Imbewu yaseTshayina yokuTyalwa (i-Polysaccharide Polyphenol etyebileyo, encinci) I-PCR eNgqongileyo kunye neKit I-UNG(ngaphandle kweZixhobo zeSampulu) uMvelisi kunye noMboneleli |I-Foregene

Imbewu yeTyalo(i-Polysaccharide Polyphenol etyebileyo, encinci) I-PCR ethe ngqo kunye neKit I-UNG(ngaphandle kweZixhobo zokuSampula)

Imbewu yeTyalo(iPolysaccharide Polyphenol etyebileyo, encinci) I-PCR ethe ngqo yeKhithi ye-I-UNG(ngaphandle kweZixhobo zokuSampula) Umfanekiso okhoyo

Inkcazelo yeKit:

Sebenzisa ngokuthe ngqo i-polysaccharide polyphenol seed lysate njenge template yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.

- Akukho ukuchitha ixesha kunye neendleko zokucocwa kweDNA.

- Imfuno yesampulu incinci, thatha nje imbewu enye.

- Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.

-Isistim ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezela okunamandla kwi-PCR reaction inhibitors.

- Inkqubo ye-PCR yokuchasana nokungcoliswa kwe-2 × Imbewu ye-PCR Easy TM Mix (UNG), enokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcazelo

Le mveliso isebenzisa inkqubo yokusabela ye-lysis ekhethekileyo, enokuthi ikhuphe ngokukhawuleza i-DNA ye-genomic kwiisampuli zembewu yezityalo kunye ne-polysaccharides ephezulu kunye ne-polyphenols (ezifana neebhanana, umqhaphu, njl.) ukuze zisetyenziswe kwiimpendulo ze-PCR, ngoko ke ifaneleka ngakumbi kuvavanyo lwemfuzo olukhulu.Ukuze kuhlangatyezwane neemfuno zeemvavanyo ezahlukeneyo zokubona, le khithi inokusebenzisa iindidi ezininzi zeesampulu (ezifana nembewu epheleleyo, ukusikwa kwezicubu ezincinci, okanye iisampuli zomhlaba zembewu ezininzi) kwiimvavanyo.Phakathi kwazo, kwiisampuli zembewu ezisafuna ukuntshula, unokukhetha ukusika iziqwenga zezicubu ezincinci (1-5mg) ngaphandle kweembumba zembewu zovavanyo;kwimifuniselo efuna iisampulu kunye novavanyo kwinani elikhulu leesampuli, ungakhetha ukuxuba iisampulu ezininzi kwaye uzidibanise.Emva kokugaya kwiinqununu ezinobubanzi obumalunga ne-0.5mm, uvavanyo lunokwenziwa (ubuncinci i-0.1% yesampuli ekujoliswe kuyo inokufunyanwa).

Iinkcukacha

50T,200T,500T,2000T

Amacandelo ekhithi

Tyala iMbewu ngqo PCR Plus Kit I-UNG Imbewu yeSityalo sePolysaccharide Polyphenol (Encinci) Ngqo PCR Kit-UNG
Ukwakhiwa kweKit		TP-0316T	TP-03161	TP-03162	TP-03163
Ukwakhiwa kweKit		50 T	200 T	500 T	2000 T
Icandelo I	Isithinteli SSP1	4.5ml	18ml	45ml	90ml×2
	Isithinteli SP2	3ml	10ml	25ml	100ml
	I-6 × i-DNA yokulayisha i-Buffer	1.5ml	1.5ml	1.5ml	1.5ml×4
Icandelo II	Isithinteli SSP2	1.5ml	6ml	15ml	60ml
Icandelo II	2× Imbewu PCR EasyTM Mix	500μl	1ml×2	1.7ml×3	1.7ml×12
Imanyuwali		1	1	1	1

Iimpawu kunye neenzuzo

- Akukho ukuchitha ixesha kunye neendleko zokucocwa kweDNA.

- Imfuno yesampulu incinci, thatha nje imbewu enye.

- Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.

-Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors..

- Inkqubo yePCR yokuchasana nongcoliseko 2× Imbewu PCR Easy^TM Ukuxuba (UNG), enokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.