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ISilwanyana sisonke se-RNA Isolation Kit Iyonke yeRNA yokutsalwa kunye neeKits zokuCoca kwiiTissues zezilwanyana kunye neSeli

Inkcazelo yeKit:

Ngokukhawuleza nangempumelelo ukukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu ezahlukeneyo zezilwanyana.

Akukho mfuneko yakukhathazeka malunga nokonakaliswa kwe-RNA.Yonke inkqubo i-RNase-Free

Susa ngokufanelekileyo i-DNA usebenzisa i-DNA-Cleaning Column

Susa i-DNA ngaphandle kokongeza i-DNase

Elula-yonke imisebenzi igqitywe kwiqondo lokushisa

Ukukhawuleza-ukusebenza kunokugqitywa kwimizuzu engama-30

Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo

Ukucoceka okuphezulu-OD260 / 280≈1.8-2.1

amandla angaphambili


Iinkcukacha zeMveliso

Iithegi zeMveliso

FAQ

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ispin ikholamu kunye nefomulaiphuhliswe yinkampani yethu, enokukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle okuphezulu.Inika iKholamu yokucoca i-DNA esebenzayo, enokwahlula ngokulula kwaye ibhengeze i-genomic DNA kwi-supernatant kunye ne-tissue lysate, elula. kunye nokonga ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi

Animal Total RNA Isolation Kit
Amacandelo ekhithi RE-03011 RE-03014
50 T 200 T
Isithinteli RL1* 25ml 100ml
Isithinteli RL2 15ml 60ml
Isithinteli RW1* 25ml 100ml
Isithinteli RW2 24ml 96ml
RNase-Free ddH2O 10ml 40ml
IKholam ye-RNA kuphela 50 200
Ikholamu yokucoca iDNA 50 200
Incwadi Yemiyalelo Iqhekeza eli-1 Iqhekeza eli-1

 

Ulwazi lwemveliso

Ifomathi Spin ikholamu Icandelo lokucoca Ikholamu ye-Foregene, i-reagent
Flux 1-24 iisampulu Ixesha lokulungiselela ~30 min (iisampulu ezingama-24)
Centrifuge Desk centrifuge Ukuhlukana kwepyrolysis Ukwahlukana kwe-Centrifugal
Isampulu Izicubu zezilwanyana;iseli Isixa seesampuli Izicubu: 10-20 mg;Iseli:(1-5)×106
Umthamo weElution 50-200 μL Ubuninzi bomthamo wokulayisha 850 μL

 

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

iingenelo ze-foregene RNA Isolation kit

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×106
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

isilwanyana esipheleleyo se-RNA-ukuhamba komsebenzi okulula

Umzobo