Tyala igqabi Direct PCR kit-UNG (ngaphandle kweZixhobo zeSampulu) iProtokholi eNgqo ngqo kwiPCR evela kwiMathiriyeli yeSityalo
Iinkcazelo
Ngokusekelwe kwikhithi yePlant Leaf Direct PCR, i-dUTP isetyenziswa endaweni ye-dTTP, kwaye i-enzyme ye-UNG enokuthoba itemplate equkethe i-dUTP yongezwa.Ngale ndlela, ukuchaneka kunye nokuchaneka kokukhulisa kuyaqinisekiswa, kwaye ingxaki yokungcoliswa kwemveliso ye-PCR enokuthi yenzeke ngexesha lovavanyo olukhulu lofuzo luthintelwe.
Iinkcukacha
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Amacandelo ekhithi
Icandelo I | Isithinteli P1 |
Isithinteli P2 | |
6×DNA Ilayisha isithinteli | |
Icandelo II | 2× Igqabi PCR EasyTMUmxube(UNG) |
Iimpawu&izinto eziluncedo
■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi
■ imathiriyeli encinci
■ I-Simple-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya
■ 2 × Ukuxuba, ukunciphisa iimpazamo zesampulu zokulayisha kunye nexesha lokulungiselela inkqubo yokusabela
■ Ulungiselelo olukhawulezayo lwetemplate lunokugqityezelwa kwimizuzu eli-10, ukusabela kwe-PCR kunokugqityezelwa ubuncinane kwimizuzu engama-50.
■ Ukusabela okuphezulu kwe-throughput-lysis kunokugqitywa kwi-96-well PCR plate
Iiparamitha zeKit
Isicelo: ukuchongwa okuguqulwe ngokwemfuza, i-genotyping, njl.
Isampulu: igqabi lesityalo
Ubungakanani: Ububanzi 2-3mm (indlela ngqo), ububanzi 5-7mm (indlela lysis)
Uluhlu lokuchongwa: iqhekeza ekujoliswe kulo ≤1kb
Ukuhamba komsebenzi
Electropherogram
Iiveki ezimbini ze-PCR Mix iziphumo zokufumanisa phantsi kwemeko yongcoliseko lwemveliso ye-PCR.