Ukulungiswa ngokukhawuleza kwezicubu zesampulu zezilwanyana ezisanda kuthathwa, zithintela umsebenzi we-RNase, zikhusele i-RNA ekuthotyweni.
-Ama-reagents asetyenziswa ngokuthe ngqo, inyathelo elinye likhona, kwaye i-RNA iyazinziswa ngokukhawuleza kwaye ikhuselwe.
-Ukusebenza kwiqondo lobushushu begumbi, kukulungele, kukhuselekile kwaye akukho tyhefu.
-Ukulahla inkathazo yomkhenkce owomileyo okanye ifriji ephantsi yobushushu bokugcina izicubu.
-I-tissue inokugcinwa ixesha elide ngaphandle komngcipheko wokuthotywa kwe-RNA, iqinisekisa idatha yeprofayili ye-gene ethembekileyo.