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I-Lnc-RT Heroᵀᴹ I (Nge-gDNase) (Isimaphambili esikhulu se-strand cDNA synthesis evela kwi-lncRNA)
Iqhawe leLnc-RTTM Mna (Nge-gDNase) yinkqubo ekhutshelweyo ebhalelweyo eyenzelwe ngokukodwa i-lncRNA ukususa ngokukhawuleza ukungcola kwe-genomic ye-DNA. Umxube we-5 × gDNase ungasusa ngokukhawuleza i-genome eseleyo kwi-RNA kwi-42 ° C kangangemizuzu emi-2, ukuthintela ngokufanelekileyo ukuphazamiseka kofuzo kwiziphumo ze-qPCR.
I-5 × L-RT IqhaweTM Umxube uqulethe i-Foregene LncRNA Reverse Transcriptase eyenziwe ngokukodwa yi-Foregene, eluhlobo olutsha lwe-reverse transcriptase ngokukodwa kwi-lncRNA kunye nezinye iitemplate ze-RNA ezintsonkothileyo, ezinobungqingili obomeleleyo be-RNA kunye nokusebenza ngokurekhodayo okuphezulu. Inkqubo elungiselelwe ukwenza umlinganiso okhutshelweyo obuyela umva ngokukhawuleza kwaye unokukhuphela ngokulula iitemplate zeRNA ezinomxholo ophezulu we-GC kunye nolwakhiwo lwesekondari oluntsonkothileyo. I-strand yokuqala ye-cDNA synthesis inokugqitywa kwi-42 ° C kwimizuzu eli-15.
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Ixesha Lokwenene PCR Easyᵀᴹ-Taqman
I-2X eyi-PCR yokwenene ilulaTM Umxube-Taqman obonelelwe liXesha leXesha le-PCR elilulaTM-I-Taqman kit yinkqubo entsha ye-premix esebenzisa iinkqubo ezithile ze-fluorescent ze-Real Time PCR zokunyusa iimpendulo, ezinokuphucula kakhulu imveliso ethile kunye nemvakalelo yokuphendula. I-ROX ibonelelwe njengedayi yolawulo lwangaphakathi.
2X yokwenene PCR EasyTM UMix-Taqman uqulethe i-Foregene ekhethekileyo yokuqalisa ukutshisa iTaq DNA Polymerase. Xa kuthelekiswa nee-enzyme eziqhelekileyo zeTaq, inezibonelelo zokukhulisa ukusebenza okuphezulu, amandla okukhulisa okomeleleyo kunye nezinga eliphantsi lokungalingani. Inokunciphisa ukukhulisa okungangqalanga kunye nokuphucula ukuchaneka kwePCR.
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Ixesha Lokwenene PCR Easyᵀᴹ-SYBR Green I kit
I-2X eyi-PCR yokwenene ilulaTM Umxube-SYBR obonelelwe liXesha leXesha le-PCR elilulaTM-SYBR Green I kit yinkqubo entsha yeprimix esebenzisa i-SYBR Green I yeXesha leXesha lokuPhendula kokunyusa i-PCR, enokuphucula kakhulu imveliso ethile kunye nemvakalelo yokuphendula. I-ROX ibonelelwe njengedayi yolawulo lwangaphakathi. Ubungakanani be-fluorescence yale khithi amaxesha angama-3-5 eemveliso ezifanayo, ezinokuba novakalelo ngakumbi kwaye zibonakalise ngokungathandabuzekiyo ukugxila kwethemplate ekujolise kuyo.
2X yokwenene PCR EasyTM Umxube-SYBR uqulethe i-Foregene ekhethekileyo eshushu-yokuqalisa iTaq DNA Polymerase. Xa kuthelekiswa nee-enzyme eziqhelekileyo zeTaq, inezibonelelo zokukhulisa ukusebenza okuphezulu, amandla okukhulisa okomeleleyo kunye nezinga eliphantsi lokungalingani. Inokunciphisa ukukhulisa okungangqalanga kunye nokuphucula ukuchaneka kwePCR.
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PCR Heroᵀᴹ (NgeDayi)
I-2X eyi-PCR yokwenene ilulaTM Umxube-SYBR obonelelwe liXesha leXesha le-PCR elilulaTM-SYBR Green I kit yinkqubo entsha yeprimix esebenzisa i-SYBR Green I yeXesha leXesha lokuPhendula kokunyusa i-PCR, enokuphucula kakhulu imveliso ethile kunye nemvakalelo yokuphendula. I-ROX ibonelelwe njengedayi yolawulo lwangaphakathi. Ubungakanani be-fluorescence yale khithi amaxesha angama-3-5 eemveliso ezifanayo, ezinokuba novakalelo ngakumbi kwaye zibonakalise ngokungathandabuzekiyo ukugxila kwethemplate ekujolise kuyo.
2X yokwenene PCR EasyTM Umxube-SYBR uqulethe i-Foregene ekhethekileyo eshushu-yokuqalisa iTaq DNA Polymerase. Xa kuthelekiswa nee-enzyme eziqhelekileyo zeTaq, inezibonelelo zokukhulisa ukusebenza okuphezulu, amandla okukhulisa okomeleleyo kunye nezinga eliphantsi lokungalingani. Inokunciphisa ukukhulisa okungangqalanga kunye nokuphucula ukuchaneka kwePCR.
