ISilwanyana sisonke se-RNA Isolation Kit Iyonke yeRNA yokutsalwa kunye neeKits zokuCoca kwiiTissues zezilwanyana kunye neSeli
Inkcazelo yeKit:
Ngokukhawuleza nangempumelelo ukukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu ezahlukeneyo zezilwanyana.
Akukho mfuneko yakukhathazeka malunga nokonakaliswa kwe-RNA.Yonke inkqubo i-RNase-Free
Susa ngokufanelekileyo i-DNA usebenzisa i-DNA-Cleaning Column
Susa i-DNA ngaphandle kokongeza i-DNase
Elula-yonke imisebenzi igqitywe kwiqondo lokushisa
Ukukhawuleza-ukusebenza kunokugqitywa kwimizuzu engama-30
Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
Ukucoceka okuphezulu-OD260 / 280≈1.8-2.1
Iinkcazelo Kits
50 Preps, 200 Preps
Le kit isebenzisa ispin ikholamu kunye nefomulaiphuhliswe yinkampani yethu, enokukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle okuphezulu.Inika iKholamu yokucoca i-DNA esebenzayo, enokwahlula ngokulula kwaye ibhengeze i-genomic DNA kwi-supernatant kunye ne-tissue lysate, elula. kunye nokonga ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.
Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.
Amacandelo ekhithi
| Animal Total RNA Isolation Kit | ||
| Amacandelo ekhithi | RE-03011 | RE-03014 |
| 50 T | 200 T | |
| Isithinteli RL1* | 25ml | 100ml |
| Isithinteli RL2 | 15ml | 60ml |
| Isithinteli RW1* | 25ml | 100ml |
| Isithinteli RW2 | 24ml | 96ml |
| RNase-Free ddH2O | 10ml | 40ml |
| IKholam ye-RNA kuphela | 50 | 200 |
| Ikholamu yokucoca iDNA | 50 | 200 |
| Incwadi Yemiyalelo | Iqhekeza eli-1 | Iqhekeza eli-1 |
Ulwazi lwemveliso
| Ifomathi | Spin ikholamu | Icandelo lokucoca | Ikholamu ye-Foregene, i-reagent |
| Flux | 1-24 iisampulu | Ixesha lokulungiselela | ~30 min (iisampulu ezingama-24) |
| Centrifuge | Desk centrifuge | Ukuhlukana kwepyrolysis | Ukwahlukana kwe-Centrifugal |
| Isampulu | Izicubu zezilwanyana;iseli | Isixa seesampuli | Izicubu: 10-20 mg;Iseli:(1-5)×106 |
| Umthamo weElution | 50-200 μL | Ubuninzi bomthamo wokulayisha | 850 μL |
Iimpawu&izinto eziluncedo
■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1
Isicelo seKit
Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.
Iiparamitha zemveliso
■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×106
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl
