucleic acid

Iilabhoratri zovavanyo lwe-Nucleic acid zezona zilungele iimeko zoxinzelelo olubi, kwaye kufuneka zinike ingqwalasela ekujongweni koxinzelelo, zigcine ukuhamba komoya, kwaye ziphelise ii-aerosols.Abasebenzi bovavanyo lwe-Nucleic acid kufuneka babe neziqinisekiso ezihambelanayo, bafumane uqeqesho olufanelekileyo lwe-polymerase chain reaction kwaye baphumelele uvavanyo.Ilabhoratri kufuneka ilawulwe ngokungqongqo, ibekwe kwindawo, kwaye abasebenzi abangabalulekanga bathintelwe ngokungqongqo ukuba bangene.Indawo ecocekileyo kufuneka ifakwe umoya kwaye ibulawe iintsholongwane kwindawo.Izinto ezifanelekileyo zibekwe kwiindawo, zicocekile kwaye zimdaka ziyahlukaniswa, zitshintshwe ngexesha, kwaye zihlanjululwe kwindawo.Ukubulala iintsholongwane ngokwesiqhelo: Isibulali-ntsholongwane esine-chlorine sesona sisombululo siphambili kwiindawo ezinkulu, kwaye i-75% yotywala inokusetyenziswa kwiindawo ezincinci.Indlela efanelekileyo yokujongana ne-aerosols kukuvula iifestile ukuze kungene umoya, kwaye ukubulawa kweentsholongwane emoyeni kunokwenziwa ngokusebenzisa imitha ye-ultraviolet, ukuhluzwa kunye nokubulala iintsholongwane emoyeni.

Amakhonkco aphambili kunye neeparamitha zokumiselwa kwe-SARS-CoV-2 nucleic acid (ixesha lokwenyani le-fluorescent RT-PCR)

Nangona iilebhu ngokubanzi zinika ingqwalasela enkulu kwi-nucleic acid "ukubonwa", enyanisweni, i-nucleic acid "i-extraction" ikwayenye yamanyathelo aphambili okufumana impumelelo, ehambelana ngokusondeleyo nokuqokelela kunye nokugcinwa kweesampulu zentsholongwane.

Okwangoku, iisampuli zokuphefumula ezisetyenziswa kakhulu, ezifana ne-nasopharyngeal swabs, sebenzisa indlela yesibini, isisombululo sokungasebenzi (ukugcinwa) esilungiselelwe ngokusekelwe kwi-nucleic acid extraction kunye nesisombululo se-lysis.Ngakolunye uhlangothi, esi sisombululo sokugcina intsholongwane sinokuyichaza iprotheni yentsholongwane, ilahlekelwe ngumsebenzi wayo kwaye ingasayi kusuleleka, kwaye iphucule ukhuseleko lwenqanaba lokuthutha kunye nokufumanisa;kwelinye icala, inokuqhekeka ngokuthe ngqo intsholongwane ukukhulula i-nucleic acid, isuse i-enzayimu ye-nucleic acid ebolayo, kwaye ithintele intsholongwane.I-RNA ithotyiwe.

Isisombululo sesampulu sentsholongwane esilungiselelwe ngesiseko se-nucleic acid extraction lysis solution.Amacandelo aphambili ziityuwa ezilungeleleneyo, i-ethylenediaminetetraacetic acid chelating agent, i-guanidine ityuwa (guanidine isothiocyanate, iguanidine hydrochloride, njl.), i-anionic surfactant (dodecane) i-sodium sulfate), i-cationic surfactant (tetradecyl methyloxalate, i-hydroxyline, i-hydrochloride, i-hydrochloride), proteinase K kunye namanye amacandelo amaninzi okanye ngaphezulu.Okwangoku, kukho iintlobo ezininzi zeekiti ze-nucleic acid extraction, kunye ne-nucleic acid extraction kunye ne-reagents yokucoca iyasetyenziswa.Nangona i-nucleic acid extraction kunye ne-reagent yokuhlanjululwa isetyenzisiweyo, iinkqubo zokukhutshwa kwekiti nganye ziyahluka.

