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Intshayelelo:

Iimolekyuli ze-RNA zinokusebenzisana ngokubanzi kunye nezinye iimolekyuli zebhayoloji, ezinjengeprotein, iDNA kunye neRNA, kwaye ke zikho kwiiseli kunye nezinto eziphilayo ngendlela ye-macromolecular complexes.Phakathi kwazo, i-RNA-protein complexes bubukho kunye nomsebenzi we-RNA Enye yeendlela ezibalulekileyo.Ukutsalwa kwe-RNA yindlela yakudala yokuhlola kunye nokuchonga iimolekyuli ze-RNA, ngakumbi iiprotheyini ezisebenzisanayo zeemolekyuli ze-lncRNA, kwaye zamkelwa luninzi lwabaphandi kunye neejenali zezemfundo ezigunyazisiweyo zamazwe ngamazwe.Namhlanje i-FOREGENE iya kukwabelana nawe ngomgaqo wovavanyo kunye nenkqubo yobugcisa yokutsalwa kwe-RNA, mhlawumbi ungayisebenzisa.

1. Umgaqo wovavanyo we-RNA wokuwisa itekhnoloji:

I-in vitro transcription kunye ne-synthesis ye-biotin-ileyibhile ekujoliswe kuyo i-lncRNA okanye i-lncRNA amaqhekeza (kubandakanywa amaqhekeza e-RNA asetyenziswa njengolawulo olulungileyo kunye nolubi), kunye nokufukamela ngeencindi zeseli;sebenzisa i-avidin-anchored magnetic beads ukuqokelela i-RNA-protein okanye i-RNA-RNA complexes, Emva kokuhlamba ngokucokisekileyo, i-complex ikhutshwe.Ukuba ufumanisa i-molekyuli ye-protein ejoliswe kuyo esebenzisana ne-lncRNA, yenza i-protein electrophoresis kwi-RNA-tsala imveliso, ibone iprotheni ekujoliswe kuyo nge-staining yesilivere, kwaye unqumle ijeli ye-mass spectrometry;nokwenza ngokuthe ngqo ungqinisiso lwasentshona kwimveliso yokutsalela phantsi;ukuba ichongiwe Kwiimolekyuli ezijoliswe kwi-RNA ezisebenzisana ne-lncRNA, iimveliso ze-RNA-tsala phantsi ziphantsi kwe-RNA extraction kunye nokufunyanwa kwe-qRT-PCR ethile.

2. I-RNA iwisa inkqubo yovavanyo lobuchwephesha:

entsha

3. Inkonzo yobuchwephesha yeRNA yokutsalela phantsi kwe-FOGENE:

Inkcubeko yeseli

Ukulungiswa kweeseli ezipheleleyo / zenyukliya / i-plasma protein extract

I-RNA iwisa umfuniselo

I-SDS-PAGE electrophoresis, ibala lesilivere

I-mass spectrometry kunye nemifuniselo ye-WB

Ingxelo yovavanyo

4. Ityala lovavanyo

Lo mzobo ulandelayo ubonisa iziphumo zokutsalwa kwe-RNA ye-FJ Bio-Bio kunye ne-SDS-PAGE yokubhaqwa kwe-electrophoresis, apho utolo olubomvu luphawula umahluko webhendi:

entsha2

Iqela lophando likaNjingalwazi uSong Xu weYunivesithi yaseSichuan liqokelele iminyaka eyi-15 yokuqokelela kwexesha elide kwicandelo lophando lwe-lncRNA.I-Foregene Bio kunye neqela lophando likaNjingalwazi uSong Xu baye basebenzisana ukusungula itekhnoloji ye-RNA yokuwisa i-lncRNA, eya kukunika isiphumo esanelisayo sovavanyo lwakho.


Ixesha lokuposa: Jun-11-2021