I-SARS-CoV-2 iKit yeNucleic Acid eyahlukileyo (I) (iMultiplex PCR Fluorescent Probe Method)-yokubhaqwa kwezinto eziguqukayo ezisuka eBrazil, eMzantsi Afrika, nase-UK.
Inkcazelo yeKit:
◮ I-PCR ethe ngqo:Akukho mfuneko yekhithi yokutsalwa kwe-nucleic acid kunye neNkqubo, igqityiwe i-96 isampuli kwiyure eyi-1 okanye njalo.
◮ Imfuneko yesixhobo esiphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu:Kufuneka kuphela inkqubo yePCR yexesha lokwenyani .
◮ I-LoD kunye novakalelo oluphezulu:Ukubona ubuntununtunu kunokufikelela kwelona nqanaba lisezantsi njengeekopi ezingama-500/ml.
Iminyaka eyi-20+ yamava okuVelisa i-R&D 25+ eyona mpembelelo iphezulu
I-1000+ ii-reagents zeelebhu ezahlukeneyo 100+ iqabane ledyunivesithi
400+ abasasazi 2000+ SCI ucaphulo of reagents lab
Iinkcukacha
48 Rxns/kit, 96 Rxns/kit
| Hayi. | Icandelo | Isixa | Isithako esiphambili | |||
| 48Rxns | 96 Rxns | |||||
| 1 | I-agent yokukhululwa kwe-Nucleic acid | 5.3 mL/ ibhotile | 1 ibhotile | 5.3 mL/ibhotile | 2 ibhotile | I-surfactant |
| 2 | Umkhuseli we-RNA | 53 μL/ityhubhu | ityhubhu e-1 | 106 μL/ityhubhu | ityhubhu e-1 | I-RNase inhibitor |
| 3 | I-SARS-CoV-2 isisombululo sokusabela A | 800 μL/ityhubhu | ityhubhu e-1 | 1600 μL/ityhubhu | ityhubhu e-1 | Iprimer, probe, reaction buffer, dNTP |
| 4 | I-SARS-CoV-2 isisombululo sempendulo B | 800 μL/ityhubhu | ityhubhu e-1 | 1600 μL/ityhubhu | ityhubhu e-1 | Iprimer, probe, reaction buffer, dNTP |
| 5 | Umxube we-enzyme ye-SARS-CoV-2 | 160 μL/ityhubhu | ityhubhu e-1 | 320 μL/ityhubhu | ityhubhu e-1 | Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV |
| 6 | I-SARS-CoV-2 ulawulo olulungileyo | 100 μL/ityhubhu | ityhubhu e-1 | 100 μL/ityhubhu | ityhubhu e-1 | I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA |
| 7 | Ulawulo olubi lwe-SARS-CoV-2 | 1200 μL/ityhubhu | ityhubhu e-1 | 1200 μL/ityhubhu | ityhubhu e-1 | TE buffer |
Qaphela:Izixhobo ezisuka kwiikiti ezahlukeneyo azinakusetyenziselwa ukutshintshiselana.
Iimpawu&izinto eziluncedo:
Ngokukhawuleza kwaye kulula: gqibezela uvavanyo malunga neyure enye
Gwema ilahleko ye-nucleic acid: akukho mfuneko yokucocwa kwe-nucleic acid
Umda wokubona okuphantsi kunye novakalelo oluphezulu, ngakumbi i-suitabe yokubhaqwa okukhawulezileyo okukhulu
Imfuneko yezixhobo eziphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu
Ukuqonda ukuqonda kunokufikelela kwinqanaba eliphantsi njengeekopi ze-500 / mL).
Kusetyenziswa kwanjani:
1.Isixhobo esisebenzayo
ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S
2.Inkqubo yokufumanisa
Isampulu yokusetyenzwa: Isampuli ye-Naopharyngeal okanye i-oropharyngeal swab (inyathelo eli-1 kuphela)Linda imizuzu eyi-10)
PCR amplificaton(<=55min)
Umsebenzi:
Ugcino:
Itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;
Gcina kwi-2-8 ℃ emva kokuvulwa, sebenzisa phakathi kweentsuku ezi-7;kunqande ukunyibilika okuphindaphindiweyo (<5 amaxesha)
Beka ubomi kwishelufa:1 unyaka
