I-China PCR Heroᵀᴹ (NgeDayi) uMvelisi kunye noMboneleli |I-Foregene

PCR Heroᵀᴹ (Ngedayi)

Inkcazelo yeKit:

◮Ukunyaniseka okuphezulu: amaxesha ama-6 e-enzyme yeTaq eqhelekileyo;

◮Isantya sokukhulisa ngokukhawuleza

◮Ukuziqhelanisa ngakumbi kwetemplate

◮Ukusebenza kokwandisa okuphezulu

◮Ukunyamezela kokusingqongileyo kunamandla: kubekwe kwi-37 ° C ngeveki, ukugcina umsebenzi ongaphezu kwe-90%;

◮Ino-5'→3' DNA polymerase umsebenzi kunye 5'→3' exonuclease umsebenzi, ngaphandle 3'→5' umsebenzi exonuclease.

Thumela i-imeyile kuthi Khuphela njengePDF

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha

5ml, 10ml, 40ml

I-PCR Hero (NgeDayi) Ikhithi yokutsalwa ibandakanya isizukulwana esitsha se-Foregene esisodwa se-Taq-Foregene Taq Hero, i-dNTPs ephuculweyo, i-MgCl2, i-reaction buffer, i-PCR reaction enhancer, i-optimizer kunye ne-stabilizer.Inkqubo ye-2 × i-PCR ye-Hero TM (i-dye) inokunyamezela okuphezulu kwi-PCR inhibitors kunenkqubo eqhelekileyo ye-PCR Mix, kwaye inokumelana ngokulula nokukhulisa i-PCR yeetemplates ezahlukeneyo eziyinkimbinkimbi.Inkqubo yokusabela ekhethekileyo kunye ne-Taq Hero ephezulu yenza ukuba i-PCR iphendule ibe nempumelelo ephezulu yokukhulisa, ukuchaneka kunye novakalelo.

Amacandelo ekhithi

0.1U Taq Hero/μl

400μIi-M dNTPs

20mM Tris-HCl(pH8.8)

100mM KCl

5mM MgCl2

Ezinye izizinzi kunye nezixhasi

DNA Ilayisha isithinteli

Iimpawu&izinto eziluncedo

■Ukunyaniseka okuphezulu: amaxesha ama-6 e-enzyme yeTaq eqhelekileyo;

■ Isantya sokukhulisa ngokukhawuleza : isantya sokukhulisa yi-5-10sec/kb, ephindwe ka-6-12 kune-enzyme ye-Taq eqhelekileyo;

■ Ukuguquguquka okuthe kratya kwetemplate: Inokukhulisa ngokufanelekileyo iitemplate ezahlukeneyo ze-DNA ezintsonkothileyo eziphezuluUmxholo weGC;

■ Ubuchule obuphezulu bokwandisa: imijikelo esezantsi yokwandisa kune-enzyme yeTaq eqhelekileyo;

■ Ukunyamezela kokusingqongileyo kunamandla: kubekwe kuma-37°C kangangeveki, ukugcina umsebenzi ongaphezulu kwama-90%;

■ Inezi-5'→3'Umsebenzi we-DNA polymerase kunye ne-5'→3'umsebenzi we-exonuclease, ngaphandle kwe-3'→5'umsebenzi we-exonuclease.

■ Ikiti ixutywe kwangaphambili kunye ne-Electrophoresis Loading Buffer, emva kokuba i-PCR igqityiwe, isampuli inokulayishwa ngokuthe ngqo kwi-electrophoresis;ukusebenza kulula kwaye kulula.