Imvelaphi
Kwiminyaka yakutshanje, ii-extracellular vesicles (EVs) ziye zatsala umdla wabantu njengesixhobo esinokubakho sonyango;nangona kunjalo, impembelelo yonyango ye-EVs kwi-endometriosis ayizange ixelwe.I-Endometriosis sisifo se-gynecological esinganyangekiyo esichaphazela i-10-15% yabasetyhini abakwiminyaka yokuzala, ebangela iimpawu ezahlukeneyo, okukhokelela ekunciphiseni komgangatho wobomi kunye nomthwalo omkhulu wentlalo.
Intshayelelo yenqaku
Ngomhla wama-20 kaJulayi, 2021, iqela lophando likaNjingalwazi uWang Guoyun ovela kwiSibhedlele saseQilu kwiYunivesithi yaseShandong lapapasha iphepha lophando elinesihloko esithi “M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting Development of Endometriosis” kwiFrontiers in immunology, eyayixoxa ngokuthotywa kwe-M2 macrophages.Ukuba nokwenzeka kwe-nanovesicles (NVs) kunyango lwe-endometriosis.
Eli nqaku lisebenzisa indlela eqhubekayo yokukhupha i-M1NVs, kwaye isebenzisa indlela ye-co-culture ukufunda utshintsho kwi-angiogenesis, ukufuduka, ukuhlasela kunye nezinye izibonakaliso ze-eutopic endometrial stromal cells (EM-ESCs) ezivela kwizigulane ezine-endometriosis.Ngelo xesha, imodeli yegundane ye-endometriosis yasungulwa, kwaye iigundane zaphathwa nge-PBS, i-MONVs okanye i-M1NVs, ngokulandelanayo, ukuvavanya ukusebenza kunye nokhuseleko lwe-M1NV kunyango lwe-endometriosis.
Iziphumo zibonise ukuba i-in vitro M1NVs inokuthintela ngokuthe ngqo okanye ngokungathanga ngqo ukufuduka kunye nokuhlasela kwe-EM-ESCs, kwaye inhibit angiogenesis.Kwimodeli yempuku, i-M1NVs inqanda ukwenzeka kwe-endometriosis nge-M2 macrophage reprogramming ngaphandle kokubangela umonakalo womzimba.Ibonisa ukuba i-M1NVs inokunqanda ngokuthe ngqo ukwenzeka kwe-endometriosis, kwaye ingathintelwa ngokuphinda i-M2 uhlobo lwe-macrophages ukuya kuhlobo lwe-M1.Ke ngoko, ukusetyenziswa kwe-M1NV kunokuba yindlela entsha yonyango lwe-endometriosis.
Uncedo lwangaphambili
Kuphononongo, kuba i-M1NV yalungiswa ngokucofa ngokuqhubekayo i-M1 macrophages, inqaku lisebenzise i-qRT-PCR ukufumanisa izinto eziphazamisayo kunye ne-M1 macrophage markers iNOS, TNF-a kunye ne-IL-6 mRNA kwi-M1NV kunye ne-M1 macrophages.Uphindaphindo olunxulumeneyo lotshintsho.Iziphumo zibonise ukuba i-M1NVs iqulethe i-pro-inflammatory factor mRNA kunye ne-M1 macrophage markers, ebonisa ukuba i-M1NVs inokugcina ngokufanelekileyo iimpawu zokusebenza zeeseli ze-M1.Le ndlela yophando isebenzisa i-QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I yeForegene
ISeli Ngqo RT-qPCR Kitiinkcukacha

Iimeko zesicelo:
 
1. Ukulawulwa kwemfuza kunye nohlalutyo lwentetho, ukuqinisekiswa kwe-gene overexpression okanye umphumo wokuphazamiseka, ukuhlolwa kweziyobisi, njl.;
2. Ukufunyanwa kwembonakalo yemfuza yeeseli ekunzima ukulinywa njengeeseli eziziiprimary, stem cells, kunye nee-nerve cells;
3. Ukufunyanwa kwe-mRNA kwiisampuli ezifana ne-exosomes kunye ne-nanovesicles.
Iimbonakalo: