Ikhithi ye-PCR ethe ngqo (ngaphandle kweZixhobo zokuSampula) iProtokholi ethe ngqo kwi-PCR evela kwiMathiriyeli yeSityalo
Iinkcazelo
Le mveliso isebenzisa inkqubo ye-lysis buffer eyodwa yokukhupha amagqabi esityalo.I-lysate ingasetyenziselwa njenge template ngaphandle kokuhlanjululwa.Emva kokongeza i-primers, i-PCR Mix yale khithi ingasetyenziselwa ukukhulisa.
Iinkcukacha
0×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Amacandelo ekhithi
Icandelo I | Isithinteli P1 |
Isithinteli P2 | |
6×DNA Ilayisha isithinteli | |
Icandelo II | 2× Igqabi PCR EasyTMXuba |
Iimpawu&izinto eziluncedo
■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi
■ imathiriyeli encinci
■ I-Simple-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya
■ 2 × Ukuxuba, ukunciphisa iimpazamo zesampulu zokulayisha kunye nexesha lokulungiselela inkqubo yokusabela
■ Ulungiselelo olukhawulezayo lwetemplate lunokugqityezelwa kwimizuzu eli-10, ukusabela kwe-PCR kunokugqityezelwa ubuncinane kwimizuzu engama-50.
■ Ukusabela okuphezulu kwe-throughput-lysis kunokugqitywa kwi-96-well PCR plate
Iiparamitha zeKit
Isicelo: ukuchongwa okuguqulwe ngokwemfuza, i-genotyping, njl.
Isampulu: igqabi lesityalo
Ubungakanani: Ububanzi 2-3mm (indlela ngqo), ububanzi 5-7mm (indlela lysis)
Uluhlu lokuchongwa: iqhekeza ekujoliswe kulo ≤1kb