Ubume bangoku bentsholongwane yemonkeypox
Ngokutsho kweendaba ze-WHO nge-7 kaJuni, kwiiveki nje ezintathu (ngoMeyi 13 ukuya kuJuni 7), ngaphezu kwe-1,000 yeemeko zemonkeypox ziye zaxelwa kumazwe angama-27 angenayo i-monkeypox-endmic.I-WHO ikholelwa ukuba iimeko zinokujongelwa phantsi ngenxa yolwazi olunyiniweyo lwe-epidemiological kunye nelebhu, kwaye iimeko zingenzeka nakwamanye amazwe.Kodwa le arhente ithe isalugcina uvavanyo "oluphakathi" lwempilo yoluntu jikelele ngokuqhambuka kwemonkeypox.
I-Monkeypox, isifo se-zoonotic esibangelwa yintsholongwane ye-monkeypox (MPXV), ithathwa njengeyona nto ibaluleke kakhulu kwi-orthopoxvirus ethwala izifo kubantu ukususela "ekuphelisweni" kwe-smallpox.Intsholongwane yemonkeypox iyakwazi ukusasazeka ukusuka emntwini ukuya emntwini ngezinto ezigulayo, ulwelo lomzimba, amathontsi okuphefumla, kunye nezinto ezingcolisayo, kodwa le ndlela yosulelo idla ngokufuna ukunxibelelana ngokusondeleyo ixesha elide.
Intsholongwane yeMonkeypox yintsholongwane ye-DNA enemisonto ephindwe kabini enokuthi ikhule kwinkcubeko kwiiseli zezintso zenkawu zaseAfrika, ezibangela iziphumo zecytopathic.Intsholongwane inokufunyanwa ngovavanyo oluninzi: i-enzyme-linked immunosorbent assay (ELISA), uvavanyo lokufumanisa i-antigen, uvavanyo lwe-polymerase chain reaction (PCR), okanye ukuhlukaniswa kwentsholongwane yinkcubeko yeseli.
Umfanekiso ovela kwiwebhusayithi
Silapha ukukunceda ngoqhambuko lwemonkeypox
Njengomthengisi wasekhaya wophando lwebhayoloji yemolekyuli kunye nezisombululo zokuxilongwa kwe-in vitro, i-Foregene Biotech izibophelele ekuncedeni abaphandi bezonyango kunye nenzululwazi ukuba baxilonge kwaye baphande intsholongwane yemonkeypox ngokukhawuleza nangaphezulu.I-Foregene Biotech inobuchwephesha obubophelelayo be-DNA kuphela/i-RNA kuphela kunye nethekhnoloji ye-PCR ethe ngqo, ikubonelela ngeendlela ezahlukeneyo zophando lwezenzululwazi kunye nezixhobo ze-IVD zophando lwakho kunye nokuxilongwa kwentsholongwane yemonkeypox.
Ingcebiso yemveliso yophando lobuNzululwazi#
✿ Ukucocwa ngokufanelekileyo kunye nokukhutshwa kwe-viral DNA kunye ne-RNA
✿ Ukucoceka okuphezulu, akukho ngcoliseko
✿ Akukho DNase kunye ne-RNase ezisetyenziswayo, kwaye imveliso ayithotywanga
✿ Dibana nezicelo ezahlukeneyo zokulinga
✿ Ingasetyenziselwa uhlalutyo lobungakanani betemplates nge-fluorescence quantitative PCR, kwaye ingasetyenziselwa ubhaqo lwe-allele.
✿ Inkqubo eyodwa yokuphucula iPCR ehambelanayo
✿ Ukusebenzisa i-Hot-start Foregene HS Taq Polymerase, ine-amplification ephezulu, uvakalelo kunye neenkcukacha.
#IVD ingcebiso yemveliso#
✿ Isetyenziselwa ukunyangwa kwangaphambili kwesampuli ukuba ihlolwe, ukwenzela ukuba i-nucleic acid ihlolwe kwisampuli ikhutshwe kwimeko yokubopha kunye nezinye izinto, kwaye ingasetyenziselwa iimveliso ze-Foreasy Direct PCR / RT-qPCR ukufezekisa ukufumanisa ngokukhawuleza.
✿ Ukusebenza okulula, isantya sokusebenza ngokukhawuleza, ukuphuma okuphezulu, akukho mfuneko yenkqubo yokukhupha, ukugcina indawo kunye nokuphucula ukusebenza kakuhle
✿ Ifanelekile kwi-PCR ephezulu, ukufunyanwa kwe-PCR ye-multiplex
✿ Ifanelekile kwi-genome template amplification enomxholo ophezulu weGC (> 60%), isakhiwo sesibini esiyinkimbinkimbi kunye nokufumanisa i-genome amplification enkulu
✿ Inomsebenzi oshushu wokuqalisa, ukukhulisa ngokukhawuleza kunye nokuthembeka okuqinileyo
Ixesha lokuposa: Jun-10-2022