Okwangoku, iimveliso zekhithi yokubona i-nucleic acid evunyiweyo luLawulo lweeMveliso zoNyango lweSizwe zikhethwa ngokusekwe kwi-ORF1ab, E kunye nofuzo lwe-N kwi-SARS-CoV-2 genome.Imigaqo yokufumanisa iimveliso ezahlukeneyo ziyafana, kodwa iiprimers zazo kunye noyilo lweprobe zahlukile.Kukho amacandelo ekujoliswe kuwo (ORF1ab), amacandelo ekujoliswe kuwo kabini (ORF1ab, N okanye E), kunye namacandelo amathathu ekujoliswe kuwo (ORF1ab, N kunye no-E).Umahluko phakathi kokufunyanwa kunye nokutolika, i-nucleic acid extraction kunye ne-real-time fluorescent RT-PCR inkqubo yokusabela kufuneka ibhekiselele kwimiyalelo yekiti efanelekileyo, kwaye kucetyiswa ukuba abasebenzisi balandele ngokungqongqo indlela yokutolika echazwe kwikiti imiyalelo yokutolika.Imimandla eqhelekileyo, iiprimers kunye nolandelelwano lweprobe olukhuliswe yi-real-time fluorescent RT-PCR iboniswe kwi-P3.

zfgd3

P3 Indawo ye-SARS-CoV-2 amplicon ekujoliswe kuyo kwigenome kunye nolandelelwano lweeprimers kunye neprobes

Ukutolikwa kweziphumo zokumiselwa kwe-SARS-CoV-2 nucleic acid (Rial-Timefluorescent RT-PCR)

“Isicwangciso soThintelo lwePneumonia kunye noLawulo lwe-SARS-CoV-2 usulelo (uHlelo lweSibini)” okwesihlandlo sokuqala sacacisa iindlela zokugweba iziphumo zokwandiswa kwemfuza enye:

1. Akukho Ct okanye Ct≥40 engalunganga;

2. I-Ct <37 ilungile;

3. Ixabiso le-Ct le-37-40 yindawo yegrey-scale.Kucetyiswa ukuba uphinde ulinge.Ukuba isiphumo sokwenza kwakhona i-Ct <40 kunye ne-curve yokukhulisa ineencopho ezicacileyo, isampuli ithathwa njengelungileyo, ngaphandle koko ayilunganga. "

Uhlelo lwesithathu lwesikhokelo kunye noshicilelo lwesine lwesikhokelo luqhubekile nale migaqo ingentla.Nangona kunjalo, ngenxa yeethagethi ezahlukeneyo ezisetyenziswe kwiikiti zorhwebo, ushicilelo lwe-3 olukhankanywe ngasentla lwesikhokelo aluzange lunike imilinganiselo yokumisela ukuhlanganiswa kweethagethi, kugxininisa Imiyalelo enikwe ngumenzi iya kuphumelela.Ukususela kuhlelo lwesihlanu lwezikhokelo, iithagethi ezimbini ziye zacaciswa, ngakumbi iindlela zokugweba kwithagethi enye ekunzima ukuyigweba.Oko kukuthi, ukuba ilabhoratri ifuna ukuqinisekisa ukuba ityala lilungile kubhaqo lwe-nucleic acid ye-SARS-CoV-2, oku kulandelayo kufuneka kuhlangatyezwane ne-1 kwezi-2 zeemeko:

(1) Iithagethi ezimbini ze-SARS-CoV-2 (ORF1ab, N) kwisampulu efanayo zivavanyiwe zinexesha lokwenyani le-fluorescent RT-PCR.Ukuba ithagethi enye ilungile, ukuphinda kuthathwe iisampulu kunye nokuvavanywa kwakhona kuyafuneka.Ukuba ngaba iziphumo zovavanyo zithi Ukuba into ekujoliswe kuyo isenayo, ibonwa njengenayo.

(2) Iisampuli ezimbini zexesha langempela le-fluorescent RT-PCR ibonise injongo enye efanelekileyo ngexesha elinye okanye iisampulu ezimbini zohlobo olufanayo zibonise umphumo wokuvavanya ochanekileyo, onokugwetywa njengento efanelekileyo.Nangona kunjalo, izikhokelo zikwagxininisa ukuba iziphumo ezibi zovavanyo lwe-nucleic acid azinako ukukhuphela ngaphandle usulelo lwe-SARS-CoV-2.Izinto ezinokubangela ukuba izinto ezingalunganga zobuxoki kufuneka zingabandakanywa, kubandakanywa nomgangatho ombi wesampuli (iisampulu zokuphefumula ezivela kwi-oropharynx kunye nezinye iindawo), ukuqokelela isampula ngokukhawuleza okanye emva kwexesha, Iisampuli azizange zigcinwe, zihanjiswe, kwaye ziqhutywe ngokuchanekileyo, kwaye iteknoloji ngokwayo yayineengxaki (i-virus variation, i-PCR inhibition), njl.

