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I-RT-PCR Easyᵀᴹ I(Inyathelo elinye)
◮Ikiti yenyathelo elinye yenza ukuba uguqulelo loguqulelo kunye nePCR luqhutywe kwityhubhu enye.Ifuna kuphela ukongeza ithemplate ye-RNA, iiprimers ezithile zePCR kunye ne-RNase-Free ddH2O.
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
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