W: Mzalwana, ukukhutshelwa umva kugqityiwe, kwaye ekugqibeleni inyathelo lokugqibela - i-fluorescent quantitative PCR!Hee, mzalwana, yintoni i-PCR yexesha lokwenyani?
M:PCR uhlala uyazi, akunjalo?
W: Uyazi, i-PCR yi-polymerase chain reaction, kwaye iteknoloji eyandisa i-DNA!
M:Kuphantse ukuba… Yindlela yobugcisa ukukhulisa ulandelelwano ekujoliswe kulo ukuya ngaphezu kwesigidi esinye ngokusetyenziswa kweeprayimari ezithile, kodwa oku kubonisa kuphela ubukho okanye ukungabikho kolandelelwano ekujoliswe kulo, kwaye akunakumisela isixa sethemplethi yokuqala.I-Fluorescence quantitative PCR yindlela yobugcisa eyenzelwe ukusombulula le ngxaki.Ixesha lokwenyani iPCR kunye nobuninzi bePCR (qPCR) ibhekisa kuyo ngokubanzi.
W:Umahluko kwi-PCR eqhelekileyo ukwi-fluorescence quantification?
M: Ewe, oko kukuthi, kwinqanaba lokwandiswa komjikelezo ngamnye we-PCR, ubuninzi besignali ye-fluorescence bufunyenwe, kwaye inani le-template yokuqala lingabalwa ngokuhambelana nokutshintsha koxinzelelo lwesignali.
W: Ivakala inzima kakhulu!Ingaba kufuneka ndiyibale ndedwa lento?
M: Oku kufuna ukuba ufunde kancinci, kungcono ukwazi ukuqonda umgaqo.Ngoku unokugxila kwixabiso le-CT kuqala, eyona nto inolwazi lwedatha yovavanyo, kwaye ubungakanani bayo buhambelana nobukhulu bexabiso letemplate yokuqala.Kukwakho neegrafu ezinje ngegophe lokukhulisa kunye namagophe okunyibilika.Banelizwi lokugqibela lokuba ingaba ilinge lakho liphumelele okanye hayi.
W: Oh, bhuti.Emva koko ndiza kwenza umfuniselo we-qPCR!
M: Nika ingqalelo ekhethekileyo ukuba ungafaki iitemplates kwindawo ye-reagent, ngaphandle koko umxube wakho ongasetyenziswanga uya kungcoliseka lula!
W: Ndiyifumene!

Ixesha lokwenyani PCR Easyᵀᴹ-SYBR Green I Kit

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