Ukusetyenziswa ngokuthe ngqo kwe-zebrafish lysate njenge template yokukhulisa i-PCR, akukho kukhutshwa kwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Kongezwe inkqubo ye-UNG yokuchasana nokungcoliseka kongcoliseko ukuze kupheliswe iimpawu zobuxoki.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.