Iindaba zoShishino
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Malunga nama-antibodies e-monoclonal, inqaku elilungileyo ongenakuliphosa!
Ii-antibodies, ezikwabizwa ngokuba yi-immunoglobulins (Ig), ziyi-glycoprotein ezibophelela ngokukodwa kwii-antigens.Ukulungiswa kwe-antibody eqhelekileyo kuveliswa ngokugonywa kwezilwanyana kunye nokuqokelela i-antiserum.Ke ngoko, i-antiserum ihlala iqulethe izilwa-buhlungu ezichasene nezinye ii-antigens ezinganxulumananga kunye nezinye iiproteni c...Funda ngokugqithisileyo -
Iziyobisi ze-Nucleic acid ziye zangena kwixesha legolide, zeziphi iiteknoloji eziphambili ezifuna ukusonjululwa ngokukhawuleza?
Umthombo: I-Medical Micro Emva kokuqhambuka kwe-COVID-19, izitofu ezimbini ze-mRNA zavunywa ngokukhawuleza ukuba zithengiswe, nto leyo ithe yatsala ingqalelo engakumbi kuphuhliso lwamachiza e-nucleic acid.Kwiminyaka yakutshanje, inani lamachiza e-nucleic acid anokuthi abe ngamachiza e-blockbuster apapashe...Funda ngokugqithisileyo -
Uhlalutyo lwezalathisi zoQinisekiso lwe-Primer Probes kwiXesha lokuqala le-PCR Reagents
Ukuqinisekisa ukusebenza kwee-primers kunye ne-probes kwinqanaba lokuqala le-PCR reagents kunye nokumisela ezona meko zifanelekileyo zokusabela ziyimfuneko yokuqinisekisa inkqubela phambili egudileyo yovavanyo olusesikweni.Ke kufuneka siqinisekise njani i-primer probe ekuqaleni ...Funda ngokugqithisileyo -
Amanqaku olwazi lwesampulu ekufuneka abahloli bawabone
Uvavanyo lwaselabhoratri luqala ngokuqokelelwa kwesampulu, kwaye ingqokelela yesampulu yeyona ilula ukuba ingahoywa.Eyona nto ibalulekileyo kuqokelelo lwesampulu kukukhetha uhlobo oluchanekileyo lwesampulu, sebenzisa izixhobo ezifanelekileyo zesampulu, kunye nokuqhuba uthutho olufanelekileyo kunye nokucubungula.I.Isampuli yohlobo oluqhelekileyo sam...Funda ngokugqithisileyo -
Isisombululo esona siphumo songcoliseko lwe-aerosol ye-nucleic acid
Iindlela ze-PCR kunye nongcoliseko lwe-aerosol ye-nucleic acid kwiilebhu zovavanyo lwe-nucleic acid zifana namacala amabini emali.Sinokukhetha kuphela ukuba nayo okanye ukungabi nayo, kodwa asinakukhetha ukuba siyayifuna okanye siyayichitha.1. Ukuhlolwa kwe-DNA remover Ukufezekisa ukususwa kwendawo...Funda ngokugqithisileyo -
Ukuchongwa ngokukhawuleza kwezityalo eziguqukayo
Njengomtsha kwilabhoratri, ayingomsebenzi ulungileyo ukuhluza izityalo eziqinisekileyo ukusuka kwiqela lezityalo ezinezinga eliphantsi loguqulo.Okokuqala, i-DNA kufuneka ikhutshwe kwinani elikhulu leisampulu nganye nganye, kwaye emva koko iijini zangaphandle ziya kubonwa yi-PCR.Nangona kunjalo, iziphumo zihlala zingenanto ...Funda ngokugqithisileyo -
Ukutolikwa ngokubanzi kwamanqaku aphambili ovavanyo lwe-SARS-CoV-2 nucleic acid, kutheni kukho izinto ezingezizo ezingezizo kunye novavanyo kwakhona?
Kwinqanaba lokuqala lokuqhambuka, ngenxa yophuhliso olukhawulezayo, ukuxilongwa ngokukhawuleza kwezigulana ezirhanelwayo sisitshixo sokuthintela i-COVID-19.Ezinye ii-reagents zokubona i-nucleic acid ezivunyiweyo zinexesha elifutshane lophuhliso, kwaye kukho iingxaki ezinjengokuqinisekiswa kokusebenza okukhawulezileyo, ukungonelanga kwerea...Funda ngokugqithisileyo -
Yintoni i-SNP?Izihloko kwiGenetics yabemi
Iileta ezintathu ze-SNP zikho kuyo yonke indawo kuphononongo lwemfuza yabemi.Kungakhathaliseki ukuba uphando lwezifo zabantu, ukubekwa kwezityalo, ukuvela kwezilwanyana kunye ne-ecology ye-molecular, i-SNPs iyadingeka njengesiseko.Nangona kunjalo, ukuba awunako ukuqonda okunzulu kwemfuzo yanamhlanje...Funda ngokugqithisileyo -
Uyiphucula njani intsebenzo ye-LncRNA ereverse transcription?
Iimpawu ze-lncRNA: 1. I-lncRNAs zihlala zinde, zinentetho eguquguqukayo kunye neendlela ezahlukeneyo zokuhlanganisa ngexesha lokwahlula;2. Xa kuthelekiswa nohlobo lwekhowudi, i-lncRNA idla ngokuba ngaphantsi;3. Uninzi lwe-lncRNAs luneenkcukacha ezicacileyo zexesha kunye nesithuba kwinkqubo ...Funda ngokugqithisileyo -
Izisombululo ezine eziphambili zePCR yoLawulo loNgcoliseko lweMveliso
1:Buyisela izixhobo zovavanyo ngexesha Cwangcisa (NTC) ulawulo olungalunganga kwaye uluphinde amaxesha amaninzi.Nje ukuba kufunyaniswe ukuba kukho ungcoliseko lwemveliso yePCR elabhoratri, buyisela zonke izinto zovavanyo ngexesha.Okunje: ukuhlambulula kwakhona kwaye ulungiselele iiprimers, phinda ucofe incam yepipette, E...Funda ngokugqithisileyo -
Ii-enzymes ezimbini ze-RT-PCR ezisebenza kabini
I-traditional reverse transcriptases ayikwazi ukunyamezela ukushisa okuphezulu (ubushushu obuphezulu bomsebenzi we-MMLV yi-37-50 ° C, kunye ne-AMV yi-42-60 ° C).I-RNA yentsholongwane entsonkothileyo ayinakuguqulelwa ngokufanelekileyo kwi-cDNA kumaqondo obushushu aphantsi, okukhokelela ekubhaqweni okusebenzayo Ukuncipha.Tra...Funda ngokugqithisileyo -
Itekhnoloji yokukhulisa i-nucleic acid isothermal
I-PCR yeyona nto isetyenziswa kakhulu itekhnoloji yokukhulisa i-nucleic acid kwaye isetyenziswa kakhulu ngenxa yobuntununtunu kunye nokucaciswa kwayo.Nangona kunjalo, i-PCR idinga ukuphindaphindwa kwe-thermal denaturation kwaye ayinakulahla imida yokuxhomekeka kwizixhobo kunye nezixhobo, ezithintela ukusetyenziswa kwayo kwiklinikhi ...Funda ngokugqithisileyo
