IViral DNA & RNA Isolation Kit Viral DNA kunye neRNA Extraction yokuLungiselela iiKhithi zokuLungisa
Iinkcukacha
50 Preps, 200 Preps
I-Viral RNA I-Nucleic Acid Ukucoca I-Isolation Kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA yentsholongwane kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid, kunye ne-cell culture supernatant.Ikiti yongeza ngokukodwa i-Linear Acrylamide, enokuthi ibambe ngokulula ixabiso elincinci le-RNA kwiisampuli.IKholam ye-RNA Kuphela inokubopha i-RNA ngokufanelekileyo.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.
Ikhithi yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer viRW1 kunye ne-Buffer viRW2 inokuqinisekisa ukuba i-viral nucleic acid efunyenweyo ayinaprotheyini, i-nuclease okanye enye into engcolileyo, enokusetyenziswa ngokuthe ngqo kwimifuniselo yebhayoloji yemolekyuli esezantsi.
Amacandelo ekhithi
| Umgca Acrylamide |
| Isithinteli DRL |
| Isithinteli i-RW1, i-Buffer RW2 |
| RNase-Free ddH2O |
| Ikholamu yeDNA/RNA |
| Imiyalelo |
Iimpawu&izinto eziluncedo
■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Isivuno esiphezulu se-nucleic acid: I-DNA / RNA-iKholamu kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-DNA kunye ne-RNA.
■ Umthamo omkhulu wokusetyenzwa kwesampulu: ukuya kuthi ga kwi-200μl iisampulu zisenokwenziwa ngexesha ngalinye.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-20.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.
Isicelo seKit
Ifanelekile ukukhutshwa kunye nokucocwa kwe-viral nucleic acid kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid kunye ne-cell culture supernatant.
Ukuhamba komsebenzi
Umzobo
Ugcino kunye nobomi beShelf
■ Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
■ Isisombululo seLinear Acrylamide sinokugcinwa kwiqondo lobushushu legumbi kangangeentsuku ezisi-7;emva kokufumana ikiti, nceda uyikhuphe kwaye uyigcine kwi-20°C.
■ Emva kokongeza i-Linear Acrylamide kwi-Buffer DRL, inokugcinwa kwi-2-8 ° C ukuya kwi-48h.Nceda usebenzise isisombululo esele senziwe.
Isikhokelo sokuHlalutya ingxaki
Oku kulandelayo kuhlalutyo lweengxaki ezinokujamelana nazo ekutsalweni kwe-viral DNA / RNA, ngethemba lokuba luncedo kwiimvavanyo zakho.Ukongeza, kwezinye iingxaki zovavanyo okanye zobugcisa ngaphandle kwemiyalelo yokusebenza kunye nohlalutyo lwengxaki, sinikezele ngenkxaso yobugcisa ukukunceda.Ukuba unazo naziphi na iimfuno, nceda uqhagamshelane nathi: 028-83360257 okanye i-imeyile:
Tech@foregene.com.
Akukho kukhutshwa kwe-nucleic acid okanye isivuno esiphantsi se-nucleic acid
Ngokuqhelekileyo kukho izinto ezininzi ezichaphazela ukusebenza kakuhle kokubuyisela, njenge: isampuli ye-nucleic acid umxholo, indlela yokusebenza, umthamo we-lution, njl.
Uhlalutyo lwezizathu eziqhelekileyo:
1. Ukuhlamba iqhwa okanye ukushisa okuphantsi (4 ° C) centrifugation kwenziwa ngexesha lenkqubo.
Ingcebiso: Sebenza kwiqondo lobushushu begumbi (15-25°C) kuyo yonke le nkqubo, musa ukuhlamba umkhenkce kunye ne-centrifugation yobushushu obuphantsi.
2. Isampuli igcinwe ngokungafanelekanga okanye isampuli igcinwe ixesha elide.
Isincomo: Gcina iisampuli kwi-80 ° C kwaye ugweme ukukhenkceza ngokuphindaphindiweyo kunye nokunyibilika;zama ukusebenzisa iisampulu ezisanda kuqokelelwa ukutsalwa kwe-nucleic acid.
3. I-lysis yesampuli eyaneleyo.
Isincomo: Nceda uqinisekise ukuba isampula kunye nesisombululo sokusebenza se-lysis sixutywe ngokucokisekileyo kwaye sifakwe kwindawo yokushisa (15-25 ° C) imizuzu eyi-10.
4. Ukongezwa okungalunganga kwe-eluent.
Ingcebiso: Qinisekisa ukuba i-ddH2O ye-RNase-Free yongezwa phakathi kwe-membrane yekholomu yokucoca, kwaye ungayilahli kwiringi yekholomu yokucoca.
5. Umthamo ochanekileyo we-ethanol ngokupheleleyo awuzange wongezwe kwi-Buffer RW2.
Ingcebiso: Nceda ulandele imiyalelo, yongeza umthamo ochanekileyo we-ethanol epheleleyo kwi-Buffer RW2 kwaye udibanise kakuhle phambi kokusebenzisa ikhithi.
