Ngaphezu kwesiqingatha se-2022, yiza kwaye ubone ukuba yiyiphi amanqaku amanqaku aphezulu athunyelwe nguFoeregene!
01
UKUBA: 17.694
Ipapashwe ngu: The First Affiliated Hospital, Sun Yat-sen University
Umxholo ophambili: I-Esophageal squamous cell carcinoma (ESCC) yeyona nto iphambili yomhlaza wommizo.Ukuchonga iithagethi zonyango ezisebenzayo kubaluleke kakhulu kunyango olungcono lwe-ESCC.Olunye uphononongo lubonise ukuba ukuguqulwa kakubi kwe-RNA kunokukhuthaza intetho kunye nokuguqulelwa kwe-mRNAs enxulumene neseli yomhlaza, kodwa indlela yemolekyuli ayicacanga.Olu pho nonongo lubonise ukuba i-tRNA m7G i-methyltransferase enxulumene neeprotheni eziyinkimbinkimbi ze-METTL1 kunye ne-WDR4 zonyuswe kakhulu kwii-tissue ze-ESCC kwaye zaba negalelo kwi-prognosis embi ye-ESCC.Ukusukela ngoko, izifundo ze-METTL1 kunye ne-WDR4 ezinxulumene ne-gene kwi-ESCC imodeli yeempuku ziqinisekisile ukuba umsebenzi ophuculweyo we-tRNA m7G methyltransferase ukhokelela kulawulo oluphezulu lokubonakaliswa kwezi proteni zimbini, nto leyo ekhuthaza ukwanda kweeseli ze-ESCC.Olu pho nonongo lubonisa umsebenzi obalulekileyo we-oncogenic wokuguqulwa kakubi kwe-tRNA m7G kwi-ESCC kwaye iphakamisa ukuba ukujoliswa kwe-METTL1 kunye neendlela zayo zokubonisa izibonakaliso ezisezantsi kunokuba zithembisa iinjongo zonyango zonyango lwe-ESCC.
Imveliso encediswayo:
Mouse Umsila Ngqo PCR Kit Foregene#TP-01333
Ikhonkco lokuqala:
https://www.nature.com/articles/s41467-022-29125-7
02
UKUBA: 14.903
Ipapashwe ngu: ILabhoratri yeSizwe yoPhando oluPhakathi kwiMithi yaseTshayina, iZiko leSayensi yezoNyango zaseTshayina, kwiYunivesithi yaseMacau
Umxholo ophambili:
Ngophuhliso lwetekhnoloji yokwenziwa, i-mRNA iye yaba ludidi olutsha lwamachiza onyango.Nangona kunjalo, ukuphumeza ukuhanjiswa kwe-intracellular esebenzayo kwamachiza e-mRNA kuhlala kungumngeni ngenxa yobuntununtunu be-mRNA kwii-RNases.Olu phononongo luphakamisa iskimu sokuhanjiswa esifanelekileyo se-polyphenol-conjugated nanostructures enokuhambisa ngokufanelekileyo i-IL-10 mRNA kwi-vivo.Emva kokusebenzisa le nanostructure ukuhambisa iziyobisi ze-mRNA, amanqanaba e-IL-10 aye alawulwa ngokuthe ngqo, avimbela ngokufanelekileyo ukubonakaliswa kwezinto ezivuthayo, ukukhuthaza ukulungiswa kwe-mucosal, ukukhusela iiseli ze-colonic epithelial ukusuka kwi-apoptosis, kunye nokuvimbela i-dextran sodium sulfate-induced acute and chronically inflammatory imodeli ye-murine colitis ephawulekayo inika imodeli entsha ye-colitis.
Imveliso encediswayo:
Animal Total RNA Isolation Kit Foregene#RE-03011
Ikhonkco lokuqala:
03
UKUBA: 12.499
Ipapashwe ngu: ILabhoratri yoMfuniselo weSifo sezilwanyana, iKholeji yoNyango lweZilwanyana, iYunivesithi yezoLimo yaseSichuan
Umxholo ophambili:
Uxinzelelo lwe-oxidative yesidoda yeyona nto ikhokelela ekungachumi kwendoda, ke yinto enokwenzeka nesebenzayo yokuseka iimodeli zezilwanyana zokungazali ngokulungelelanisa inkqubo ye-antioxidant ngendlela ekujoliswe kuyo.Nangona kunjalo, iindlela ze-antioxidant ze-germ cell-specific azikacaciswa.Olu phononongo luqinisekisile ukuba i-LanCL1 gene yi-antioxidant ethile ye-antioxidant kwiiseli zentsholongwane yamadoda, kwaye ukubonakaliswa kwayo kuyancipha okanye kuhambelana nokungazali komntu.Iimpuku eziyindoda ezisweleyo kumfuza we-LanCL1 zibonisa umonakalo we-oxidative yesidoda kunye nokuphazamiseka kokuchuma.Kwinkqubo yokwahlula isidoda kwiiseli zentsholongwane, emva kokuba utyekelo lwe-oxidation lwenzeka, iprotheyini ethile ye-transcription-specific 1 (SP1) iya kulawula ukubonakaliswa kwe-LanCL1 gene, ngaloo ndlela inqanda inkqubo ye-oxidation yesidoda.Olu phononongo lubonisa ukuba indlela ye-SP1-LanCL1 idlala indima ephambili ekulawuleni i-homeostasis yamatyhalarha kunye nokuchuma kwendoda elamlwa yibhalansi ye-redox, kwaye icebisa ukuba iimpuku ezilungisiweyo ze-LanCL1 zinezicelo ezinomtsalane njengemodeli yesilwanyana sobudlolo bamadoda.
Imveliso eNcedayo:
Mouse Umsila DNA Mini Kit Foregene#DE-05211
Ikhonkco loqobo:
http://europepmc.org/article/MED/35469022
◮ Akukho mfuneko yokucoca iDNA ethatha ixesha kwaye ibiza imali eninzi.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.
◮Isistim ye-PCR ephuculweyo yenza ukuba i-PCR ibe nengcaciso ephezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.
Animal Total RNA Isolation Kit
◮Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA.Yonke inkqubo i-RNase-Free.
◮Susa ngokufanelekileyo i-DNA usebenzisa i-DNA-Cleaning Column.
◮ Susa i-DNA ngaphandle kokongeza i-DNase.
◮Ilula—yonke imisebenzi yenziwa kubushushu begumbi.
◮Ukukhawuleza—umsebenzi unokugqitywa ngemizuzu engama-30.
◮Ikhuselekile—akukho sixhobo sokwenza izinto eziphilayo sisetyenzisiweyo.
◮Ucoceko oluphezulu—OD260/280≈1.8-2.1.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Ixesha lokuposa: Sep-13-2022
