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  • I-RT-qPCR Easyᵀᴹ (Inyathelo elinye)-SYBR Green I

    I-RT-qPCR Easyᵀᴹ (Inyathelo elinye)-SYBR Green I

    ◮Ikhithi enenyathelo elinye yenza ukukhutshelwa umva kunye ne-qPCR iimpendulo ezimbini kwityhubhu enye, idinga kuphela ukongeza i-template ye-RNA, iiprimers ezithile ze-PCR kunye ne-RNase-Free ddH.2O.

    ◮Ikhithi inokukhawuleza kwaye ngokufanelekileyo ihlalutye i-RNA yentsholongwane okanye ilandele i-RNA.

    ◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.

    ◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.

    ◮RT-qPCR EasyTM(Inyathelo elinye) -SYBR Green I kit iza ne-ROX yedayi yesalathiso yangaphakathi, engasetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesignali phakathi kwamaqula, ekulungele ukuba abathengi basebenzise kwiimodeli ezahlukeneyo zezixhobo ze-PCR zobungakanani.

    amandla angaphambili

    ukubuzaiinkcukacha
  • RT-qPCR Easyᵀᴹ (Inyathelo elinye)-Taqman

    RT-qPCR Easyᵀᴹ (Inyathelo elinye)-Taqman

    - Nye-Ikiti yenyathelo yenza ukuba uguqulelo oluguqulelwe kunye ne-PCR luqhutywe kwityhubhu enye, kufuneka kuphela ukongeza i-template ye-RNA, iiprimers ezithile ze-PCR kunye ne-RNase-Free ddH.2O.

    - Uhlalutyo lwexesha langempela lobungakanani bentsholongwane yeRNA okanye umkhondo weRNA unokuqhutywa ngokukhawuleza nangokuchanekileyo.

    - Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.

    -Inkqubo yokusabela eyongeziweyo yenza ukusabela kube novakalelo oluphezulu lokubona, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.

    - RT-qPCR EasyTM(Inyathelo elinye) -Ikhithi yeTaqman iza ne-ROX yedayi yesalathiso yangaphakathi, enokusetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesiginali phakathi kwamaqula, elungele ukusetyenziswa kwabasebenzisi bokugqibela kwiimodeli ezahlukeneyo zezixhobo zePCR zobungakanani.

    amandla angaphambili

    ukubuzaiinkcukacha
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