6. Umthamo wesampuli engafanelekanga.
Iingcebiso: 200µl yesampulu isetyenzwa kwi-500µl nganye ye-Buffer DRL.Ukusetyenzwa kweesampulu ezigqithisileyo kuya kubangela isivuno esisezantsi se-nucleic acid extraction.
7. Umthamo wesandi esingafanelekanga okanye i-elution engaphelelanga.
Isincomo: Umthamo ocacileyo wekholamu yokucoca yi-30-50μl;ukuba i-elution effect ayinelisi, kuyacetyiswa ukuba kwandiswe ixesha kwiqondo lobushushu legumbi emva kokongeza i-ddH2O ye-RNase-Free preheated, efana ne-5-10min.
8. I-Ethanol ihlala kwikholamu emva kokuhlamba nge-Buffer RW2.
Isiphakamiso: Ukuba i-ethanol ihlala emva kwe-centrifugation kunye ne-Buffer RW2 imizuzu ye-2, ikholamu inokubekwa kwindawo yokushisa kwemizuzu emi-5 emva kwe-centrifugation ukususa ngokupheleleyo i-ethanol eseleyo.
I-nucleic acid ecocekileyo iyancipha
Umgangatho we-nucleic acid ecocekileyo inxulumene nokugcinwa kwesampuli, ukungcoliswa kwe-RNase, ukusebenza kunye nezinye izinto.Uhlalutyo lwezizathu eziqhelekileyo:
1. Iisampuli eziqokelelweyo azigcinwanga ngexesha.
Isiphakamiso: Ukuba isampuli ayisetyenziswanga ngexesha emva kokuqokelela, nceda uyigcine kwi -80 ° C kwiqondo lokushisa eliphantsi ngokukhawuleza.Ukukhutshwa kwe-RNA, zama ukusebenzisa iisampulu ezisanda kuqokelelwa.
2. Qokelela iisampuli kwaye umkhenkce kwaye unyibilike ngokuphindaphindiweyo.
Isiphakamiso: Gwema ukukhenkceza kunye nokunyibilika (kungekho ngaphezu kweyodwa) ngexesha lokuqokelela kunye nokugcinwa kweesampuli, ngaphandle koko isivuno se-nucleic acid siya kuncitshiswa.
3. I-RNase yaziswa kwigumbi lokusebenza okanye iiglavu ezilahlwayo, iimaski, njl.njl. azinxitywa.
Ingcebiso: Imifuniselo yokutsalwa kwe-RNA yenziwa ngcono kwigumbi lokusebenza elahlukileyo le-RNA, kwaye itafile yelabhoratri kufuneka icocwe phambi kovavanyo.
Nxiba iiglavu ezilahlwayo kunye nemaski ngexesha lovavanyo ukunqanda ukuthotywa kwe-RNA okubangelwa kukwaziswa kwe-RNase kowona mgangatho mkhulu.
4. I-reagent ingcolisekile nge-RNase ngexesha lokusetyenziswa.
Ingcebiso: Faka endaweni entsha yeViral DNA/RNA Isolation Kit ukulungiselela imifuniselo enxulumeneyo.
5. Iityhubhu ze-centrifuge kunye neengcebiso ze-pipette ezisetyenziselwa ukuguqulwa kwe-RNA zingcolile nge-RNase.
Ingcebiso: Qinisekisa ukuba iityhubhu ze-centrifuge, iingcebiso ze-pipette, iipayipi, njl njl. ezisetyenziselwa ukukhutshwa kwe-RNA zonke zi-RNase-Free.
I-asidi ye-nucleic ecocekileyo ichaphazela iimvavanyo ezisezantsi
I-DNA kunye ne-RNA ehlanjululwe yikholomu yokucoca, ukuba i-ion yetyuwa kunye neprotheyini iphezulu kakhulu, iya kuchaphazela iimvavanyo ezisezantsi, ezifana: ukukhulisa i-PCR, ukuguqulelwa kwe-reverse transcription, njl.
1. I-DNA ekhutshiweyo kunye ne-RNA zineeyoni zetyuwa ezishiyekileyo.
Isiphakamiso: Qinisekisa ukuba umthamo ochanekileyo we-ethanol epheleleyo yongezwa kwi-Buffer RW2, kwaye uhlambe ikholamu yokucoca kabini kwisantya se-centrifugation esichazwe kwimiyalelo yokusebenza;Yenza i-centrifugation ukunciphisa ukungcoliseka kwe-ion yetyuwa.
2. I-DNA ekhutshiwe kunye ne-RNA zineentsalela ze-ethanol.
Isiphakamiso: Emva kokuqinisekisa ukuhlamba nge-Buffer RW2, yenza i-tube centrifugation engenanto kwisantya se-centrifugation kwimiyalelo yokusebenza;ukuba kusekho intsalela ye-ethanol, unako ukwenza i-centrifuge ityhubhu engenanto kwaye uyibeke kwindawo yokushisa kwemizuzu emi-5 ukususa intsalela ye-ethanol ukuya kwinqanaba elikhulu.
Izikhokelo zoMyalelo:
I-Viral DNA & RNA Isolation Kit Manual Manual