Izizathu zobuxoki obungalunganga ekubhaqweni kwe-SARS-CoV-2

Umbono "wobuxoki obungalunganga" kuvavanyo lwe-nucleic acid echaphazelekayo okwangoku, ihlala ibhekisa "kwizinto ezingezizo zobuxoki" apho iziphumo zovavanyo lwe-nucleic acid zingahambelani nokubonakaliswa kweklinikhi, oko kukuthi, iimpawu zeklinikhi kunye neziphumo zokucinga zikrokrelwa kakhulu nge-COVID-19, kodwa iimvavanyo ze-nucleic acid zihlala "zingalunganga" amaxesha amaninzi.Iziko leLebhu yeKlinikhi yeKomishini yezeMpilo yeSizwe yachaza uvavanyo "lwe-SARS-CoV-2" olungeyonyani.

(1) Kukho umlinganiselo othile wentsholongwane kwiiseli zomntu owosulelweyo.Idatha ekhoyo ibonisa ukuba emva kokuba umzimba usulelwe yintsholongwane, intsholongwane ingena emqaleni ngeempumlo kunye nomlomo, emva koko kwi-trachea kunye ne-bronchi, kwaye ifike kwi-alveoli.Umntu osulelekileyo uya kuba nexesha lokufukamela, iimpawu ezithambileyo, kwaye emva koko inkqubo yeempawu ezinzima, kunye nezigaba ezahlukeneyo zesi sifo.Kwaye umthamo wentsholongwane okhoyo kwiindawo ezahlukeneyo zomzimba wahlukile.

Ngokubhekiselele kumthwalo wentsholongwane weentlobo zeeseli, iiseli ze-alveolar epithelial (i-low respiratory tract)> iiseli ze-epithelial zomoya (indlela yokuphefumula ephezulu)> i-fibroblasts, iiseli ze-endothelial, kunye ne-macrophages, njl.;ukusuka kuhlobo lwesampulu, i-alveolar lavage fluid (eyeyona Igqwesileyo)>ukukhohlela nzulu isikhohlela>nasopharyngeal swab>oropharyngeal swab>igazi.Ukongeza, intsholongwane inokubonwa nakwilindle.Nangona kunjalo, xa kucingwa ukuba kube lula utyando kunye nokwamkelwa kwezigulana, isampulu yesampulu esetyenziswa ngokuqhelekileyo yi-oropharyngeal swab>nasopharyngeal swab>bronchial lavage fluid (utyando oluntsonkothileyo) kunye nesikhohlela esinzulu (ukhohlokhohlo olomileyo, kunzima ukulufumana) .

Ngoko ke, inani lentsholongwane kwiiseli ze-oropharynx okanye i-nasopharynx yezinye izigulane zincinci okanye ziphantsi kakhulu.Ukuba kuphela iisampuli ze-oropharynx okanye i-nasopharynx zithathwa ukuze zihlolwe, i-viral nucleic acid ayiyi kufunyanwa.

(2) Akukho ziseli ezinentsholongwane eziqokelelwe ngexesha lokuqokelela isampuli, okanye i-viral nucleic acid ayizange igcinwe ngokufanelekileyo.

[① Indawo yokuqokelela engafanelekanga, umzekelo, xa uqokelela i-oropharyngeal swabs, ubunzulu bokuqokelela akwanelanga, i-swabs ye-nasopharyngeal eqokelelweyo ayiqokelelwa nzulu kwi-nasopharyngeal swabs, njl.

