MOLWENI, bahlobo!
Emva kokwenza i-nucleic acid extraction iminyaka emininzi kangaka, wonke umntu uyazi kakuhle into yokuba umgangatho we-nucleic acid extraction uyisitshixo kwimpumelelo kunye nokuchaneka kovavanyo olulandelayo.
Amaxesha amaninzi, siyakhathala ukuba umlinganiselo weOD260/OD280 ungaphakathi koluhlu olufanelekileyo.Nje ngale...
Njengoko ilizwi lihamba, awukwazi ukuthetha kakhulu, kwaye awukwazi ukonwaba kwangoko.
Qala ngokumamela uMhleli ekubalisela ibali elincinci!
Iintsomi ezintle ngolo hlobo zihlala zisenzeka kwiilabhoratri ezenza i-nucleic acid extraction.
Ubucinga ukuba uthathe i-RNA kuphela, Uxolo!
Enyanisweni, yinto nje yokuba awazi ukuba uMnumzana Protein uzifihla ngokufihlakeleyo.
Ucinga ukuba ufuna ukwenza uMnumzana Protein abonakale ngeOD260/OD280 enye kuphela?
Umncinci kakhulu!Ilula kakhulu!
Masisebenzise eyona ndlela ibuthathaka yesilivere ukukunceda ubone iiproteni ezifihlwe kwi-RNA!
| Imveliso esetyenzisiweyo | OD260/280 | Misixhobo sokulinganisa |
| I-Foregene | 2.09 | Thermo ScientificTMNanoDropTMNye Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 |
Kudala, kudala, iprotein neRNA yayingabahlobo abasenyongweni.
UMnu Protein uneentloni ngakumbi.
Ke ibisoloko iyi-RNA esebenzayo neyonwabisayo ukudlala ngayo.
Ngolunye usuku.
I-RNA yaxelela uMnumzana iProtein ukuba iza kwamkelwa ngabanye, kwaye uMnumzana Protein wayebuhlungu kakhulu.
I-RNA ikwathandabuza kakhulu kuMnumzana iprotein, kwaye yathi: "Ungathini ukuba ndikubile, uzifihle ezimpahleni zam, uzame ukungabonwa ngabanye, kulungile?"
UMnu. Protein waqhawula uncumo waza wavuma ngovuyo.
Ngomhla wokwamkelwa komntwana, uMnumzana Protein wazifihla kwiimpahla zeRNA kwaye akafunyanwa.Walandela umntu owamkelwayo emva ekhaya waza waphila ubomi obonwabisayo ukususela ngoko.
Ukuthelekisa ukutsalwa kweRNA kunye noForegeneIseliIyonkeI-RNA Extraction Kit
kunye ne-T Company's Reagent ye-Trizol
Sinokubona ngokucacileyo i-banding kwindlela ye-4 (i-Trizol), ngelixa i-lane ye-3 (i-Foregene) icocekile.Iziphumo ziqinisekisile ukuba i-RNA yeprotheyini engcolileyo ekhutshwe yindlela ye-Trizol yayisekho amaconsi amaninzi!Kwaye lane 3 (Forgene) phantse ayinazo iiprotheyini ezingcolileyo kuba umfunisi usebenzise i-Foregene RNA extraction kit!(Intengiso yeza ngokungalindelekanga)
Iinkcukacha ezithe vetshe:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Ixesha lokuposa: Feb-10-2022
