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Tyala igqabi Direct PCR kit-UNG (ngaphandle kweZixhobo zeSampulu) iProtokholi eNgqo ngqo kwiPCR evela kwiMathiriyeli yeSityalo

Inkcazelo yeKit:

Sebenzisa ngokuthe ngqo iqabunga okanye i-lysate yeqabunga njengetemplate yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.

Akukho xesha lidla kwaye libiza kakhulu ukucocwa kweDNA (Ixesha, umsebenzi kunye neendleko zigcinwe!)

Izinto ezincinci

Elula-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya

I-2 X Mix-ukunciphisa iimpazamo zokulayisha isampuli kunye nexesha lokulungiselela inkqubo yokusabela

Ukulungiswa ngokukhawuleza kwe-template kunokugqitywa kwimizuzu eyi-10, ukuphendula kwe-PCR kunokugqitywa kwimizuzu engama-50

I-high throughput-lysis reaction ingagqitywa kwi-96-well PCR plate

amandla angaphambili


Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcazelo

Ngokusekelwe kwikhithi yePlant Leaf Direct PCR, i-dUTP isetyenziswa endaweni ye-dTTP, kwaye i-enzyme ye-UNG enokuthoba itemplate equkethe i-dUTP yongezwa.Ngale ndlela, ukuchaneka kunye nokuchaneka kokukhulisa kuyaqinisekiswa, kwaye ingxaki yokungcoliswa kwemveliso ye-PCR enokuthi yenzeke ngexesha lovavanyo olukhulu lofuzo luthintelwe.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I

Isithinteli P1

Isithinteli P2

6×DNA Ilayisha isithinteli

Icandelo II

2× Igqabi PCR EasyTMUmxube(UNG)

Iimpawu&izinto eziluncedo

■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi
■ imathiriyeli encinci
■ I-Simple-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya
■ 2 × Ukuxuba, ukunciphisa iimpazamo zesampulu zokulayisha kunye nexesha lokulungiselela inkqubo yokusabela
■ Ulungiselelo olukhawulezayo lwetemplate lunokugqityezelwa kwimizuzu eli-10, ukusabela kwe-PCR kunokugqityezelwa ubuncinane kwimizuzu engama-50.
■ Ukusabela okuphezulu kwe-throughput-lysis kunokugqitywa kwi-96-well PCR plate

Iiparamitha zeKit

Isicelo: ukuchongwa okuguqulwe ngokwemfuza, i-genotyping, njl.
Isampulu: igqabi lesityalo
Ubungakanani: Ububanzi 2-3mm (indlela ngqo), ububanzi 5-7mm (indlela lysis)
Uluhlu lokuchongwa: iqhekeza ekujoliswe kulo ≤1kb

Ukuhamba komsebenzi

Tyala igqabi Ngqo PCR Kit-UNG4

Electropherogram

Tyala Igqabi Ngqo PCR Kit-UNG5

Iiveki ezimbini ze-PCR Mix iziphumo zokufumanisa phantsi kwemeko yongcoliseko lwemveliso ye-PCR.


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