②Iisampulu zesampulu zisetyenziswa ngokungalunganga.Ngokomzekelo, iifayili zokwenziwa ezifana ne-PE fiber, i-polyester fiber kunye ne-polypropylene fiber zinconywa kwizinto eziphathekayo zentloko ye-swab.Iintsinga zendalo ezifana nomqhaphu zisetyenziselwa umsebenzi wokwenene (i-adsorption eqinile yeprotheni kwaye akukho lula ukuyihlamba) kunye ne-nylon fibers (ukungenwa kakubi kwamanzi, okukhokelela kwisampulu enganelanga);

③Ukusetyenziswa ngendlela engafanelekanga kweetyhubhu zokugcina intsholongwane, ezifana nokusetyenziswa kakubi kwepolypropylene okanye i-polyethylene yokugcina iityhubhu zeplastiki ekulula ukufunxa i-nucleic acids (DNA/RNA), okukhokelela ekunciphiseni koxinzelelo lwe-nucleic acid kwisisombululo sokugcina.Ngokwesiqhelo, kuyacetyiswa ukuba kusetyenziswe i-polyethylene-propylene polymer plastic kunye nezinye izikhongozeli zeplastiki ezinepolypropylene eziphathwe ngokukodwa ukugcina i-viral nucleic acids.]

[① Indawo yokuqokelela engafanelekanga, umzekelo, xa uqokelela i-oropharyngeal swabs, ubunzulu bokuqokelela akwanelanga, i-swabs ye-nasopharyngeal eqokelelweyo ayiqokelelwa nzulu kwi-nasopharyngeal swabs, njl.

②Iisampulu zesampulu zisetyenziswa ngokungalunganga.Ngokomzekelo, iifayili zokwenziwa ezifana ne-PE fiber, i-polyester fiber kunye ne-polypropylene fiber zinconywa kwizinto eziphathekayo zentloko ye-swab.Iintsinga zendalo ezifana nomqhaphu zisetyenziselwa umsebenzi wokwenene (i-adsorption eqinile yeprotheni kwaye akukho lula ukuyihlamba) kunye ne-nylon fibers (ukungenwa kakubi kwamanzi, okukhokelela kwisampulu enganelanga);

③Ukusetyenziswa ngendlela engafanelekanga kweetyhubhu zokugcina intsholongwane, ezifana nokusetyenziswa kakubi kwepolypropylene okanye i-polyethylene yokugcina iityhubhu zeplastiki ekulula ukufunxa i-nucleic acids (DNA/RNA), okukhokelela ekunciphiseni koxinzelelo lwe-nucleic acid kwisisombululo sokugcina.Ngokwesiqhelo, kuyacetyiswa ukuba kusetyenziswe i-polyethylene-propylene polymer plastic kunye nezinye izikhongozeli zeplastiki ezinepolypropylene eziphathwe ngokukodwa ukugcina i-viral nucleic acids.]

(4) Umsebenzi welebhu yeklinikhi awufani.Iisampulu zothutho kunye neemeko zokugcina, ukusebenza okusemgangathweni kweelabhoratri zeklinikhi, ukuchazwa kweziphumo kunye nokulawulwa komgangatho zizinto eziphambili zokuqinisekisa ukuchaneka nokuthembeka kweziphumo zovavanyo.Ngokweziphumo zovavanyo lomgangatho wangaphandle olwenziwa yiClinical Laboratory Centre yeKomishoni yezeMpilo yeSizwe ngoMatshi 16-24, 2020, kwiilabhoratri ezingama-844 ezifumene iziphumo ezisebenzayo, i-701 (83.1%) yayifanelekile, kwaye i-143 (16.9%) yayingekho.Ufanelekile, iimeko zovavanyo lwelabhoratri zizonke zilungile, kodwa iilabhoratri ezahlukeneyo zisenomohluko kubuchule bokusebenza kwabasebenzi, isakhono esinye sokutolika esilungileyo, kunye nolawulo lomgangatho.

Ukunciphisa njani ubuxoki obungeyonyani be-SARS-CoV-2 yokufunyanwa kwe-nucleic acid?

Ukunciphisa ama-negatives obuxoki ekufumaneni i-nucleic acid kufuneka kuphuculwe kwiinkalo ezine zokuvelisa izinto ezingalunganga.

(1)Kukho umlinganiselo othile wentsholongwane kwiiseli zomntu owosulelweyo.Ukuxinana kwentsholongwane kwiindawo ezahlukeneyo zomzimba wabantu ekurhaneleka ukuba bosulelekile kuya kwahluka ngamaxesha ahlukeneyo.Ukuba akukho pharynx, isenokuba kwi-bronchial lavage fluid okanye ilindle.Ukuba iintlobo ezininzi zeesampulu zinokuqokelelwa ngexesha elinye okanye kwizigaba ezahlukeneyo zokuqhubela phambili kwesifo kuvavanyo, Kuya kunceda ukuphepha izinto ezingalunganga.

