I-China Plant igqabi I-PCR Plus ikhithi ethe ngqo (ngaphandle kweZixhobo zokuSampula) iProtokholi eNgqo ngqo kwi-PCR evela kuMvelisi weZityalo kunye noMboneleli |I-Foregene

Tyala igqabi Direct PCR Plus ikhithi (ngaphandle kweZixhobo zeSampulu) iProtokholi ethe ngqo kwiPCR evela kwiMathiriyeli yeSityalo

Tyala igqabi Direct PCR Plus ikhithi (ngaphandle kweZixhobo zeSampulu) iProtokholi ethe ngqo kwiPCR evela kuMfanekiso weSityalo oFanelekileyo

Inkcazelo yeKit:

Sebenzisa ngokuthe ngqo i-polysaccharide kunye ne-polyphenol leaf lysate njengethemplate ye-PCR yokukhulisa, ngaphandle kokutsalwa okuhlukeneyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.

◮Akukho xesha lidla kwaye libiza kakhulu ukucocwa kweDNA (Ixesha, umsebenzi kunye neendleko zigcinwe!)

◮Izinto ezincinci

◮Elula-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya

◮I-2 X Mix-ukunciphisa iimpazamo zokulayisha isampuli kunye nexesha lokulungiselela inkqubo yokusabela

◮Ukulungiswa ngokukhawuleza kwe-template kunokugqitywa kwimizuzu eyi-10, ukuphendula kwe-PCR kunokugqitywa kwimizuzu engama-50

◮I-high throughput-lysis reaction ingagqitywa kwi-96-well PCR plate

Thumela i-imeyile kuthi Khuphela njengePDF

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcazelo

Le mveliso isebenzisa inkqubo ye-lysis buffer eyodwa yokukhupha amagqabi esityalo.I-lysate ingasetyenziselwa njenge template ngaphandle kokuhlanjululwa.Emva kokongeza i-primers, i-PCR Mix yale khithi ingasetyenziselwa ukukhulisa.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I	Isithinteli PS1
	Isithinteli P2
	6×DNA Ilayisha isithinteli
Icandelo II	Buffer PS2
Icandelo II	2× Igqabi PCR Easy^TMXuba

Iimpawu&izinto eziluncedo

■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi
■ imathiriyeli encinci
■ I-Simple-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya
■ 2 × Ukuxuba, ukunciphisa iimpazamo zesampulu zokulayisha kunye nexesha lokulungiselela inkqubo yokusabela
■ Ulungiselelo olukhawulezayo lwetemplate lunokugqityezelwa kwimizuzu eli-10, ukusabela kwe-PCR kunokugqityezelwa ubuncinane kwimizuzu engama-50.
■ Ukusabela okuphezulu kwe-throughput-lysis kunokugqitywa kwi-96-well PCR plate