Zimbini iindidi eziphambili zemingcipheko kwiilabhoratri ze-PCR: iingozi ze-biosafety kunye nobungozi bongcoliseko lwe-nucleic acid.Eyangaphambili ilimaza abantu kunye nokusingqongileyo, kwaye okokugqibela kuchaphazela iziphumo zovavanyo lwe-PCR.Eli nqaku limalunga nePCR labhoratri yokubeka iliso kumngcipheko kunye namanqanaba omngcipheko ahambelanayo aziswe kuwe.

01 ICandelo lelabhoratri yePCR

1. Ilebhu yovavanyo lwebhayoloji yemolekyuli

Ngokweemfuno zeSiqendu 1.1 seMigangatho yokuSeta iSiseko seLabhoratri yoVavanyo lweGene yoKliniki, iilebhu zePCR ngokuqhelekileyo ziquka iindawo ezine: indawo yokugcina i-reagent kunye nendawo yokulungiselela, indawo yokulungiselela i-specimen, indawo yokukhulisa, kunye nommandla wohlalutyo lwemveliso.Ukuba indlela ye-PCR yexesha langempela ye-fluorescent isetyenzisiweyo, indawo yokukhulisa kunye nommandla wokuhlalutya inokudibaniswa kwindawo enye;ukuba i-analyzer ye-PCR ezenzekelayo isetyenzisiweyo, indawo yokulungiselela i-specimen, indawo yokukhulisa kunye nendawo yokuhlalutya inokudibaniswa kwindawo enye.

“Incwadi yoMsebenzi yoVavanyo lweNucleic Acid eNtsha yeCoronavirus kuMaziko oNyango (uTrial Version 2)” imisela ukuba ngokomgaqo, iilabhoratri ezenza uvavanyo lwe-nucleic acid entsha ye-coronavirus kufuneka zimisele ezi ndawo zilandelayo: indawo yokugcina i-reagent kunye nendawo yokulungiselela, indawo yokulungiselela isampula, indawo yokukhulisa kunye nohlalutyo lwemveliso.Ezi ndawo zintathu kufuneka zizimele ngokupheleleyo omnye komnye kwindawo ebonakalayo, kwaye akunakubakho unxibelelwano oluthe ngqo nomoya.

2. Igumbi lokulungiselela isampuli

Nangona iisampulu zinokulungiswa ngokulula kwindawo yokulungiselela umfuziselo, igumbi elikhethekileyo lokulungiselela isampulu lisafuneka xa kujongwa iisampuli ezintsonkothileyo kunye nenani elikhulu leisampulu.Igumbi lokulungiselela isampula linomngcipheko omkhulu wokhuseleko lwezinto eziphilayo kunye nokungcoliswa kwe-nucleic acid.

3. Igumbi lokucoca inkunkuma

Ukunyangwa kwenkunkuma ngokungafanelekanga kuya kuzisa imingcipheko emikhulu yokhuseleko lwendalo kunye nongcoliseko lwe-nucleic acid elabhoratri.Ngoko ke, igumbi lokucoca inkunkuma kufuneka libekwe esweni rhoqo.

02 Iindawo zokubeka iliso kumngcipheko kwiilebhu zePCR

Iilebhu ezahlukeneyo zohlulwe ngokwesampulu yegumbi lokulungiselela, indawo yokugcina i-reagent kunye nendawo yokulungiselela, indawo yokulungiselela umzekelo, indawo yokukhulisa kunye nohlalutyo lwemveliso, kunye negumbi lokunyanga inkunkuma.

Ngokohlobo lwendawo yesampulu, yahlulwe kwindawo, isixhobo, isampuli, ukubeka iliso kokusingqongileyo kunye nepipette.

Inqanaba lomngcipheko liqala phantsi ukuya phezulu ukusuka kwenkwenkwezi enye★ ukuya kwiinkwenkwezi ezintathu★★★.

1. Igumbi lokulungiselela isampula:

Isetyenziselwa ukubhaliswa, ukulungiswa kunye nokungasebenzi kweesampuli, kwaye umngcipheko wokhuseleko lwebhayoloji ngowona uphezulu.Ngenxa yokuba iisampuli azicatshulwanga kwaye zinyuswe, ngaphandle kweepipettes ezihlala zidibana neesampuli, umngcipheko wokungcoliswa kwe-nucleic acid kwezinye iindawo ziphantsi.

1-4 Iisampulu kwiindawo zokubeka iliso

5-8 Iisampulu kwindawo yokubeka iliso

9-12 indawo yokubeka iliso isampuli

1. Indawo yogcino kunye nendawo yokulungiselela:

Isetyenziselwa ukulungiswa kwee-reagents zokugcina, ukuhanjiswa kwee-reagents kunye nokulungiswa komxube wokusabela kwe-amplification, kunye nokugcinwa kunye nokulungiswa kwezinto ezisetyenziswayo ezifana neetyhubhu ze-centrifuge kunye neengcebiso zepipette.Akukho qhagamshelwano oluthe ngqo kunye neesampuli kwaye akukho nucleic acid efanelekileyo kule ndawo, ngoko ingozi ye-biosafety kunye ne-nucleic acid yokungcoliseka ingozi iphantsi.

13-16 Iisampulu kwiindawo zokubeka iliso

17-22 Iisampulu ekubekeni iliso
3. Yenza umzekelo weendawo zokulungiselela

Isetyenziselwa ukuvula i-barrel yokudlulisa, ingasebenzi i-specimen (xa isebenzayo), ikhuphe i-nucleic acid kwaye uyifake kwi-tube yokusabela kwe-amplification, njl.

29 Iisampulu kwiindawo zokubeka iliso

4. Ukwandisa kunye nohlahlelo lwemveliso:

Isetyenziselwa ukukhulisa i-nucleic acid.Lo mmandla awubandakanyi ukusetyenzwa kwesampulu, kwaye umngcipheko wokhuseleko lwebhayoloji uphantsi.I-nucleic acid amplification ikakhulu kule ndawo, kwaye umngcipheko wokungcoliswa kwe-nucleic acid uphezulu kakhulu.

38 Iisampulu kwiindawo zokubeka iliso
5. Igumbi lokucoca inkunkuma:

Isetyenziselwa ukusetyenzwa koxinzelelo oluphezulu lweesampulu.Imingcipheko yokhuseleko lwebhayoloji ebandakanyeka ekusetyenzweni kweesampulu kule ndawo iphezulu kakhulu.Iimveliso zokukhulisa i-nucleic acid ziyacetyiswa ukuba ziphathwe njengenkunkuma yonyango.Uxinzelelo oluphezulu alukhuthazwa, kwaye umngcipheko wokungcoliswa kwe-nucleic acid uphantsi.

43-44 Iisampulu kwiindawo zokubeka iliso

03 Phumeza

Ngeli xesha sidwelise iindawo zokubeka iliso ezingama-44.Kuqikelelwa ukuba abantu abaninzi kufuneka babuze, ngaba kufuneka benze amanqaku amaninzi?Ewe, yenza konke!Ndicebisa ukuba uqale wenze uvavanyo lomngcipheko kwilabhoratri yakho, olunokuthi lwenziwe ngokomngcipheko ukusuka phezulu ukuya ezantsi, unokubeka iliso kuhlobo olufanayo lweesampulu kunye, okanye unokuphuhlisa isicwangciso sesampulu sokubeka iliso rhoqo.Ngamafutshane, ilabhoratri nganye inokwenza isicwangciso sayo sokuphumeza ngokusekelwe kwimeko yayo.Owona mngcipheko mkhulu wokuvavanya iilabhoratri kukungahoyi umngcipheko.