(2) Iiseli eziqulethe intsholongwane kufuneka ziqokelelwe ngexesha lokuqokelela isampuli.Le ngxaki ingasonjululwa kumlinganiselo omkhulu ngokomeleza uqeqesho lwabaqokeleli beesampuli.

(3) Ii-reagents ze-IVD ezithembekileyo.Ngokwenza uphando malunga nokuhlolwa kokusebenza kovavanyo lwee-reagents kwinqanaba likazwelonke, kunye nokuxoxa ngeengxaki ezikhoyo, ukufumanisa ukusebenza kakuhle kwee-reagents kunokuphuculwa ngakumbi kwaye uvakalelo lokuhlalutya lunokuphuculwa.

(4) Ukusebenza okusemgangathweni kweebhubhoratri zeklinikhi.Ngokuqinisa uqeqesho lwabasebenzi basebhubhoratri, ngokuqhubekayo ukuphucula inkqubo yokulawula umgangatho webhubhoratri, ukuqinisekisa ulwahlulo olufanelekileyo, kunye nokuphucula ukukwazi kwabasebenzi ukufumanisa, kunokwenzeka ukunciphisa izinto ezingalunganga zobuxoki ngenxa yemisebenzi engafanelekanga yebhubhoratri.

Izizathu zovavanyo kwakhona lwe-SARS-CoV-2 yovavanyo lwe-nucleic acid kwizigulana ezibuyiselweyo nezikhutshiweyo.

"I-COVID-19 yokuxilongwa kunye neSicwangciso soNyango (uHlelo lweSixhenxe) icacisa ngokucacileyo ukuba enye yeendlela zokunyangwa kwezigulana ze-COVID-19 kwaye zikhutshwe esibhedlele kukuba iisampulu ezimbini ezilandelelanayo zephecana lokuphefumla zinovavanyo olubi lwe-nucleic acid (ubuncinci iiyure ezingama-24 ngokuhlukana), kodwa zimbalwa kakhulu I-SARS-CoV-2 ngenxa yezizathu ezahlukeneyo ze-nucleic acid kwakhona zaphinda zavavanywa ngenxa ye-nucleic acid.

(1)I-SARS-CoV-2 yintsholongwane entsha.Kuyimfuneko ukuqhubela phambili ukuqonda indlela yayo ye-pathogenic, umfanekiso opheleleyo wesifo esibangelwayo kunye neempawu zekhosi yesifo.Ngoko ke, ngakwesinye icala, kuyimfuneko ukuqinisa ulawulo lwezigulane ezikhutshiweyo kunye nokuqhuba ukubonwa kwezonyango iintsuku ezili-14.Ukulandelela, ukubeka iliso kwimpilo kunye nesikhokelo sezempilo ukujulisa ukuqonda kwayo yonke inkqubo yokwenzeka, uphuhliso kunye nesiphumo sesi sifo.

(2)Umguli usenokwasulelwa yintsholongwane kwakhona.Umfundisi-ntsapho uZhong Nanshan uthe: Kuba abaguli abanyangisiweyo bane-antibodies, i-SARS-CoV-2 inokupheliswa zii-antibodies xa ziphinda zihlasela.Zininzi izizathu, ezinokuthi zibe ngunobangela wesigulana esichachayo, okanye sinokunxulumana nokuguquka kwentsholongwane, okanye unobangela wovavanyo lwaselabhoratri.Ukuba yintsholongwane ngokwayo, uguquko lwe-SARS-CoV-2 lunokubangela ukuba i-antibody eveliswe sisigulana esifunyenweyo ukuba ingasebenzi ngokuchasene nentsholongwane etshintshileyo.Ukuba isigulana sosulelwe yintsholongwane eguqulweyo kwakhona, uvavanyo lwe-nucleic acid lunokuthi lube nalo kwakhona.

