Nokuba ungumntu omtsha kwibhayoloji, okanye ungumphathi omkhulu onezakhono ezomeleleyo kunye namandla, ngaba ukhe wazikhathaza ngokukhupha i-RNA ekumgangatho ophezulu?Bangaphi abantu abachitha iisampulu ezixabisekileyo ngenxa yokusebenza nzima nokunzima kwendlela yeTrizol?

Ngoku, ivangeli iyeza!Yithi ndlelantle ngokupheleleyo kwiindlela zemveli ezitya ixesha kunye nezingasebenziyo, kwaye ukhuphe ngokulula i-RNA ekumgangatho ophezulu kwimizuzu eli-16.

ISeli iyonke yeRNA Isolation Kit

Inkqubo ayinayo i-RNase, akukho mfuneko yokukhutshwa kwe-chloroform, akukho protein kunye nobunye ukungcola, ngoko unokuqiniseka.

Nokuba ungumfundi omtsha omncinci okanye umphathi omkhulu, uya kuba nemigangatho yakho ephezulu yomgangatho wokutsalwa kwe-RNA.

Ke luhlobo luni lwekhithi eyona khithi ifanelekileyo?

Ngamafutshane, lithetha "ngakumbi nangcono."

Akukho ngxaki, yanelisa ukubawa kwakho okuncinci.

Le khithi inokukhupha i-RNA epheleleyo kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates ene-throughput ephezulu.Kukho iintlobo ezininzi ze-RNA efunyenweyo.Ukongeza kwiqhekeza eliqhelekileyo le-28s kunye ne-18s, ii-5 kunye namaqhekeza amancinci e-miRNA nawo anokusetyenziswa.Iqokelelwe.

Ikholamu ekhethekileyo yokucoca i-DNA ikuvumela ukuba ususe i-DNA ye-genomic ngaphandle kokongeza i-DNase, kwaye umphumo ungcono, ukuhlangabezana neemfuno zakho zokulinga.

Ukubona apha, ngaba wonke umntu ususiwe?Ungakhathazeki, imveliso elungileyo, thetha ngeenyani!!!

一, Iluncedo ngakumbi!(16min ayithethi nje)

二、Umgangatho ophezulu!(Isiphumo siyabonakala)

I-Foregene Cell iyonke ye-RNAIsolation Kits Iziphumo zokuthelekiswa kwendlela yekholamu ye-adsorption kunye nendlela ye-Trizol

三, Izinze ngakumbi!Xa sele uyisebenzisile iya kuthi ilungile)

Ipateni ye-Foregene ye-QPCR yejini A emva kokubhalwa kwe-reverse

Iingcebiso:

Xa inani leeseli liphantsi kakhulu, isivuno esipheleleyo se-RNA siya kuncitshiswa, kwaye i-spectrophotometer ayikwazi ukucacisa ngokuchanekileyo ixabiso le-OD.Kuyacetyiswa ukuba kwenziwe uvavanyo lomgangatho ngokusebenzisa iimvavanyo ezilandelayo ze-qPCR.Le mveliso isebenza ngcono kunye ne-RT Easy TM II yenkampani yethu (i-First-strand cDNA synthesis ye-Real Time PCR) (RT-01022)!

Khetha iForegene Cultured Cell Total RNA Extraction Kit ukwenza uvavanyo lwakho lube linyathelo elinye ngokukhawuleza.Ulindele ntoni, yenza ngoku!