Kuze kube nguJuni 25, 2021, iKomishoni yezeMpilo yeSizwe yaseTshayina yakhupha idatha ebonisa ukuba abantu abangaphezu kwezigidi ezingama-630 baye bagonywa kwilizwe lam, nto leyo ethetha ukuba izinga lokugonywa kwabo bonke abantu baseTshayina lidlule ngama-40%, elinyathelo elibalulekileyo ekusekeni ukhuseleko lomhlambi.

Abantu abaninzi baya kuba nexhala malunga nokuba bazi njani ukuba baye baphuhlisa amajoni omzimba emva kokufumana isitofu esitsha sokugonya?

Okwangoku, eyona khithi yokufumanisa i-antibody ye-IgM/IgG kwimarike yikiti yokufumanisa i-antibody (indlela yegolide ye-colloidal).

ICoronavirus (COV) lusapho olukhulu lweentsholongwane ezibangela izifo ukusuka kwingqele eqhelekileyo ukuya kwizifo ezinobuzaza ezifana ne-acute acute breathing syndrome (SARS-CoV).I-SARS-CoV-2 luhlobo olutsha olungazange lufunyanwe ebantwini ngaphambili."Isifo seCoronavirus sika-2019" (COVID-19) sibangelwa yintsholongwane "SARS-COV-2" usulelo.Izigulana ze-SARS-CoV-2 zichaze iimpawu ezibuthathaka (kubandakanya ezinye izigulana ezingakhange zixelise iimpawu) ukuba zibi kakhulu.Iimpawu ze-COVID -19 zibonakala njengomkhuhlane, ukudinwa, ukukhohlela okomileyo, ukuphefumla nzima kunye nezinye iimpawu, ezinokukhula ngokukhawuleza zibe yinyumoniya, ukusilela kokuphefumla, ukothuka kwe-septic, ukusilela kwamalungu amaninzi, ukuphazamiseka kwemetabolism ebukhali ye-asidi, njl njl.

Ikhithi entsha yokufumanisa i-IgM/IgG ye-antibody yenzelwe ukufumanisa ngokusemgangathweni izithinteli-ntsholongwane ze-SARS-CoV-2 kwaye ziyisebenzise njengesixhobo esincedisayo ekufumaniseni usulelo lwe-SARS-CoV-2.

Umgaqo wokufumanisa

Ikhithi iqulethe (1) indibaniselwano ye-recombinant neocoronavirus antigen markers and quality control markers protein and (2) imigca emibini yokubhaqa (T1 kunye ne-T2, ngokulandelanayo idityaniswe ne-anti-human IgM kunye ne-IgG antibodies) kunye nomgca wokulawula umgangatho (kubandakanywa Ngu-anti-quality control protein antibody).Xa isampulu yongezwa kuluhlu lovavanyo, iproteni ebhalwe ngegolide ephinda-phinda i-SARS-CoV-2 iya kubophelela kwintsholongwane ye-IgM kunye/okanye i-IgG antibodies ekhoyo kwisampulu ukwenza i-antigen-antibody complex.Ezi zakhiwo zihamba kunye nomgca wovavanyo, kwaye emva koko zibanjwe yi-anti-human antibody IgM kumgca we-T1, kunye / okanye nge-anti-human IgG antibody kumgca we-T2, ibhendi ebomvu-mfusa ibonakala kwindawo yovavanyo, ebonisa umphumo omuhle.Ukuba akukho anti-SRAS-CoV-2 antibody kwisampulu okanye inqanaba le-antibody kwisampulu liphantsi kakhulu, akusayi kubakho migca emfusa-bomvu ku-“T1 kunye ne-T2”."Umgca wokulawula umgangatho" usetyenziselwa ukulawula inkqubo.Ukuba inkqubo yokuvavanya iqhuba ngokuqhelekileyo kwaye i-reagents isebenza ngokufanelekileyo, umgca wokulawula umgangatho kufuneka uhlale ubonakala.

Ii-reagents ezinikezelweyo

Ikhithi nganye iqulethe:

Inkqubo yokufunyanwa

Funda le ncwadana ngononophelo phambi kokuba usebenze ukunqanda iziphumo ezingalunganga.

1. Ngaphambi kokuvavanya, zonke ii-reagents kufuneka zilinganiswe kwiqondo lokushisa kwegumbi (18 ukuya kwi-25 ° C).

2. Thatha ikhadi lovavanyo kwingxowa yefoyile ye-aluminiyam kwaye uyibeke kwindawo ethe tyaba eyomileyo.

3. Inyathelo lokuqala: Sebenzisa i-pipette okanye i-pipette yokudlulisa ukongeza i-10μL ye-serum/plasma, okanye i-20μL yomnwe wegazi elipheleleyo okanye igazi elipheleleyo le-venous kwisampulu yesampulu.

4. Inyathelo lesi-2: Kwangoko yongeza amathontsi ama-2 (60µL) esampula yesithinteli kwisampulu yequla.

5. Inyathelo lesi-3: Xa uvavanyo luqala ukusebenza, unokubona umbala obomvu uhamba kwi-reaction window embindini wekhadi lovavanyo, kwaye umphumo wokuvavanya uya kufumaneka kwimizuzu eyi-10-15..

Ukutolikwa kweziphumo

Okuhle (+)

1. Kukho imigca emi-3 ebomvu (T1, T2, no-C) kwi-reaction window.Nokuba ngowuphi na umgca ovela kuqala, oko kubonisa ubukho be-coronavirus entsha ye-IgM kunye ne-IgG antibodies. 2. Kukho imigca emi-2 ebomvu (i-T1 kunye no-C) kwifestile yokuphendula, kungakhathaliseki ukuba yeyiphi na umgca ovela kuqala, ibonisa ubukho be-coronavirus entsha ye-IgM antibodies. 3. Kukho imigca emibini ebomvu (i-T2 kunye no-C) kwifestile yokuphendula, kungakhathaliseki ukuba yeyiphi na umgca ovela kuqala, ibonisa ubukho be-coronavirus entsha ye-IgG antibodies.

Engalunganga(-)

1. Kuphela umgca "C" (umgca wokulawula umgangatho) kwifestile yokuphendula ubonisa ukuba akukho zithinteli-antibodies kwi-coronavirus entsha zifunyenwe, kwaye isiphumo sibi.

Ayisebenzi

1. Ukuba umgca wokulawula umgangatho (C) awubonakali ngaphakathi kwemizuzu eyi-10-15, umphumo wokuvavanya awuvumelekile kungakhathaliseki ukuba kukho umgca we-T1 kunye / okanye we-T2.Kucetyiswa ukuba uphinde uvavanye. 2. Iziphumo zovavanyo azivumelekanga emva kwemizuzu eli-15.

Ke unokwenza olu vavanyo ekhaya, i-imeyile okanye ufowunele iinkcukacha ezithe vetshe malunga ne-Sars-CoV-2 IgM/IgG yekhithi yokubona i-antibody (indlela yegolide ye-colloidal).