ISilwanyana sisonke se-RNA Isolation Kit Iyonke yeRNA yokutsalwa kunye neeKits zokuCoca kwiiTissues zezilwanyana kunye neSeli
Iinkcazelo Kits
50 Preps, 200 Preps
Le kit isebenzisa ispin ikholamu kunye nefomulaiphuhliswe yinkampani yethu, enokukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle okuphezulu.Inika iKholamu yokucoca i-DNA esebenzayo, enokwahlula ngokulula kwaye ibhengeze i-genomic DNA kwi-supernatant kunye ne-tissue lysate, elula. kunye nokonga ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.
Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.
Amacandelo ekhithi
Animal Total RNA Isolation Kit | ||
Amacandelo ekhithi | RE-03011 | RE-03014 |
50 T | 200 T | |
Isithinteli RL1* | 25ml | 100ml |
Isithinteli RL2 | 15ml | 60ml |
Isithinteli RW1* | 25ml | 100ml |
Isithinteli RW2 | 24ml | 96ml |
RNase-Free ddH2O | 10ml | 40ml |
IKholam ye-RNA kuphela | 50 | 200 |
Ikholamu yokucoca iDNA | 50 | 200 |
Incwadi Yemiyalelo | Iqhekeza eli-1 | Iqhekeza eli-1 |
Ulwazi lwemveliso
Ifomathi | Spin ikholamu | Icandelo lokucoca | Ikholamu ye-Foregene, i-reagent |
Flux | 1-24 iisampulu | Ixesha lokulungiselela | ~30 min (iisampulu ezingama-24) |
Centrifuge | Desk centrifuge | Ukuhlukana kwepyrolysis | Ukwahlukana kwe-Centrifugal |
Isampulu | Izicubu zezilwanyana;iseli | Isixa seesampuli | Izicubu: 10-20 mg;Iseli:(1-5)×106 |
Umthamo weElution | 50-200 μL | Ubuninzi bomthamo wokulayisha | 850 μL |
Iimpawu&izinto eziluncedo
■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit
Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.
Iiparamitha zemveliso
■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×106
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl