Iindlela ze-PCR kunye nongcoliseko lwe-aerosol ye-nucleic acid kwiilebhu zovavanyo lwe-nucleic acid zifana namacala amabini emali.Sinokukhetha kuphela ukuba nayo okanye ukungabi nayo, kodwa asinakukhetha ukuba siyayifuna okanye siyayichitha.
1. Ukuhlolwa kwe-DNA remover
Ukufezekisa ukususwa kwendawo yokungcoliswa kwe-aerosol ye-nucleic acid, kuyafuneka kuqala ukuba kuhlolwe izisusa ze-DNA ezinokususa i-nucleic acids kwimeko yolwelo.Kuba azikho zininzi izisusi zeDNA ezisebenza ngokwenene.Kwindlela yovavanyo, nceda ubhekisele ku: I-DNA remover ayinakuba "yikona eyimfihlo" yebhubhoratri!
Kolu vavanyo, iikopi ze-100 / μL (CT malunga ne-31) ye-ASFV plasmid kunye ne-DNA remover eqikelelwa yi-PCR yedijithali yaxutywa ngemithamo elinganayo, kwaye emva koko yasabela kwiqondo lobushushu le-10min, i-20min kunye ne-30min ngokulandelelanayo.Emva kokutsalwa kwe-nucleic acid, ukukhulisa i-qPCR kwenziwa.Ulawulo olulungileyo oluxutywe neplasmid kunye namanzi lwathelekiswa.Ekubeni uvavanyo aluzange lugqitywe ngexesha elifanayo, kunokubakho ukutenxa okuthile phakathi kweziphumo, kodwa akuchaphazeli ukugqitywa kovavanyo.Ukuza kuthi ga ngoku, ndivavanye i-10 yeemveliso zokuthengisa ze-DNA.Kuphela uNombolo 1, No.Ezinye iimveliso azinasiphumo.
Itheyibhile 1 Ukususwa kwe-Nucleic acid yesiphumo sokuthengiswa kwe-DNA remover
2. Uvavanyo lwesiphumo se-DNA sokususa iintsholongwane ezisetyenziswa ngokuqhelekileyo
1. Ukuhlolwa kwezibulali-ntsholongwane ezisetyenziswa ngokuqhelekileyo
Zininzi iindidi zezibulali-ntsholongwane ezidla ngokusetyenziswa kwiilabhoratri: iialdehydes, phenols, alcohols, quaternary ammonium salts, peroxides, preparations chlorine, and acids and bases.Iziphumo zokubulala iintsholongwane kwezi zibulali ntsholongwane kwiintsholongwane ziye zaqinisekiswa ngokupheleleyo luvavanyo, kodwa Akukho datha yaneleyo yovavanyo malunga nesiphumo sokuthotywa kwe-nucleic acids.Iilabhoratri zokuvavanya i-Nucleic acid zifuna isibulali-ntsholongwane esingenaso kuphela isiphumo esilungileyo sokubulala iintsholongwane kwi-microorganisms, kodwa sinokuthoba i-DNA.Iziphumo zovavanyo zibonisa ukuba amalungiselelo amabini kuphela eklorini ye-84 ye-disinfectant kunye ne-trichloroisocyanuric acid kunye ne-1 M i-hydrochloric acid ihlangabezana neemfuno.Ekubeni i-1 M i-hydrochloric acid iyingozi kakhulu, kucetyiswa ukuba amalungiselelo e-chlorine asetyenziswe njengenkunkuma yokulahlwa kwe-nucleic acid kwiilebhu zokuvavanya.Nangona kunjalo, amalungiselelo eklorini ayonakalisa isinyithi kwaye ayinakusetyenziselwa ukubulala iintsholongwane kwizixhobo kunye nezixhobo.
Itheyibhile ye-2 imiphumo yokususwa kwe-DNA yeentlobo ezahlukeneyo ze-disinfectants
2. Ubuncinci obusebenzayo bamalungiselelo e-chlorine
Izibulali-ntsholongwane ezisekelwe kwiklorini zinempembelelo eyomeleleyo ye-DNA yokuthotywa, kodwa ngenxa yokubola kwazo zesinyithi kunye nokucaphuka, zingasetyenziselwa ukucoca umgangatho, ii-countertops ezingezizo ezentsimbi, iingcebiso zokumanzisa, iityhubhu ze-centrifuge kunye nezinye izinto zovavanyo.
Ngokutsho kwe "New Coronary Pneumonia Epidemic Disinfection Technical Guidelines": Izinto ezingcolileyo (igazi lezigulane, iimfihlo kunye nokuhlanza) i-chlorine ekhoyo 5g / L-10g / L i-chlorine equkethe isibulala-ntsholongwane;imigangatho, iindonga, kunye nomphezulu wezinto zisebenzisa i-chlorine ekhoyo ye-1g/L Isibulali-ntsholongwane se-Chlorine: iimpahla, ibhedi kunye nezinye izinto ezilukiweyo zifakwa kuqala kwisibulali-ntsholongwane se-chlorine kunye ne-0.5g/L yeklorine ekhoyo kangangemizuzu engama-30, emva koko icocwe njengesiqhelo.
Itheyibhile yesi-3 isiphumo sokususwa kwe-DNA yoxinaniso olwahlukileyo lwe-chlorine disinfectant
Iziphumo zovavanyo zibonisa ukuba: xa ugxininiso olusebenzayo lweklorini lungaphezulu okanye lulingana ne-1.2 g/L, isibulali-ntsholongwane esineklorini sinokuthomalalisa ngokupheleleyo iikopi ezili-100/μL iplasmid xa sisetyenziswa imizuzu emi-5.Xa usebenza imizuzu eli-10, isibulali-ntsholongwane esingama-84 esinogxininiso olusebenzayo lweklorini olungaphezulu okanye olulingana ne-0.6 g/L lunokonakalisa ngokupheleleyo umgangatho weekopi ezili-100/μL zeplasmids.
3. Uvavanyo lokususa umoya olungcoliswe yi-nucleic acid aerosol
Indlela yokucoca umoya ongcoliswe yi-nucleic acid aerosols?Uninzi lweengcinga kukudala indawo engcolisekileyo ye-aerosol kwaye emva koko icoceke, okanye ukucoca i-nucleic acids emva kokungcoliswa.Kum, uvavanyo olungenakuphindwa kwaye iziphumo zovavanyo alunakuhlalutywa ngokobungakanani alunantsingiselo, ngoko ke ndiboleke ezinye iindlela zokubulala iintsholongwane emoyeni.Jonga "i-GB27948-2020 IiMfuno eziBanzi kwii-Disinfectants zoMoya" kunye ne "Disinfection Technical Specifications"
1. Izinto zokuvavanya
1.1 I-DNA remover: Kolu vavanyo, ezimbini ezifumaneka ngokurhweba ukususa i-DNA i-6 kunye ne-8 kwiThebhile 1 zikhethiwe.
1.2 Uvavanyo lwe-plasmid: Ukuqwalasela ukuba ixabiso le-CT longcoliseko oluninzi lwebhubhoratri luya kuba phezulu kune-30, ukuxinwa kwe-ASFV gene plasmid esetyenziswe ngeli xesha malunga neekopi ezili-100/μL, kunye ne-fluorescent quantitative PCR CT imalunga ne-31.07.Yongeza i-plasmid kwiswab yomqhaphu emhlambini kwaye umoya wome.
1.3 Isixhobo sovavanyo: Isixhobo sokubulala iintsholongwane se-Blingkehan KVBOX, esixhaswa ngobubele yi-Shenzhen Runlian Environmental Technology Co., Ltd., inomsebenzi obalaseleyo wokubulala iintsholongwane emoyeni nge-hydrogen peroxide disinfectant, kwaye inokubulala ngokufanelekileyo i-spores kunye nezinye ii-microorganisms.
1.4 Indawo yovavanyo: ifestile yotshintshiselwano evaliweyo malunga ne-0.1 cubic metres.
2. Indlela yokuvavanya
Isixa se-disinfectant esetyenzisiweyo kumatshini we-disinfection yi-10ml, kwaye i-concentration yi-100ml / m3, ephakamileyo kakhulu kune "GB27948-2020 Iimfuno eziPhambili ze-Air Disinfectants": xa usebenzisa i-aerosol spray disinfection, inani le-disinfectant kufuneka libe ≤10 ml / m3
Emva kokutshiza, kwatywinwa iiyure ze-2, kwaye emva koko i-swab emhlambini kunye ne-plasmid yahluthwa nge-TE, kwaye i-swab emhlambini kunye ne-plasmid ngaphandle kwe-fumigation yamiselwa njengolawulo.Sebenzisa i-fluorescence quantitative PCR indlela yokubhaqwa.
Itheyibhile 4 Iziphumo zovavanyo lokususa i-Air nucleic acid
3. Iziphumo zovavanyo
Iintlobo ezimbini ze-DNA zokususa ezinokuthi zithobe ngokufanelekileyo i-plasmids kwimeko yolwelo azinasiphumo konke konke kuvavanyo lokususa i-nucleic acid emoyeni.Ndijonge phambili ekufumaneni isisombululo esingasetyenziselwa ukususa i-nucleic acid i-aerosol pollution emoyeni ukusombulula ngokupheleleyo ingxaki ye-nucleic acid i-aerosol pollution kwii-laboratories zokuvavanya i-nucleic acid, kodwa akukho mveliso ifunyenwe okwangoku.Ndiyakholelwa ukuba kwixesha elizayo, kuya kuba ne-high-performance nucleic acid removers engasetyenziselwa ukukhutshwa kwe-nucleic acid emoyeni.
4. Yeka inkohliso kwaye ugxininise ekuthinteleni
Kungakhathaliseki ukuba iinkampani ziqhayisa njani malunga neemveliso zabo zokususa i-nucleic acid, ndicebisa ukuba uninzi lwabahlobo ababandakanyeka kuvavanyo lwe-nucleic acid banikezela kanye kwaye baqonde inyani: kunzima kakhulu ukususa umoya ongcoliswe yi-nucleic acid aerosols, okanye uthathe umlinganiselo oqinileyo wokuthintela kunye nomsebenzi wokubeka iliso kwimihla ngemihla!
Ixesha lokuposa: Sep-10-2021