Izilwanyana zizonke i-RNA Isolate Kit

Izilwanyana zizonke i-RNA Isolate Kit

Inkcazo yeKiti:

Izixhobo zeKit:

IBuffer RL1, iBuff RL2 

IBuffer RW1, iBuffer RW2

RNase-Simahla ddH2O, Ikholamu yokucoca i-DNA

Ikholamu ye-RNA-kuphela

Ukucaciswa:

50 Preps, 200 Preps


Iinkcukacha Product

Iimpawu zemveliso

Iinkcazo zeKhithi

Le khithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yinkampani yethu, enokukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA epheleleyo kwizicubu ezahlukeneyo zezilwanyana ngokusebenza kakuhle.Ibonelela ngekholamu efanelekileyo yokuCoca iDNA, enokwahlula ngokulula kunye ne-adsorb ye-genomic DNA ukusuka kwi-supernatant kunye ne-lysate yethishu, elula kunye nokonga ixesha; Ikholamu ye-RNA-kuphela enokubopha ngokufanelekileyo i-RNA kwaye inokuqhubekeka ngaxeshanye kunye nefomula eyahlukileyo yeesampulu ezininzi.

Yonke le nkqubo ayi-RNase-Simahla, ukuze i-RNA ekhutshiweyo ingathotywa; I-Buffer RW1, iBuff RW2 inkqubo yokuhlamba i-buffer, ukuze i-RNA efumanekayo ingabinayo iprotein, i-DNA, ion kunye nongcoliseko lwekhompawundi.

Iimpawu kunye nezibonelelo

■ Akukho sidingo sokukhathazeka ngokuhla komgangatho weRNA; yonke le nkqubo ayi-RNase-Simahla
■ Susa ngokuchanekileyo iDNA-usebenzisa iDNA-Ukucoca ikholamu
■ Susa i-DNA ngaphandle kokongeza i-DNase
■ Yonke imisebenzi elula igqityiwe kubushushu begumbi
■ Ukusebenza ngokukhawuleza kunokugqitywa ngemizuzu engama-30
■ ekhuselekileyo-akukho reagent ephilayo efunekayo
■ Ukucoceka okuphezulu -OD260 / 280≈1.8-2.1

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Isicelo seKiti

Kukulungele ukukhutshwa nokucocwa kwe-RNA iyonke kwiintlobo ezahlukeneyo zezicubu zezilwanyana okanye ezifriziweyo okanye iiseli ezikhulisiweyo.

Iiparamitha zemveliso

■ Ukusetyenziswa okungaphantsi: umsinga wokuqala we-cDNA synthesis, RT-PCR, cloning cloning, Northern Blot, njl.
■ Iisampulu: izicwili zezilwanyana, iiseli ezikhulisiweyo
■ Isikali: Izicubu ze-10-20mg, iiseli (2-5) × 106
■ Ubuninzi be-DNA yokubopha amandla ekholamu yokucoca: 80 μg
■ Ubungakanani be-Elution: 50-200 μl

Ukuhamba komsebenzi

animal total RNA-simple workflow

Umzobo

3 Animal Total RNA Isolation Kit7

Izilwanyana zizonke i-RNA Isolate Kit iphathwe nge-20mg
Iisampulu ezintsha zeempuku, thabatha i-5% ye-RNA eyi-1% ye-agar

IGlycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye neshelf life

Ikhithi ingagcinwa iinyanga ezingama-24 kubushushu begumbi (15-25 ℃) okanye 2-8 ℃ ixesha elide. I-Buffer RL1 inokugcinwa kwi-4 ℃ kangangenyanga enye emva kokudibanisa β- mercaptoethanol (ngokuzithandela).


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