Abathengi beSikolo seSayensi zoBomi, iYunivesithi yaseSichuan yapapasha amaphepha anenqaku eliphezulu kusetyenziswa iimveliso zeForegene, ezinempembelelo ye-17.848

Kutshanje, iqela le-Song Xu eliphuma kwiSikolo seSayensi zoBomi kwiYunivesithi yaseSichuan lipapashe iphepha elinomxholo othi.Imiba ye-Coagulation VII, IX kunye ne-X ziiprotheyini ezilwa ne-antibacterial ngokuchasene ne-Gram-negative Bacteria enganyangekiyo ngamachiza kuPhando lweSeli.

UPhando lweSeli yijenali yezizwe ngezizwe epapashwe ngokudibeneyo yi-Akhademi yaseTshayina yeSayensi kunye neQela loPapasho lweNdalo yaseBritane, enegunya kwilizwe lezemfundo.

Nje ukuba eli nqaku lipapashwe, ngoko nangoko labangela umdla kwizifundiswa.Ukuza kuthi ga ngoku, iziphumo zophando zifunyenwe ngamajelo eendaba amaninzi anje ngeXinhua News Agency, World Wide Web, Phoenix Net, Southern Metropolis Daily,Biological Valley, British Daily Mail, American Daily Science, EurekAlert1!, Springer Nature, Phys.org, njl., I-BioMedCentral kunye namanye amaphephancwadi awaziwayo aneengxelo ezibanzi, kwaye ingqalelo yehlabathi jikelele kwesi siphumo sophando isakhula.

Eli nqaku libonise ukuba izinto ezintathu ze-coagulation VII, IX kunye ne-X ezidlala indima ekuqalisweni kwe-coagulation cascade luhlobo olutsha lweprotheni ye-antibacterial host endogenous, oko kukuthi, i-coagulation factors VII, IX kunye ne-X zinendima ebalulekileyo kwinkqubo yokudibanisa.Isenokukwazi ukulwa ne-Gram-negative bacteria, kuquka ne-"super bacteria" exhathisayo njenge-Pseudomonas aeruginosa kunye ne-Acinetobacter baumannii.

USong Xu, umbhali ohambelana neli nqaku, uthe: “Kwixesha elidlulileyo, bekukholelwa ngokubanzi ukuba izinto ezidityanisiweyo zinokubangela i-thrombosis, kodwa olu phononongo luveze ukuba izinto ezidityanisiweyo nazo zinefuthe elikhethekileyo lokuvala inzala.Oku kufunyanwa okokuqala ekhaya nakwamanye amazwe.”

Imvelaphi yoPhando

Njengoko sonke sisazi, ukunganyangeki kwebhaktiriya kuye kwaba yingxaki enkulu yezempilo yoluntu kwihlabathi liphela.Idatha efanelekileyo ibonisa ukuba phantse i-1 yesigidi sabantu bafa ngenxa yosulelo lwebhaktheriya enganyangekiyo ngamachiza kwihlabathi jikelele minyaka le.Ukuba akukho sicombululo esingcono, inani labantu abafayo nyaka ngamnye ukusuka ngo-2050 liya kuba li-10 lezigidi.

Ukusetyenziswa kakubi kwamayeza okubulala iintsholongwane, kukhatshwa nobuchule obubalaseleyo bokuzivelela kwebhaktiriya, kwenze ukuba ezinye iibhaktheriya ze-pathogenic ezinokuthi zibulawe ngamachiza alwa ne-antibacterial ukuba zinganyangeki ngamachiza, ziphantse zingonakaliseki “super bacteria”.

Ukongeza, xa kuthelekiswa ne-Gram-positive bacteria (Gram+), ibhaktheriya engalunganga (i-Gram-) kunzima ukuyibulala ngenxa yobukho benwebu yangaphandle (icandelo eliphambili yi-LPS, i-alias endotoxin, i-lipopolysaccharide).I-membrane yangaphandle yimvulophu eyenziwe yinwebu yangaphakathi yeseli, udonga olucekethekileyo lweseli kunye nenwebu yangaphandle yeseli.

Imbali yophando

Iqela lika-Song Xu belifunda ngefuthe lezinto ezidityanisiweyo kunyango lwamathumba anobungozi, kodwa ngo-2009, kwafunyaniswa ngokungalindelekanga ukuba izinto ezidityanisiweyo zinokubulala iintsholongwane.Ukuze uchaze indlela ye-bactericidal yezinto ezidibeneyo, iprojekthi sele ineminyaka eyi-10 ukususela ekuqaleni kophando ukuya kupapasho lwephepha.

Ifunyenwe ngengozi

Ngo-2009, abaphandi bafumanisa ukuba i-coagulation factor VII inokulwa ne-Escherichia coli phakathi kwezinto ezingaphezu kweshumi elinesibini.

I-Escherichia coli yeyebhaktiriya e-Gram-negative kwiibhaktheriya.Olu hlobo lwebhaktheriya kunzima ukujongana nalo, kuba iiseli zabo zine-membrane yeseli yangaphakathi, udonga oluncinci lweseli kunye ne-membrane yeseli yangaphandle.Imvulophu inokugcina amayeza ngaphandle kwaye ikhusele ibhaktheriya "ekungeneni."

