Izilwanyana zizonke i-RNA Isolate Kit
Iinkcazo zeKhithi
Le khithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yinkampani yethu, enokukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA epheleleyo kwizicubu ezahlukeneyo zezilwanyana ngokusebenza kakuhle.Ibonelela ngekholamu efanelekileyo yokuCoca iDNA, enokwahlula ngokulula kunye ne-adsorb ye-genomic DNA ukusuka kwi-supernatant kunye ne-lysate yethishu, elula kunye nokonga ixesha; Ikholamu ye-RNA-kuphela enokubopha ngokufanelekileyo i-RNA kwaye inokuqhubekeka ngaxeshanye kunye nefomula eyahlukileyo yeesampulu ezininzi.
Yonke le nkqubo ayi-RNase-Simahla, ukuze i-RNA ekhutshiweyo ingathotywa; I-Buffer RW1, iBuff RW2 inkqubo yokuhlamba i-buffer, ukuze i-RNA efumanekayo ingabinayo iprotein, i-DNA, ion kunye nongcoliseko lwekhompawundi.
Iimpawu kunye nezibonelelo
■ Akukho sidingo sokukhathazeka ngokuhla komgangatho weRNA; yonke le nkqubo ayi-RNase-Simahla
■ Susa ngokuchanekileyo iDNA-usebenzisa iDNA-Ukucoca ikholamu
■ Susa i-DNA ngaphandle kokongeza i-DNase
■ Yonke imisebenzi elula igqityiwe kubushushu begumbi
■ Ukusebenza ngokukhawuleza kunokugqitywa ngemizuzu engama-30
■ ekhuselekileyo-akukho reagent ephilayo efunekayo
■ Ukucoceka okuphezulu -OD260 / 280≈1.8-2.1
Isicelo seKiti
Kukulungele ukukhutshwa nokucocwa kwe-RNA iyonke kwiintlobo ezahlukeneyo zezicubu zezilwanyana okanye ezifriziweyo okanye iiseli ezikhulisiweyo.
Iiparamitha zemveliso
■ Ukusetyenziswa okungaphantsi: umsinga wokuqala we-cDNA synthesis, RT-PCR, cloning cloning, Northern Blot, njl.
■ Iisampulu: izicwili zezilwanyana, iiseli ezikhulisiweyo
■ Isikali: Izicubu ze-10-20mg, iiseli (2-5) × 106
■ Ubuninzi be-DNA yokubopha amandla ekholamu yokucoca: 80 μg
■ Ubungakanani be-Elution: 50-200 μl
Ukuhamba komsebenzi
Umzobo
Izilwanyana zizonke i-RNA Isolate Kit iphathwe nge-20mg
Iisampulu ezintsha zeempuku, thabatha i-5% ye-RNA eyi-1% ye-agar
IGlycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo
Ukugcinwa kunye neshelf life
Ikhithi ingagcinwa iinyanga ezingama-24 kubushushu begumbi (15-25 ℃) okanye 2-8 ℃ ixesha elide. I-Buffer RL1 inokugcinwa kwi-4 ℃ kangangenyanga enye emva kokudibanisa β- mercaptoethanol (ngokuzithandela).