Ukwakha i-SOP System

Ukuseka umfuniselo we-PCR we-SOP ukumisela umgangatho wokuziphatha kwabasebenzi bovavanyo.

Abavavanyi bathobela ngokungqongqo iinkqubo zokusebenza, kwaye banciphise ungcoliseko lwePCR olunokuthi lubangelwe zizinto zabantu okanye luthintele ukwenzeka kongcoliseko emsebenzini.Ukongeza, umntu ovavanyayo kufuneka abe nolwazi oluhambelanayo lobuchwephesha kunye nezakhono, kubandakanya nobuchule kwizixhobo ezinxulumene nokusebenza, ukucacisa yonke inkqubo yomsebenzi, ukuqonda iindlela zonyango zongcoliseko kunye neendlela zolawulo lomgangatho welebhu, kunye nokukwazi ukutolika ngokuchanekileyo iziphumo zovavanyo.

Ukwakha iLebhu yePCR esemgangathweni

Ilabhoratri ye-PCR yahlulwe yangamacandelo amane ngokomgaqo, ezizezi, indawo yokulungisa i-reagent, indawo yokusetyenzwa kwesampulu, indawo yokukhulisa, kunye nendawo yohlalutyo lwemveliso.Iindawo ezimbini zokuqala ziindawo zokwandiswa kwangaphambili, kwaye iindawo ezimbini zokugqibela ziindawo ezisemva kolwandiso.Indawo yokwandiswa kwangaphambili kunye nommandla we-post-amplification kufuneka yohlulwe ngokungqongqo.Izinto zokuvavanya, ii-reagents, iphepha lokurekhoda, iipeni, izinto zokucoca, njl njl. zinokuhamba kuphela ukusuka kwindawo yokukhulisa kwangaphambili ukuya kwindawo ye-post-amplification, oko kukuthi, ukusuka kwindawo yokulungisa i-reagent, indawo yokucubungula isampula, indawo yokukhulisa, kunye nommandla wokuhlalutya imveliso yokukhulisa, kwaye akufuneki ihambe kwicala elingasemva.I-airflow kwi-laboratory kufuneka iphinde ihambe ukusuka kwindawo yokwandiswa kwangaphambili ukuya kwindawo ye-post-amplification, kungekhona kwicala elingasemva.

Nciphisa amanyathelo okulinga

Ukuba ilabhoratri ifumanisa kuphela i-PCR kunye nokuchonga, kuyacetyiswa ukuba kusetyenziswe i-fluorescent quantitative PCR endaweni ye-PCR eqhelekileyo.

Fluorescence quantitative PCR iziphumo zokubona zingaqokelelwa kwaye zihlalutywe ngemiqondiso ye-fluorescent, ngoko akukho mfuneko yokuvula isiciko se-electrophoresis emva kokusabela, ethintela ukungcoliswa kweemveliso ze-PCR ezibangelwa ukuvuza kweemveliso zokusabela ukwenza i-aerosols.Ukuba unyusa inani lokuvuleka kwe-cap ngexesha lokulayisha i-gel electrophoresis, ukungcoliswa kwe-aerosol kunokwenzeka.Kucetyiswa ukukhuthaza ukusetyenziswa kwe-PCR yobungakanani kwaye ngokuthe ngcembe ithathe indawo ye-PCR yomgangatho.

Yamkela inkqubo ye-UNG yokuchasana nongcoliseko

Inkqubo yokungcoliseka kwemveliso ye-UNG isetyenziselwa ukusabela kwe-PCR.

Inkqubo isebenzisa i-dUTP endaweni ye-dTTP.Emva kokusabela kwe-PCR, zonke iimveliso ze-PCR (amaqhekeza e-DNA) zifakwe kunye ne-dUTP;kumjikelo olandelayo wokusabela kwe-PCR, i-enzyme ye-UNG eyongeziweyo kwinkqubo ifakwe kwi-37 ° C kwimizuzu ye-5 ngaphambi kwe-PCR, enokuthi ibe yinto ethile yokunciphisa zonke iziqwenga ze-DNA eziqulethe i-dUTP, kwaye emva koko yenze i-PCR reaction.Oku kunokususa ngokupheleleyo ungcoliseko lwe-aerosol olubangelwa ziimveliso zePCR.Isiphumo siboniswe kulo mfanekiso ungezantsi:

Qaphela: Kuluhlu oluthe ngqo lwePCR, ungakhetha uthotho lweemveliso zenkqubo yokungcoliseka kwemveliso ye-PCR ye-FJ Biotech

Cebisa

Kwiilabhoratri ezenza uvavanyo olukhulu lwe-genotyping, kucetyiswa ngamandla ukuba kusetyenziswe inkqubo yokungcoliseka kwemveliso ye-UNG ye-PCR yokuvavanya ii-reagents ukongeza kulwakhiwo lweelabhoratri ezifanelekileyo.

Isikhumbuzo: Usetyenziso lwale nkqubo alunakukususa ungcoliseko lwemveliso yePCR esele yenziwe.Ke ngoko, inkqubo ye-UNG kufuneka isetyenziswe ekuqaleni kovavanyo olufanelekileyo, kwaye inkqubo ye-UNG kufuneka isoloko isetyenziselwa ukukhulisa i-PCR, ukuze kuthintelwe ukungcoliseka kweemveliso ze-PCR.Ubuxoki obuqinisekileyo.

Kuyacetyiswa ukuba usebenzise inkqubo ye-PCR-UNG ye-Forge Biotech xa uqhuba uvavanyo olukhulu, olufana ne:Plant Leaf Direct PCR Kit-UNG;

Imbewu yokutyala ngokuthe ngqo kwi-PCR Kit-UNG;

I-Animal Tissue Direct PCR Kit-UNG;

Mouse Umsila Direct PCR Kit-UNG;

I-Zebra Fish Direct PCR Kit-UNG.

Olu chungechunge lweekiti ezivela kwi-Foregene alukwazi nje ukwenza ukubonwa kwe-PCR ngokukhawuleza kunye nomlinganiselo omkhulu, kodwa kunye nokuthintela ngokufanelekileyo kunye nokulawula ukungcoliswa kwemveliso ye-PCR.