I-SARS-CoV-2 Nucleic Acid yeKit (iMultiplex PCR Fluorescent Probe Method)
Inkcazelo yeKit:
◮ I-PCR ethe ngqo:Akukho mfuneko yekhithi yokutsalwa kwe-nucleic acid kunye neNkqubo, igqityiwe i-96 isampuli kwiyure eyi-1 okanye njalo.
◮ Imfuneko yesixhobo esiphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu:Kufuneka kuphela inkqubo yePCR yexesha lokwenyani .
◮ I-LoD kunye novakalelo oluphezulu:Ukubona ubuntununtunu kunokufikelela kwelona nqanaba lisezantsi njengeekopi ezingama-500/ml.
Ukusetyenziswa okucetywayo
Umgaqo wovavanyo
Le primer kunye neeseti zophando ziyilelwe ukuchongwa kolandelelwano olugciniweyo lwe-SARS-CoV-2 (ORF1ab gene, N gene and E gene).Ikhithi iqulethe uLawulo lwangaphakathi olusetyenziselwa ukuhlola umgangatho womzekelo.Itekhnoloji ye-PCR yexesha langempela isebenzisa i-polymerase chain reaction (PCR) ukukhulisa ulandelelwano oluthile olujoliswe kuyo kunye ne-probes ekujoliswe kuyo ekubhaqweni kwe-RNA eyandisiweyo.Iiprobe zibhalwe ngentatheli ye-fluorescent kunye nedayi yokucima.
Iinkcukacha eziKhawulezayo
◮Iinkcukacha:48 Rxns/kit, 96 Rxns/kit
◮ Ukucaciswa:96.72%
Uvakalelo:97.92%
Amacandelo
| Hayi. | Icandelo | Isixa | Isithako esiphambili | |||
| 48Rxns | 96 Rxns | |||||
| 1 | Ukukhutshwa kwe-Nucleic acidiarhente | 1.5 mL / ityhubhu | ityhubhu e-1 | 1.5 mL / ityhubhu | ityhubhu e-1 | I-surfactant |
| 2 | Umkhuseli we-RNA | 27 μL/ityhubhu | ityhubhu e-1 | 53 μL/ityhubhu | ityhubhu e-1 | I-RNase inhibitor |
| 3 | I-SARS-CoV-2isisombululo sokusabela | 800 μL/ityhubhu | ityhubhu e-1 | 1600 μL/ityhubhu | ityhubhu e-1 | Iprimer, probe, reaction buffer, dNTP |
| 4 | I-SARS-CoV-2umxube we-enzyme | 80 μL/ityhubhu | ityhubhu e-1 | 160 μL/ityhubhu | ityhubhu e-1 | Isiqalo esishushu seTaq enzyme,I-enzyme ye-M-MLV |
| 5 | I-SARS-CoV-2ulawulo olulungileyo | 100 μL/ityhubhu | ityhubhu e-1 | 100 μL/ityhubhu | ityhubhu e-1 | Recombinant plasmid equlathe targetiqhekeza, i-RNA |
| 6 | I-SARS-CoV-2ulawulo olubi | 1200 μL/ityhubhu | ityhubhu e-1 | 1200 μL/ityhubhu | ityhubhu e-1 | TE buffer |
Iimpawu&izinto eziluncedo
Kusetyenziswa kwanjani
Indlela yoku-1: Indlela ye-PCR ethe ngqo
Indlela yesi-2: I-Viral RNA Isolation + PCR
Indlela yoku-1: Indlela ye-PCR ethe ngqo usebenzisaIarhente yokukhupha iSampuli
Inkqubo yokufumanisa
Cold Chain Transport and Storage
Itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;
Beka ubomi kwishelufa
1 unyaka
Ukuodola Ulwazi