Cebisa ukucinga

Imiba ye-coagulation liqela leeprotheyini ezisegazini ezibandakanyekayo kwi-blood clotting.Xa ukwenzakala emzimbeni womntu kubangela ukopha, izinto ezahlukahlukeneyo zokujija ziye zisebenze inyathelo nenyathelo ukuze zenze imicu yefibrin, ethi itywine inxeba kunye neeplatelets.Ukuba enye okanye izinto ezininzi zokudityaniswa zinqongophele, ukuphazamiseka kwe-coagulation kuya kwenzeka.

Iingcali zenzululwazi ziye zaqaphela ukuba izigulane ezine-coagulopathy zihlala zithandwa kwizifo zebhaktheriya ezifana ne-sepsis kunye ne-pneumonia.Olu xhulumaniso lwabakhokelela ekubeni bacinge ukuba izinto ezidibeneyo azinakudlala indima ebalulekileyo kuphela kwinkqubo yokudibanisa, kodwa ingaba nefuthe lokuchasana nosulelo.

Uphononongo olunzulu

Ukuze kuphandwe ukuba ngaba izinto ze-coagulation zinokujongana noluhlu olubanzi lweebhaktheriya ze-Gram-negative, abaphandi baqala ukufunda indlela yayo ye-antibacterial nzulu.Bafumanise ukuba i-coagulation factor VII kunye nezinto ezifanayo ngokwesakhiwo i-IX kunye ne-factor X, ezi proteni zintathu zinokugqobhoza kwimvulophu eyomeleleyo yebhaktiriya e-gram-negative.

Uninzi lwezinto ezikhoyo ezilwa ne-antibacterial zijolise kwimetabolism yeseli okanye kwi-membrane zeseli, kodwa ezi zinto zintathu zokudibanisa zineendlela ezahlukeneyo zesenzo.Banokwenza i-hydrolyze LPS, eyona nxalenye iphambili ye-bacterial membrane yangaphandle.Ukulahlekelwa yi-LPS kwenza kube nzima ukuba iibhaktheriya zeGram-negative ziphile.

Hamba uqhubeke

Iqela lophando liye laphonononga ngakumbi indlela kwaye lafumanisa ukubaiprotheyini ye-coagulation factor isebenza kwiibhaktheriya ngokusebenzisa icandelo layo lekhonkco elikhanyayo, ngelixa icandelo lekhonkco elinzima lingenayo i-antibacterial effect.

Kwimeko yendawo yenkcubeko yebhubhoratri, abaphandi baqaphela ngokucacileyo ukuba emva kokongeza i-coagulation factor okanye i-chain chain components, i-envelope ye-bacterial cell envelope yonakaliswa kuqala, kwaye emva kweeyure ze-4, yonke iseli yebhaktheriya yayiphantse yatshatyalaliswa ngokupheleleyo.

Yongeza icandelo lekhonkco lokukhanya le-VII kwi-escherichia coli ekhulisiweyo,

Amacandelo e-membrane yangaphandle yebhaktheriya yonakele, iiseli zitshatyalaliswa

Ayikuphela nje i-Escherichia coli, kodwa ezinye iibhaktheriya ze-Gram-negative ezivavanyiweyo nazo "zoyiswa", kuquka i-Pseudomonas aeruginosa kunye ne-Acinetobacter baumannii.Zombini ezi bhaktiriya zidweliswe nguMbutho wezeMpilo weHlabathi (i-WHO) njengezona bhaktiriya ezili-12 eziyingozi kwimpilo yabantu ngenxa yokunganyangeki kwazo kumachiza.

Ukuqinisekiswa kovavanyo

Olu vavanyo lulandelayo lwezilwanyana luqinisekise ngakumbi ukusebenza kwemiba yokujiyisa ngokuchasene neebhaktheriya ezinkulu.

Abaphandi bafake iigundane ngenani elikhulu le-Pseudomonas aeruginosa enganyangekiyo ngamachiza okanye i-Acinetobacter baumannii.Emva kokutofa iidosi eziphezulu ze-factor VII light chain, iimpuku zasinda;ngelixa iimpuku kwiqela lolawulo zitofwe ngetyuwa eqhelekileyo zazingama-24 Bonke babulawa sisifo emva kweeyure.

Emva kosulelo ngebhaktheriya ephezulu, ukumiliselwa kwe-factor VII light chain

Ingadlala indima yokukhusela kwaye iphucule kakhulu izinga lokusinda kweempuku

Ukubaluleka

Okwangoku, akukho nto ye-antibacterial eyaziwayo ukuba iyasebenza nge-hydrolyzing LPS.

Ukucacisa indlela yokulwa ne-antibacterial esekelwe kwi-LPS hydrolysis kunye neempawu ze-antibacterial of coagulation factor, ezidityaniswe nokukwazi ukuvelisa le miba yokudibanisa kwizinga elikhulu ngexabiso eliphantsi, kunokubonelela ngesicwangciso esitsha esinexabiso eliphantsi lokulwa ne-Gram-negative bacteria enganyangekiyo ngamachiza Ingxaki yezempilo yoluntu engxamisekileyo ibangele.

