I-RT Easy II (kunye ne-gDNase) i-Master Premix yokuqala-strand ye-cDNA synthesis yeXesha lokwenyani PCR ene-gDNase
Inkcazelo yeKit:
-Ikhono elisebenzayo lokususa i-gDNA, enokususa i-gDNA kwi-template ngaphakathi kwemizuzu emi-2.
-Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu eyi-15 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-Iitemplates eziyinkimbinkimbi: iitemplates ezinomxholo ophezulu weGC kunye nesakhiwo sesibini esiyinkimbinkimbi sinokuphinda sitshintshwe ngokusebenza okuphezulu.
-I-High-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
I-reverse transcription system inozinzo oluphezulu lwe-thermal, iqondo lokushisa elichanekileyo liyi-42℃, kwaye isenomsebenzi olungileyo wokukhutshelwa umva kwi-50℃.
Iinkcukacha
25×20µl rxns,100×20µl rxns
RT EasyTMII (Nge-gDNase) yinkqubo yokukhuphela ebuyela umva eyenzelwe i-PCR yeXesha lokwenyani ukususa ngokukhawuleza ukungcoliseka kwe-genomic DNA.I-5 × gDNase Mix inokususa ngokukhawuleza i-genome eseleyo kwi-RNA kwi-42 ° C kwimizuzu ye-2, igweme ngokufanelekileyo ukuphazamiseka kwe-genome kwiziphumo ze-qPCR.
5 × RO-EasyTMUmxube uqulathe iForegene Reverse Transcriptase ephuhliswe ngokukodwa nguForegene, luhlobo olutsha lwe-reverse transcriptase enobudlelwane obunamandla be-RNA.Inkqubo ephuculweyo yenza ireverse transcription rate ngokukhawuleza, kwaye i-strand yokuqala ye-cDNA synthesis ingagqitywa kwi-42 ° C kwimizuzu eyi-15.
Inkqubo yokukhuphela umva yale khithi inokugcina umsebenzi olungileyo kuma-50°C.Ke ngoko, kunenzuzo ngakumbi kwiitemplate ezintsonkothileyo ze-RNA kwaye zinokubhala ngokulula iitemplates ze-RNA ezinomxholo ophezulu weGC kunye nesakhiwo sesibini esintsonkothileyo.
Amacandelo ekhithi
| 5× Umxube we-gDNase |
| 5 × RO-EasyTMXuba |
| RNase-Free ddH2O |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Ikhono elisebenzayo lokususa i-gDNA, enokususa i-gDNA kwi-template ngaphakathi kwemizuzu emi-2.
-Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu eyi-15 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-Iitemplates eziyinkimbinkimbi: iitemplates ezinomxholo ophezulu weGC kunye nesakhiwo sesibini esiyinkimbinkimbi sinokuphinda sitshintshwe ngokusebenza okuphezulu.
-I-High-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
I-reverse transcription system inozinzo oluphezulu lwe-thermal, iqondo lokushisa elichanekileyo liyi-42℃, kwaye isenomsebenzi olungileyo wokukhutshelwa umva kwi-50℃.
Isicelo seKit
Isetyenziswe ngokuthe ngqo kwi-Real Time PCR ukuhlalutya ngokobungakanani ukubonakaliswa kofuzo.
Inokukhawuleza nangokuchanekileyo ukuhlalutya umkhondo we-RNA njengentsholongwane ye-RNA.
Reverse transcription ye RNA templates with high GC content or complex complex structure.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
Ikhithi kufuneka igcinwe kwi -20°C.Gcina imveliso kwifriji ehlala rhoqo kwi-20 ° C ngokukhawuleza emva kokufunyanwa.Ukuba iimeko zokugcina zifanelekile, imveliso ayiyi kuthoba nayiphi na intsebenzo ngexesha le-1 leminyaka yokuqinisekisa.
