Ukuveliswa kwe-injini yomphunga kuzise uguquko lwezoshishino, ukuqhubela phambili kweekhomputha kudale ihlabathi langoku ledijithali, kwaye inguqu yezobuchwepheshe ngokuqinisekileyo iya kuzisa inkqubela phambili yamaxesha!Ukuzalwa kweISeli Ngqo RT-qPCR KityeForegene iza kuzisa ixesha elitsha lokubhaqwa kweemolekyuli!

7min Fumana itemplate ye-RNA

Ewe, uyive kakuhle, ungathandabuzi, ukusuka kwiseli ukuya kwi-RT-qPCR, ngoku ngamanyathelo amabini kuphela

Yeka ukusabela

Q:Ngaba ukutsalwa kwe-RNA kwanele ngesantya esinjalo?Ngaba kuya kubakho ukosuleleka kweDNA?

A: Sukuba nexhala, iimveliso zethu ziye zathelekiseka ezininzi kunye nokuqinisekiswa, kwaye akukho mahluko xa kuthelekiswa neziphumo zovavanyo lwemveli lwe-RT-qPCR.Ngaphaya koko, ubungakanani besampulu buncinci, kwaye uvavanyo lunokuqhutywa ngobuncinci beeseli ezili-10 ezikhulisiwe.Ingaba i-6, i-12, i-24 okanye i-96-plate-well-plate, iiseli ziyakwazi ukuhlanjululwa ngokuthe ngqo kwimigodi yeplate, elula kwaye ngokukhawuleza.Nge-5 × Direct RT Mix kunye ne-2 × Direct qPCR Mix kwimveliso, ithemplate ye-RNA ingasetyenziselwa ukuguqulwa kwe-high-effective kunye ne-amplification ethile, ngovakalelo oluphezulu, ubungqina obuqinileyo kunye nokuzinza okulungileyo.Ikhithi ye-reverse transcriptase qPCR ilula kakhulu.

ISeli Ngqo RT-qPCR Kitayinayo kuphela inkqubo ye-lysis ephezulu, kodwa iphinde ibe nokuchasana okunamandla kwi-RT-qPCR reaction inhibitors, eyenza yonke ikiti ibe nomda wokufumanisa ophantsi kakhulu, kwaye isenokufumana umphumo omuhle kwiiseli ezili-10. Eli nyathelo elinye le-RT qPCR kit ilula kakhulu.

Ngoku kukho iSYBR eluhlaza qPCR kunye neendlela zeTaqman qPCR ezikhoyo.