I-SARS-CoV-2 Eyahlukileyo ye-Nucleic Acid yokubona iKit III (iMultiplex PCR Fluorescent Probe Method)-yokubhaqwa kweDelta mutants
Iinkcukacha
48 Rxns/kit, 96 Rxns/kit
Hayi. | Icandelo | Isixa | Isithako esiphambili | |||
48Rxns | 96 Rxns | |||||
1 | I-agent yokukhululwa kwe-Nucleic acid | 1.4 mL / ityhubhu | 2 iityhubhu | 5.3 mL/ibhotile | 1 ibhotile | I-surfactant |
2 | Umkhuseli we-RNA | 53 μL/ityhubhu | ityhubhu e-1 | 106 μL/ityhubhu | ityhubhu e-1 | I-RNase inhibitor |
3 | Ukwahluka kwe-SARS-CoV-2 isisombululo sokusabela A | 800 μL/ityhubhu | ityhubhu e-1 | 1600 μL/ityhubhu | ityhubhu e-1 | Iprimer, probe, reaction buffer, dNTP |
4 | Umxube we-enzyme ye-SARS-CoV-2 | 160 μL/ityhubhu | ityhubhu e-1 | 320 μL/ityhubhu | ityhubhu e-1 | Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV |
5 | I-SARS-CoV-2 ulawulo olulungileyo | 100 μL/ityhubhu | ityhubhu e-1 | 100 μL/ityhubhu | ityhubhu e-1 | I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA |
6 | Ulawulo olubi lwe-SARS-CoV-2 | 1200 μL/ityhubhu | ityhubhu e-1 | 1200 μL/ityhubhu | ityhubhu e-1 | TE buffer |
Iimpawu&izinto eziluncedo:
Ngokukhawuleza kwaye kulula: gqibezela uvavanyo malunga neyure enye
Gwema ilahleko ye-nucleic acid: akukho mfuneko yokucocwa kwe-nucleic acid
Umda wokubona okuphantsi kunye novakalelo oluphezulu, ngakumbi i-suitabe yokubhaqwa okukhawulezileyo okukhulu
Imfuneko yezixhobo eziphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu
Ukuqonda ukuqonda kunokufikelela kwinqanaba eliphantsi njengeekopi ze-500 / mL).
Kusetyenziswa kwanjani:
1.Isixhobo esisebenzayo
ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S
2.Inkqubo yokufumanisa
Isampulu yokusetyenzwa: Isampuli ye-Naopharyngeal okanye i-oropharyngeal swab (inyathelo eli-1 kuphela)Linda imizuzu eyi-10)
PCR amplificaton(<=55min)
Umsebenzi:
Ugcino:
Itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;
Gcina kwi-2-8 ℃ emva kokuvulwa, sebenzisa phakathi kweentsuku ezi-7;kunqande ukunyibilika okuphindaphindiweyo (<5 amaxesha)
Beka ubomi kwishelufa:1 unyaka