Ipapashwe ngu: CAS ILabhoratri engundoqo yePathogen Microbiology kunye ne-Immunology, iZiko leMicrobiology, iAkhademi yaseTshayina yeSayensi
UKUBA: 69.504
Imveliso eNcedayo:ISeli iyonke yeRNA Isolation Kit
Ikhonkco loqobo:https://www.nature.com/articles/s41586-022-05271-2
Usulelo oluneentsholongwane ze-filovirus ezifana ne-Ebola virus (EBOV) kunye ne-Marburg virus zinokubangela iimpawu zeklinikhi ezinzulu, kuquka umkhuhlane we-haemorrhagic kunye nokusilela kwamalungu amaninzi.4.Apha sinqume ubume be-Ebola virus ye-L protein kwi-complex kunye ne-tetrameric VP35 usebenzisa i-cryo-electron microscopy (i-state 1).Uhlalutyo lwesakhiwo lubonise ukuba intsholongwane ye-Ebola L inento yokufaka i-filovirus-specific eyimfuneko kwi-RNA synthesis, kwaye i-VP35 isebenzisana kakhulu nommandla we-N-terminal ye-L ngeeprotomer ezintathu ze-tetramer ye-VP35.
Kweli phepha, i-RNA iyonke yakhutshwa kusetyenziswa i-ForegeneISeli iyonke yeRNA Isolation Kitkwaye ukuxinwa kwe-RNA kwalinganiswa yi-Nanodrop kwi-260 nm, kwaye emva koko yenza ukufumanisa i-RT / qPCR.
■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.
Ixesha lokuposa: Nov-04-2022