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I-Zebra yeNtlanzi ngqo kwi-PCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwi-zebrafish kunye nezinye izicubu zentlanzi zamanzi amatsha, amaphiko omsila okanye iisampulu zamaqanda entlanzi kwiimpendulo ze-PCR, ke kufanelekile ngokukodwa kuvavanyo lwemfuza olukhulu. Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65 ° C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwePCR.
Umxube we-2 × we-PCR Easy TM unonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye unokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokufanelekileyo nangokukhulisa okuthile. Le reagent iqulethe ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. Isetyenziswe ngokudibeneyo ne-lysis buffer inokufumana ngokukhawuleza iisampulu ngokukhawuleza, kwaye ineempawu zobuntununtunu obuphezulu, ukucaciswa okunamandla, kunye nozinzo olulungileyo.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