(3) Ngokubhekiselele kwiindlela zovavanyo lwebhubhoratri, indlela nganye yovavanyo inemida yayo.Ukufunyanwa kwe-SARS-CoV-2 ye-nucleic acid kungenxa yokhetho lolandelelwano lwemfuza, ukubunjwa kwee-reagents, ubuntununtunu bendlela kunye nezinye izizathu, ezikhokelela kwiikhithi ezikhoyo zinemida yazo esezantsi yokufumanisa.Emva kokuba isigulane sinyangwe, intsholongwane emzimbeni iyancipha.Xa umthamo wentsholongwane egazini kwisampulu oza kuvavanywa ungaphantsi komda osezantsi wokufunyanwa, umphumo "ombi" uya kuvela.Nangona kunjalo, esi siphumo asithethi ukuba intsholongwane emzimbeni iphelile ngokupheleleyo.Intsholongwane inokuba semva kokuba unyango luyekiwe.Resurgence”, qhubeka nokukopa.Ngoko ke, kucetyiswa ukuba uphonononge kanye ngeveki kwiiveki ezi-2 ukuya kwezi-4 emva kokukhutshwa.

(4) I-Nucleic acid yimfuza yentsholongwane.Intsholongwane ibulawa emva kokuba isigulana sifumene unyango lwe-antiviral, kodwa amaqhekeza e-RNA entsholongwane eseleyo asagcinwe emzimbeni womntu, kwaye akakhutshwa ngokupheleleyo emzimbeni.Ngamanye amaxesha, phantsi kweemeko ezithile, inokugcinwa ngakumbi.Ixesha elide, kwaye ngeli xesha uvavanyo lwe-nucleic acid luya kuba "ludlulayo" luhle.Ngokwandiswa kwexesha lokubuyisela isigulana, emva kokuba amaqhekeza e-RNA ashiyekileyo emzimbeni aphelile ngokuthe ngcembe, umphumo wovavanyo lwe-nucleic acid unokujika ungabikho.

(5) Iziphumo zovavanyo lwe-nucleic acid ye-SARS-CoV-2 zibonisa kuphela ubukho okanye ukungabikho kwe-RNA yentsholongwane, kwaye ayinakungqina umsebenzi wentsholongwane nokuba intsholongwane iyasulela na.Kuyimfuneko ukubonisa ukuba isigulana esinovavanyo lwe-nucleic acid kwakhona siya kuba ngumthombo wosulelo kwakhona.Kuyimfuneko ukwenza inkcubeko yentsholongwane kwiisampulu zeklinikhi kwaye uhlakulele intsholongwane "ephilayo" ukubonisa ukuba iyosulela.

Isishwankathelo

Ngamafutshane, i-SARS-CoV-2 nucleic acid ivavanya izinto ezingezizo zobuxoki, iziphumo zovavanyo kwakhona, kunye nezinye iimeko ezingahambelaniyo nokubonakaliswa kweklinikhi azinakuthintelwa ngokupheleleyo.Kuvavanyo lokwenyani kunye novavanyo, kuyacetyiswa ukuba udibanise iimpawu zeklinikhi, iimvavanyo zokucinga (CT) kunye novavanyo lweLabhoratri (uvavanyo lwe-nucleic acid + uvavanyo lwe-antibody olukhethekileyo) iziphumo zoxilongo olubanzi lokuthintela ukuxilongwa okuphosiweyo kunye noxilongo olugwenxa.Ukuba iziphumo zovavanyo zifunyaniswa ukuba azihambelani ngokucacileyo kunye nokubonakaliswa kweklinikhi, kuyacetyiswa ukuba kwenziwe uhlalutyo olubanzi lwekhonkco lonke lovavanyo (ukuqokelela isampuli, ukujikeleza kunye namakhonkco okucubungula) ukungabandakanyi intsholongwane ye-SARS-CoV-2 ekuqaleni kosulelo, usulelo oluphindaphindiweyo okanye ludityaniswe nolunye usulelo lwentsholongwane yokuphefumla, njl.Ukuba iimeko ziyavuma, kuyacetyiswa ukuba kuqokelelwe iisampulu ezinovakalelo ngakumbi ezifana nesikhohlela okanye ulwelo lokuhlanjululwa kwe-alveolar ukuze ziphinde zihlolwe.

Iimveliso eziyeleleneyo:

I-SARS-CoV-2 Nucleic Acid yeKit (iMultiplex PCR Fluorescent Probe Method)


Ixesha lokuposa: Sep-03-2021