Ukongeza, lo msebenzi unamathuba amaninzi okusebenza kwimisebenzi yeklinikhi.Okwangoku, akukho machiza aziwayo okulwa neebhaktheriya adlala isiphumo nge-hydrolyzing LPS.Ukudibanisa iipropathi ze-antibacterial ze-FVII, i-FIX, kunye ne-FX ngokuchasene ne-LPS kunye nexabiso eliphantsi lokuvelisa imveliso enkulu, kulindeleke ukuba kuphuhliswe amachiza amatsha ngokuchasene nosulelo "lwe-super bacteria".

Ukwandiswa kwesihloko

Nangona abantu beqhelene kakhulu negama elithi "super bacteria", igama labo elichanekileyo kufuneka libe "ibhaktheriya echasene neziyobisi ezininzi", oku kubhekiselele kuhlobo lweebhaktheriya ezichasene ne-antibiotics ezininzi.

Njengoko bekutshiwo ngaphambili, ukonyuka kokuxhathisa kweebhaktheriya ikakhulu kungenxa yokusetyenziswa ngokungekho ngqiqweni okanye ukusetyenziswa kakubi kwamayeza okubulala iintsholongwane.Ngokomzekelo, ukusetyenziswa kwe-high-frequency ye-antibiotics ebanzi kunyango lwezifo zokuphefumula.

Ukosuleleka komjelo wokuphefumla sisifo sonke esiqhelene naso.Ngokwezibalo, umntwana ngamnye usuleleka malunga ne-6 ukuya kwi-9 ngonyaka, kwaye ulutsha kunye nabantu abadala basuleleka malunga ne-2 ukuya ku-4 ngonyaka.

Ngenxa yokuba izifo zentsholongwane yokuphefumula zivame ukuba ngamasebe angxamisekileyo, ubunzima obukhulu koogqirha bexakeka xa bejongene nezigulane kukuba abanako ukufumana ulwazi lwe-pathogenic ngexesha elifutshane.Ke ngoko, i-lag yeemviwo ze-pathogenic yenza ukuba oogqirha basebenzise i-antibiotics ebanzi (enokuthi isebenze).Ngeentlobo ezininzi zeebhaktheriya).

Yile ndlela “yokusasaza umnatha omkhulu” wamayeza ekhokelele kwingxaki enkulu yokunganyangeki kweyeza yibhaktiriya.Kuba xa uninzi lweentlobo ezinovakalelo luhlala lubulawa, iintlobo ezinganyangekiyo ngamachiza ziyakuphindaphindana ukuze zithathe indawo yeentlobo ezinovakalelo, kwaye izinga lokuxhathisa kwebhaktheriya kwichiza liya kuqhubeka ukunyuka.

Ngoko ke, ukuba ingxelo echanekileyo yokufumanisa i-pathogen inokufumaneka kwixesha elifutshane lokukhokela oogqirha ekuchazeni iyeza elichanekileyo, ukusetyenziswa kwe-antibiotics ebanzi kunokunciphisa kakhulu, ngaloo ndlela kuncitshiswe ingxaki yokuxhatshazwa kwebhaktheriya.

Ejongene nale ngxaki isebenzayo, iqela lophando lwenzululwazi ye-Fuji lamisela ukuphuhlisa ikiti yokufumanisa i-pathogen ye-15-into yokuphefumla.

Le khithi ithatha indibaniselwano ye-PCR ethe ngqo kunye ne-multiplex ye-PCR yobuchwepheshe, enokubona i-Streptococcus pneumoniae, i-methicillin-resistant Staphylococcus aureus, i-Haemophilus influenzae kunye namanye amaphecana ali-15 asezantsi okuphefumla asezantsi kwisikhohlela malunga neyure enye.Iibhaktheriya ze-Pathogenic ziyakwazi ukwahlula ngokufanelekileyo phakathi kweebhaktheriya ze-colonizing (iibhaktheriya eziqhelekileyo) kunye neebhaktheriya ze-pathogenic.Ndiyakholelwa ukuba kuya kulindeleka ukuba ibe sisixhobo esisebenzayo sokunceda abezonyango kusetyenziso lwamayeza oluchanekileyo.

Ebusweni be "super bacteria", intshaba yoluntu lwabantu bonke, uluntu aluzange luthathe lula.Kwinkalo yenzululwazi yobomi, kusekho abaphandi abaninzi abafana neqela lika-Song Xu abasebenza nzima ukuphonononga nokusebenza ngokuthula endleleni ukuze bafumane izisombululo “zebhaktheriya ephezulu”.

Apha, egameni loontanga bebhayoloji kunye nabaxhamli, iFortune Biotech ingathanda ukuvakalisa intlonipho yayo ephezulu kuzo zonke izazinzulu ezizinikele kwimizamo yazo kunye nokubila koku, kwaye ikwathandazela ukuba abantu bangoyise "ibhaktiriya ephezulu" ngokukhawuleza kwaye babe nobomi obukhuselekileyo nobunempilo.ezingqongileyo.